肉鸡鸡胚肝脏中脂滴的发育研究

doi:10.16431/j.cnki.1671-7236.2017.03.011

Abstract

Abstract:

This study was aimed to explore the developmental change of lipid droplet in liver of chicken embryos.120 fertilized eggs were incubated, and 6 embryos were selected randomly every day and their livers were removed for measuring the formation and development of lipid droplets from E7 to E21 during the incubation.The methods of frozen section and Sudan Ⅲ staining were used. The results showed that:①Lipid droplets emerged early on the 9th day of chicken embryos incubation (E9). The quantities and sizes of lipid droplets became more and larger with the increasing of chicken embryos age. ②Quantities of lipid droplets in every hepatic lobule decreased from central veins to the margin of hepatic lobule along hepatic cords before E18, while more lipid droplets distributed at the margin of the liver than that in the center of liver after E18. ③The quantities and sizes of hepatic lipid droplets were significant difference during the three stage of E9-E14, E15-E17 and E18-E21 (P<0.05). The average sizes of hepatic lipid droplets were 3.2, 5.7 and 10.8 μm, respectively, and their average numbers were 67,327 and 397 n/mm², respectively. In conclusion, the quantities and sizes of hepatic lipid droplets became more and larger with the increasing of chicken embryos age.

Key words: chicken embryos; lipid droplet; liver; development

CLC Number:

S831.1

YU Yan, MA Yi-yuan, WU Shi-xiu, TAO Li-kui, ZHANG Yan-hong, XU Zhi-yong, MA Jin-you. Study on Development of Broiler Liver Lipid Droplet during Embryonic Period[J]. , 2017, 44(3): 692-697.

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Study on Development of Broiler Liver Lipid Droplet during Embryonic Period

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