绵羊肺炎支原体人工感染SPF鸡胚的研究

doi:10.16431/j.cnki.1671-7236.2020.09.034

Abstract

Abstract: In order to evaluate the SPF chicken embryos as a candidate of the experimental model of Mycoplasma ovipneumoniae (Mo) infection,the 7-day-old SPF chicken embryos were infected with different concentrations of Mo(10⁸,10⁹ and 10¹⁰ ccu/mL) by injection of vitelline fluid and allantoic cavity.The mortality and Mo detection rate (PCR detection and isolation) of infected chicken embryos were employed to evaluate the optimal inoculation route,dose and sampling site.Three different isolates of Mo were submitted to injection of chicken embryos for pathogenicity comparison.The results showed that the optimal inoculation route and dose were vitelline fluid injection with 0.2 mL (10⁹ ccu/mL) Mo per embryo and the sampling site for detection was also vitelline fluid.Three Mo isolates revealed good infectivity and lethality to the chicken embryo.The chicken embryos mortality caused by FL3 strain (45%) and MoGH3-3 (40%) were both higher than that by A3 strain (25%),but with no significance (P>0.05).The detection rate of Mo from the chicken embryos in FL3 group (100%) was higher than that of MoGH3-3 strain(85%) and that of A3 strain (90%),but with no significance(P>0.05).This experiment preliminarily proved that the SPF chicken embryos could be infected and partially killed by Mo,and the virulence of different strains of Mo to the chicken embryos was different.The results provided the data for further establishment of the chicken embryo infection model of Mo,which would benefit the research on Mo pathogenicity and vaccine development.

Key words: Mycoplasma ovipneumoniae; infection; SPF chicken embryos

CLC Number:

S858.26

YUAN Ting, HAO Huafang, MA Lina, CHEN Shengli, YAN Xinmin, WU Yaqin, CAI Yating, SUN Yanming, CHU Yuefeng. Experimental Infection of Mycoplasma ovipneumoniae in SPF Chicken Embryos[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(9): 2988-2996.

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Experimental Infection of Mycoplasma ovipneumoniae in SPF Chicken Embryos

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