土鸡屠宰过程中大肠杆菌毒力基因检测及耐药性分析

doi:10.16431/j.cnki.1671-7236.2020.08.031

Abstract

Abstract: In order to explore the contamination,virulence genes carrying and antibiotic resistance of Escherichia coli(E.coli) in slaughtering process of free-range chicken.319 samples which came from 12 slaughter places of Wanzhou,Kaizhou,Wuxi,Fengjie district in Northeast Chongqing were collected in September 2018 to January 2019.These samples were analyzed by colony morphology,microscopic observation,biochemical test,PCR and drug sersitivity test.The results indicated that the isolation rate of E.coli was 22.57%(72/319).The isolation rates of restaurant,live poultry shop and slaughterhouse were 29.73%(22/74),25.00%(24/96) and 17.45%(26/149) respectively.The rates of samples of waste water,ground,feather,tools and carcass were 75.00%(18/24),21.62%(8/37),20.54%(23/112),17.65%(6/34) and 15.18%(17/112) respectively.All of the virulence genes were detected expect estA,estB and elt genes.The detection rate was 58.33%(42/72) and there were 14 combinations even 4 strains coexisted 4 virulence genes.The strains were most sensitive to amikacin and cefotaxime,and existed multi-antibiotic resistance seriously which most of them were from 7 to 10 folds.There was a high risk to be contaminated by pathogenic E.coli in slaughtering process of free-range chicken and existing multi-antibiotic resistance seriously.So the rational using of drugs and keeping a clean process of slaughter should pay more attention.

Key words: Escherichia coli(E.coli); slaughtering process; virulence genes; antibiotic resistance

CLC Number:

S859.1

CHEN Yaqiang, PENG Jinjin, LIAO Ming, LIAO Qinfeng. Virulence Genes Detection and Antibiotic Resistance Analysis of Escherichia coli Isolated from Slaughtering Process of Free-range Chicken[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2615-2624.

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Virulence Genes Detection and Antibiotic Resistance Analysis of Escherichia coli Isolated from Slaughtering Process of Free-range Chicken

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