基于RNA-seq数据鉴定苏姜猪肉质性状相关基因

doi:10.16431/j.cnki.1671-7236.2019.10.001

Abstract

Abstract: In order to obtain the genes related to meat quality traits,the transcriptomes of longissimus dorsi muscle and leg muscle of three Sujiang pigs at 90- and 180-day-old were sequenced using RNA-seq technology.The differentially expressed genes of the longissimus dorsi muscle between 180- and 90-day-old Sujiang pigs,and the differentially expressed genes between the longissimus dorsi muscle and the leg muscle of 180-day-old Sujiang pigs were analyzed with the gene ontology (GO) and KEGG Pathway strategies.The results showed that there were 1 655 differentially expressed genes between two longissimus dorsi muscle tissues at different ages.GO functional and KEGG Pathway enrichment analysis revealed that 474 up-regulated genes were mainly involved in biological functions such as muscle fiber,transcriptional regulation,calcium signaling pathway,MAPK signaling pathway,insulin signaling pathway and FOXO signaling pathway,and 1 181 down-regulated genes were mainly involved in biological functions such as immunity,hematopoiesis,lysosomes,binding,and enzyme activities.There were 383 differentially expressed genes between the longissimus dorsi muscle and leg muscle of 180-day-old Sujiang pigs.GO functional and KEGG Pathway enrichment analysis revealed that no significant results were identified in the 70 expression up-regulated genes,and 313 down-regulated genes were mainly involved in cell differentiation and proliferation,muscle development,extracellular matrix,cGMP-PKG signaling pathway and other biological functions.In this experiment,the transcriptome information of the longissimus dorsi muscle and leg muscle tissues of Sujiang pigs were obtained,and 6 candidate genes (FOXO1,FOXO3,FOXO4,MYF6,A-FABP and H-FABP) related to meat quality traits were screened.This results laid a foundation for further study of the molecular mechanism of meat quality traits in Sujiang pigs.

Key words: RNA-seq; Sujiang pig; meat quality; longissimus dorsi muscle; leg muscle; candidate gene

CLC Number:

XU Pan, HE Xinxin, NI Ligang, TANG Zixue, YANG Chi, ZHU Shubin, JI Hongming, ZHOU Chunbao. Identification of Meat Quality Traits Related Genes in Sujiang Pig Based on RNA-seq Data[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(10): 2813-2822.

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Identification of Meat Quality Traits Related Genes in Sujiang Pig Based on RNA-seq Data

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