江苏及周边地区鸭致病性大肠杆菌血清型、毒力因子及耐药研究

doi:10.16431/j.cnki.1671-7236.2020.12.032

Abstract

Abstract: In this study,191 strains of avian pathogenic Escherichia coli (APEC) were isolated from duck farms in and around Jiangsu province.The serotype,virulence gene distribution and drug resistance of 21 strains (one from each farm) were detected,and the correlation between serotype,virulence gene distribution and drug resistance was analyzed,in order to provide reference for the prevention and control of APEC.The serotypes of 21 APEC strains showed that there were 12 strains of O65,accounting for 57.14% of all strains.The results of virulence gene detection showed that 5 virulence genes had a high distribution rate,among which the positive rate of fimA gene was 100%,and the positive rates of ECs3737,ECs3703,tsh and irp2 genes were 90.5%,85.7%,57.1% and 42.9%,respectively.There were 6 strains (28.57%) with five virulence genes.The results of drug sensitivity test showed that 21 APEC strains had multiple drug resistance,and 100% strains were resistant to enrofloxacin,doxycycline,vancomycin and erythromycin.Among all the strains,85.71% and 14.29% were resistant to more than 10 and 21 kinds of drugs,respectively.The relationship among serotypes,virulence gene distribution and drug resistance showed that there were 13 strains with more than 4 virulence genes,9 of which were O65 serotypes.Among the 13 strains with more than 4 virulence genes,9 strains (69.23%) were resistant to more than 15 drugs,and 3 strains (23.08%) were resistant to more than 20 drugs.The results showed that the serotypes of Escherichia coli isolated from ducks in Jiangsu province and its surrounding areas were complex,carrying a variety of virulence genes,and the drug resistance was serious.

Key words: avian pathogenic Escherichia coli; serotype; virulence gene; antibiotic resistance

CLC Number:

S852.61

GUO Changming, WU Zhi, ZHU Shanyuan, WANG Yongjuan, FENG Qi, DONG Hongyan, YUAN Cheng, XU Hai, WU Shuang. Serotypes,Virulence Factors and Antibiotic Resistance of Duck Pathogenic Escherichia coli in Jiangsu Province and Its Surrounding Areas[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(12): 4076-4084.

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Serotypes,Virulence Factors and Antibiotic Resistance of Duck Pathogenic Escherichia coli in Jiangsu Province and Its Surrounding Areas

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