猪繁殖与呼吸综合征病毒与副猪嗜血杆菌混合感染的诊断

doi:10.16431/j.cnki.1671-7236.2019.06.035

Abstract

Abstract:

To ascertain the cause of the death of nursery pigs in Yangjiang city,Guangdong province,we carried out the bacteriological and drug sensitive test of 3 clinical samples (lung,liver and spleen) collected from pigs in this study.PCR/RT-PCR method were used to test pseudorabies virus (PRV),classical swine fever virus (CSFV),porcine reproductive and respiratory syndrome virus (PRRSV),circovirus type 2 (PCV2),circovirus type 3 (PCV3) and Mycoplasma hyopneumoniae in clinical samples.Futhermore,the nucleotide sequence of PRRSV ORF5 gene from three positive samples was determined,which conducted nucleotide sequence similarity analysis and constructed genetic evolution tree with VR2332,HuN4,JXA1,CH-1a and other representative strains.It indicated that a strain of Haemophilias paresis (Hps) was isolated and identified by bacteriological detection.Hps had strong sensitivity to 7 kinds of clinical drugs,such as amoxicilin and cefradine.The gene similarity analysis showed that the PRRSV ORF5 gene nucleotide similarity in three strains (LJW1,LJW2 and LJW3) was 99.3% to 99.8%,the nucleotide similarity to the European-type representative strain Lelystad was 64.0% to 64.2%,the nucleotide similarity with HP-PRRSV strains JXA1,HuN4,CH-1a and TJ were 99.2% to 99.5%,99.0% to 99.3%,94.5% to 94.9%,98.8% to 99.2%,respectively;The higher nucleotides similarity contrast with HP-PRRSV strains HeNzm1-16 and GXL205-2015 isolated from Henan and Guangxi in China were 99.3% to 99.7% and 99.2% to 99.7%,respectively.The nucleotide similarity with the American classic vaccine strains MLV,the American standard strains NC,and the American classic strain VR-2332 was 88.5% to 88.8%,85.2% to 85.5% and 82.3% to 82.6%,respectively.The phylogenetic tree analysis of PRRSV ORF5 showed that all the three strains belonged to the American strain,and they were in the same branch with the domestic HP-PRRSV representative strains JXA1,HuN4,TJ,etc,and the genetic relationship was the closer.This study revealed the pathogens of pigs in this field,and confirmed the genetic relationship between three isolates from PRRSV and different representative strains at the molecular level,which provided a basis of the rational selection and use of attenuated vaccines and comprehensive prevention and control PRRSV.

Key words: porcine reproductive and respiratory syndrome virus(PRRSV); Haemophilus parasuis(Hps); ORF5 gene; genetic evolution

CLC Number:

S855.3

ZHANG Hailong, CHEN Shengnan, XIE Bo, LI Li, HUANG Liangzong. Diagnosis of Mixed Infection of Porcine Reproductive and Respiratory Syndrome Virus and Haemophilus parasuis[J]. , 2019, 46(6): 1856-1864.

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Diagnosis of Mixed Infection of Porcine Reproductive and Respiratory Syndrome Virus and Haemophilus parasuis

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