云南地区牛源无乳链球菌分离鉴定及毒力基因和耐药性的检测

doi:10.16431/j.cnki.1671-7236.2018.11.034

Abstract

Abstract:

This experiment was aimed to study the biological characteristics of Streptococcus agalactiae (S.agalactiae)and provide a theoretical basis for the prevention and treatment of cow mastitis caused by S.agalactiae.S.agalactiae was separated and identified based on bacterial molecular biology;according to GenBank-registered S.agalactiae 16S rRNA,genus-specific cfb (CAMP factor),virulence gene and drug resistance gene sequences,14 pairs of primers were designed using Oligo 6.0 and Primer Premier 5.0 softwares,then,a rapid PCR detection method was established;The resistance tests of 20 common antibiotic drugs was carried out.The results showed that 17 strains of S.agalactiae were successfully identified,and the virulence genes were highly homologous to the corresponding sequences of S.agalactiae reported on NCBI,which were ≥ 99%;A total of 6 drug resistance genes were detected;The resistance rates of the isolates to penicillin,erythromycin,lincomycin,clindamycin,vancomycin,ampicillin,novobiocin and sulfisoxazole were higher,and the drug resistance rates were 100%,94.1%,94.1%,94.1%,94.1%,82.3%,82.3% and 47.1%,respectively,severely resistant to penicillin;And the resistance rates to aminoglycosides,tetracyclines,cephalosporins and quinolones were low,which were 15.7%,14.7%,7.7% and 3.9%,respectively.This study results indicated that the established PCR rapid detection method was sensitive and reliable,and there was a multi-drug resistance of some β-lactams,macrolides,sulfonamides and other antibiotics in Yunnan.

Key words: dairy cow mastitis; Streptococcus agalactiae; virulence gene; drug resistance

CLC Number:

S852.61^＋1

ZHU Yu, LU Lu, YAN Xingqiong, GONG Lei, SUN Weixing, QU Weijie. Isolation, Identification and Detection of Virulence Genes and Drug Resistance of Streptococcus agalactiae from Cattle in Yunnan[J]. , 2018, 45(11): 3261-3270.

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Isolation, Identification and Detection of Virulence Genes and Drug Resistance of Streptococcus agalactiae from Cattle in Yunnan

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