利用体外产气法评定不同比例氨化秸秆替代苜蓿的组合效应

doi:10.16431/j.cnki.1671-7236.2017.12.015

Abstract

Abstract:

This experiment was conducted to determine the best roughage combination of alfalfa meal (AM) and ammoniated corn straw (ACS),so as to improve the efficiency of roughage utilization and reduce the cost of feeding. The AM was mixed with ACS in the proportion of 100:0,80:20,60:40,50:50,40:60,20:80 and 0:100 with 3 replicates per group. The accumulated gas production (GP) at fermentation for 3,6,12,24,48 h,and the changes of pH,dry matter disappearance rate (DMD),ammonia nitrogen (NH₃-N) concentration, microbial protein (MCP) and volatile fat acids (VFA) concentration,and their single factor associative effects index (SFAEI) and multiply factors associative effects index (MFAEI) were determined by gas production technique in vitro after fermentation for 48 h. The results showed as follows:The accumulated gas production of AM20:ACS80 group was higher than that of the other groups,and the DMD of AM0:ACS100 group was significantly lower than other groups (P<0.05).The NH₃-N concentration of AM20:ACS80 and AM0:ACS100 groups was significantly higher than the other groups (P<0.05).The MCP concentration was highest in AM20:ACS80 group,and the concentration of acetic acid and TVFA in AM20:ACS80 group was the highest (P<0.05),and the acetic acid/propionic acid ratio in all groups was more than 3,which indicating that it belonged to the acetic acid fermentation type.The pH during the fermentation ranged from 6.69 to 6.85.The results of MFAEI and SFAEI of each combination indicated that only the AM20:ACS80 group showed the positive associative effects. In conclusion,the 20:80 combination ratio for AM and ACS showed the best associative effects.

Key words: alfalfa meal (AM); ammoniated corn straw (ACS); associative effects; rumen fermentation parameters

CLC Number:

S816.5

YU Man-man, JIANG Yu-xuan, ZHANG Mei-mei, XIE Xiao-lai, WANG Chen-xi. Evaluation of Associative Effects of Alfalfa Meal and Ammoniated Corn Straw by Gas Production Technique in vitro[J]. , 2017, 44(12): 3497-3504.

