不同中药对中性粒细胞迁移微血管内皮细胞后溶菌酶释放量的影响

doi:10.16431/j.cnki.1671-7236.2018.01.031

Abstract

Abstract:

To investigate the effect of lipopolysaccharide (LPS) stimulating rat intestinal mucous membrane microvascular cells (RIMVECs) on lysozyme content of transendothelial migration of neutrophil (PMNs) and the intervention of Pulsatillae (BTW),Coptis chinensis (HL),Cortex phellodendri (HB),Cortex fraxini (QP) and Purslane (MCX),0,0.1,1,10,100 μg/mL LPS were selected to stimulate the RIMVECs in this experiment,and the IL-6 level in cells culture supernatants at 12 and 24 h were determined by ELISA assay to determine the proper concentration of LPS for subsequent trials. To establish a transwell model of PMNs trans RIMVECs adherence and migration,the cell adhesion,migration rate and the release of lysozyme were evaluated by cell counts,staining and ELISA methods after LPS stimulating RIMVECs.The results showed that compared with the control group,the level of IL-6 at 24 h was extremely significantly increased (P<0.001) after RIMVECS was stimulated by 10 μg/mL LPS.Compared with LPS group,the PMNs adhesion rate in HL and BTW groups,the migration rate of QP and BTW groups were significantly decreased (P<0.05),and the amount of lysozyme in HL and BTW groups were extremely significantly increased (P<0.001).The results showed that LPS stimulating endothelial cells could significantly inhibite the release of lysozyme bactericidal enzymes,intervention of Pulsatillae and Coptis chinensis could significantly reduce this inhibition.This study suggested that the release of lysozyme might be related to the functional integrity of endothelial cells,providing a theoretical basis for further screening of water-soluble components in effective Chinese herbal medicine.

Key words: Chinese medicine; endothelial cells; neutrophils (PMNs); lysozyme

CLC Number:

S853.9

ZHU Wenyu, WANG Yun, SHI Xiaojie, FENG Bo, DONG Hong, WANG Lijuan, MU Xiang. Effects of Different Chinese Herbs on the Lysozyme of Transendothelial Migration of Neutrophil[J]. , 2018, 45(1): 242-248.

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Effects of Different Chinese Herbs on the Lysozyme of Transendothelial Migration of Neutrophil

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