云南楚雄规模化猪场仔猪大肠杆菌HPI基因调查及耐药性分析

doi:10.16431/j.cnki.1671-7236.2019.06.034

Abstract

Abstract:

In order to study the epidemiological characteristics and drug resistance of HPI gene of E.coli,feces and anal swabs were collected from Chuxiong local pig farms in Yunnan province.The isolation and identification of E.coli were carried out by McConkay medium,biochemical identification tube and PCR amplification.The HPI gene carrying status,pathogenicity and drug resistance of the isolates were studied by PCR reaction,animal regression test and disk diffusion method respectively.The results showed that 78 strains of E.coli were isolated,the isolation rate was 83.87%,and the carrying rate of HPI gene was 96.15%.Within 24 h,the mortality rate of HPI-positive strains was 100%,and that of HPI-negative strains was 75%.No mice died in the control group,suggesting that HPI gene could increase the mortality rate of mice.Drug susceptibility tests showed that the drug resistance rates to tetracycline,ampicillin,compound sinomenine,kanamycin,gentamicin,ceftazidime and amikacin were 88.46%,84.62%,73.08%,53.85%,38.46%,19.23% and 5.13%,respectively.Colistin B showed no drug resistance.The results showed that the epidemic situation of HPI gene in Chuxiong prefecture of Yunnan province was optimistic,and it could provide a theoretical basis for the prevention and treatment of E.coli-related diseases.

Key words: HPIgene; irp2 gene; pathogenicity; resistance

CLC Number:

S858.28

JING Linxi, GAO Feilong, SHAN Chunlan, LIU Chaoying, WANG Shiyu, YAN Yulin, GAO Hong. Investigation of HPI Gene and Drug Resistance Analysis of Piglets Escherichia coli in Large-scale Pig Farms in Chuxiong,Yunnan Province[J]. , 2019, 46(6): 1849-1855.

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Investigation of HPI Gene and Drug Resistance Analysis of Piglets Escherichia coli in Large-scale Pig Farms in Chuxiong,Yunnan Province

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