3株H9N2亚型禽流感病毒全基因组序列进化分析

doi:10.16431/j.cnki.1671-7236.2015.02.006

Abstract

Abstract: In order to clarify the genetic variation of three different endogenous avian influenza viruses subtype H9N2 A/Chicken/Jilin/22/13 (JL22),A/Chicken/Jilin/24/13 (JL24) and A/Duck/Jilin/37/13 (JL37),eight gene fragments of the three viruses were amplified by RT-PCR,and then cloned and sequenced.The results showed that the main pathogenic genes of the three H9N2 subtype AIV belonged to the classic Eurasian species.Compared with A/Chicken/Hong Kong/G9/97 and A/Duck/Hong Kong/Y280/97,the amino acid sequence of HA of JL22 had a more potential glycosylation site at the location of 551.The amino acid residue at position 226 of HA of JL22,JL24 and JL37 were all Leu,sialic acid receptors in mammals had the same characteristics and it showed that it enhanced human infection.Asn substituted Ser phenomenon was occurred on M gene at 31 site,indicating that these viruses were resistant to amantadine.Phylogenetic trees could be seen three viruses distantly related strains,each gene belonged to a branch not a unity,and some genes from chicken,duck and swine origin influenza virus strains derived three kinds of highly homologous,it was speculated that three different strains of different animals were naturally rearrangement products after a long period of evolution .

Key words: H9N2 subtype avian influenza virus; genome; phylogenetic analysis

CLC Number:

S852.65

LIU Xin-xin, MENG Qing-feng, WANG Xue, YAO Gui-zhe, CONG Yan-long, WANG Wei-li. Phylogenetic Analysis of the Complete Genome Sequence of Three Strains H9N2 Subtype Avian Influenza Viruses[J]. , 2015, 42(2): 275-284.

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Phylogenetic Analysis of the Complete Genome Sequence of Three Strains H9N2 Subtype Avian Influenza Viruses

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