References

[1] Blümmel M,Schröder A,Südekum K H,et al. Estimating ruminal microbial efficiencies in silage-fed cattle:Comparison of an in vitro, method with a combination of in situ,and in vivo, measurements[J]. Journal of Animal Physiology & Animal Nutrition,2010,81(2):57-67.
[2] 张子仪.中国饲料学[M].北京:中国农业出版社,2000.
[3] ÇEtìNkaya N,Erdem F. Effects of different Juncus acutus:Maize silage ratios on digestibility and rumen cellulolytic bacteria[J].Kafkas Vniversitesi Veteriner Fakültesi Dergisi,2015,21(4):499-505.
[4] Niderkorn V,Martin C,Morvan A L,et al. Associative effects between fresh perennial ryegrass and white clover on dynamics of intake and digestion in sheep[J].Grass & Forage Science,2016,5:1-9.
[5] 高静,贾玉山,王晓光,等. 饲草组合效应综合指数研究[J].草业学报,2012,21(4):236-243.
[6] 李申发.苜蓿、黄贮及玉米秸秆组合效应研究[D].长春:吉林农业大学,2007.
[7] 李惠堂,李剑虹,陈冬华,等.快速加压氨化对稻秸超微组织结构的影响[J].中国畜牧杂志,2011,47(11):65-69.
[8] 林琳,金龙.快速氨化玉米秸秆对育肥羔羊生长的影响[J]. 养殖技术顾问,2008,5:140-141.
[9] 熊本海,罗清尧,赵峰,等.中国饲料成分及营养价值表(2015年第26版)制订说明[J].中国饲料,2015,21:23.
[10] Menke K,Steingass H.Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid[J].Animal Research and Development,1988,28(1):47-55.
[11] Schofield P,Pell A N. Measurement and kinetic analysis of the neutral detergent-soluble carbohydrate fraction of legumes and grasses[J]. Journal of Animal Science, 1995,73(11):3455-3563.
[12] 冯宗慈,高民.通过比色测定瘤胃液氨氮含量方法的改进[J].畜牧与饲料科学,2010,6:40-41.
[13] 胡伟莲.皂甙对瘤胃发酵与甲烷产量及动物生产性能影响的研究[D].杭州:浙江大学,2005.
[14] 王加启.反刍动物营养学研究方法[M].北京:现代教育出版社,2011.
[15] Menke K H,Raab L,Salewski A,et al. The estimation of the digestibility and metabolizable energy content of ruminant feeding stuffs from the gas production when they are incubated with rumen liquor in vitro[J].Journal of Agricultural Science,1979,93(1):217-222.
[16] 张锐,朱晓萍, 李建云,等.辽宁绒山羊常用粗饲料苜蓿和羊草间饲料组合效应[J].动物营养学报,2013,25(10):2481-2488.
[17] Pathak A K. Various factors affecting microbial protein synthesis in the rumen[J].Veterinary World, 2008,1(6):186-189.
[18] Shakarami F,Chaji M,Eslami M,et al. The comparison of in vitro digestibility of wheat straw by rumen anaerobic fungi of Khuzestan buffalo and Holstein cattle[J].Iranian Journal of Applied Animal Science,2015,5(2):285-292.
[19] 冯仰廉.反刍动物营养学[M].北京:科学出版社,2004.
[20] 王芳,徐元君,牛俊丽,等.体外产气法评价反刍动物饲料营养价值的研究[J].中国畜牧兽医,2016,43(1):76-83.
[21] 刘海霞,刘大森,隋美霞,等.羊常用粗饲料干物质和粗蛋白的瘤胃降解特性研究[J].中国畜牧杂志,2010,46(21):37-42.
[22] 李袁飞,郝建祥,马艳艳,等.体外瘤胃发酵法评定不同类型饲料的营养价值[J]. 动物营养学报,2013,25(10):2403-2413.
[23] 冯建芳,李秋凤,高艳霞,等.全株玉米青贮饲料、苜蓿及谷草间组合效应的研究[J]. 中国饲料,2017,2:10-15.
[24] Calsamiglia S,Ferret A,Devant M. Effects of pH and pH fluctuations on microbial fermentation and nutrient flow from a dual-flow continuous culture system[J].Journal of Dairy Science,2002,85(3):574-579.
[25] 王嘉厚.氨化稻草替代羊草对羔羊育肥效果及瘤胃发酵的影响[D].哈尔滨:东北农业大学,2009.
[26] Gunun P,Wanapat M,Anantasook N.Effects of physical form and urea treatment of rice straw on rumen fermentation,microbial protein synthesis and nutrient digestibility in dairy steers[J].Asian-Australasian Journal of Animal Sciences,2013,26(12):1689.
[27] Zhou X Q, Zhang Y D,Zhao M,et al.Effect of dietary energy source and level on nutrient digestibility,rumen microbial protein synthesis,and milk performance in lactating dairy cows[J].Journal of Dairy Science,2015,98(10):7209.
[28] 杨志林,李秋凤,曹玉凤,等.体外产气法评定全株玉米青贮与谷草的组合效应研究[J]. 中国畜牧兽医,2017,44(3):698-707.
[29] 焦光月,赵丽萍,白元生,等.体外产气法研究不同尿素水平对瘤胃发酵和甲烷产量的影响[J].中国畜牧杂志,2015,s1:66-70.
[30] 王震宇,韩士群,严少华,等.蓝藻厌氧发酵过程中若干指标的变化[J].江苏农业学报,2008,24(5):701-705.
[31] 隋美霞,刘海霞,王宗伟,等.体外法研究总挥发性脂肪酸和产气量与饲料营养成分的关系[J].中国饲料,2009,13:28-30.
[32] 张吉鹍,邹庆华,王金芬,等.稻草与多水平苜蓿混合瘤胃体外发酵组合效应的整体研究[J].饲料工业,2011,32(17):40-48.
[33] 袁翠林,于子洋,王文丹,等.豆秸、花生秧和青贮玉米秸间的组合效应研究[J].动物营养学报,2015,27(2):647-654.
[34] 韩肖敏,曹玉凤,李秋凤,等.用体外产气法评价玉米秸秆、稻草、玉米秸秆青贮与精料的组合效应[J].动物营养学报,2017,29(2):699-711.
[35] 周传社,汤少勋,姜海林,等.农作物秸秆体外发酵营养特性及其组合利用研究[J].应用生态学报,2005,16(10):1862-1867.

Evaluation of Associative Effects of Alfalfa Meal and Ammoniated Corn Straw by Gas Production Technique in vitro

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