龙须菜对异育银鲫消化吸收、免疫及抗氧化性能的影响

doi:10.16431/j.cnki.1671-7236.2018.06.013

Abstract

Abstract:

The objective of this study was to evaluate the effects of the diets which used the Gracilaria lemaneiformis to substitute the rapeseed meal on the digestive absorption, immunity and antioxidant performance of juvenile Carassius auratus gibelio,to determine the optimal replacement scope of the Gracilaria lemaneiformis.In this study, 450 tail of uniform juvenile Carassius auratus gibelio were randomly divided into 6 groups with 3 replicates per group and 25 fish per replicate.The fish of the control group were fed with the basic diet without Gracilaria lemaneiformis,the test diets which used the 3% (D1),6% (D2),9% (D3), 12% (D4) and 15%(D5) Gracilaria lemaneiformis to substitute the rapeseed meal were used to feed the experimental fish for 8 weeks,respectively.The protein and fat content of all the diets were equal,and at the end of the experiment,the activities of enzyme involved in digestive absorption, immunity and antioxidant function were determined.The results indicated that compared with the control group, there were no significant differences in the activities of digestive enzymes in the liver of the fish from each group (P<0.05).The activities of the alkaline phosphatase (ALP) and the superoxide dismutase (SOD) in the liver of the fish from D1 group, the activities of the γ-glutamyl-transferase (γ-GT) in the anterior intestine of the fish from D1 and D2 groups were significantly increased (P<0.05),while the activities of the lipase (LPS),ALP and SOD of the fish from D5 group were decreased significantly (P<0.05).The activities of the acid phosphatase (ACP) in the kidney of the fish from D1 to D3 groups, the activities of serum lysozyme of the fish from D2 to D5 groups and the serum total cholesterol (TC) content and the total antioxidant capacities (T-AOC) in the anterior intestine of the fish from D2 and D3 groups were significantly increased (P<0.05),while the serum total protein (TP) content of the fish from D5 group were decreased greatly (P<0.05).The results indicated that a small amount addition of the Gracilaria lemaneiformis (3% to 6%) would improve the digestion,absorption and immune function of the Carassius auratus gibelio to some extent, when the substitute amount of the Gracilaria lemaneiformis in the diet up to 15%,the negative impacts on the digestive and absorption capacities as well as the health status of the fish would appear.

Key words: Gracilaria lemaneiformis; Carassius auratus gibelio; digestion and absorption; immunity; antioxidant performance

CLC Number:

S963

XUAN Xiongzhi, LI Wenjia, LU Yubiao. Effects of Gracilaria lemaneiformis in Diets on Digestion and Absorption, Immunity and Antioxidant Performance of Juvenile Carassius auratus gibelio[J]. , 2018, 45(6): 1526-1534.

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Effects of Gracilaria lemaneiformis in Diets on Digestion and Absorption, Immunity and Antioxidant Performance of Juvenile Carassius auratus gibelio

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