China Animal Husbandry and Veterinary Medicine ›› 2020, Vol. 47 ›› Issue (8): 2677-2683.doi: 10.16431/j.cnki.1671-7236.2020.08.038

• Clinical Veterinary Medicine • Previous Articles     Next Articles

Comparison of Two Methods for Detecting Recessive Mastitis in Cows

LI Haiqiang1, YANG Dasheng2, LU Qi1, TANG Shaoxun2   

  1. 1. College of Animal Science, Guizhou University, Guiyang 550025, China;
    2. CAS Key Laboratory of Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China
  • Received:2020-01-17 Online:2020-08-20 Published:2020-08-15

Abstract: The aim of this paper was to detect recessive mastitis more accurately and to provide a reference for evaluating the change of recessive mastitis milk.The methods of California mastitis test (CMT) and bromine vanilla phenol blue test (BTB) were used in the present experiment to detect the recessive mastitis of 35 cows,and the pH,milk serum amyloid A (SAA),milk composition and blood cells composition were also detected and compared between two methods.The results showed that the positive rate detected by CMT method (40%) was higher than that of BTB method (34.3%),while the difference was not significant (P>0.05),and the match rate of CMT method (35.71%) was lower than that of BTB method (41.67%).The pH of milk in two positive groups were significantly higher (P<0.05) than that of negative group.The logarithms of somatic cell count of positive group detected by BTB test was significantly higher than that of negative group (P<0.05),which was numerically lower than that positive group detected by CMT test.The SAA content in the milk of positive group detected by BTB test was significantly lower than that of the negative group (P<0.05).There was no significant difference in the blood cell components among the two testing positive and negative groups (P>0.05).It was concluded that the CMT test was more sensitive than that of BTB test,while the BTB test was more accurate.

Key words: recessive mastitis; cows; CMT method; BTB method; milk composition

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