一株鼠伤寒沙门氏菌噬菌体的分离鉴定及对小鼠的防制效果研究

doi:10.16431/j.cnki.1671-7236.2018.01.030

Abstract

Abstract:

This study was aimed to separate a lytic phage against Salmonella Typhimurium from the manure of chicken using double-layer agar culture method. The lytic phage was assessed according to electron microscope observation, thermal stability, pH stability, optimal multiplicity of infection (MOI), One-step growth curve and the inactivation effects on the growth of Salmonella Typhimurium were assayed. As a result, a lytic phage (φBLCC8-0050-3) against Salmonella Typhimurium was successfully isolated from the manure of chicken.φBLCC8-0050-3 had an icosahedral head approximately 65 nm in diameter and had no tail. φBLCC8-0050-3 belonged to family Tectiviridae according to the international classification standard. The plaques of φBLCC8-0050-3 were round and had halo ring around and their sizes which was about 2 to 3 mm in diameter. φBLCC8-0050-3 was stable over a range of temperature (30 to 50℃) and pH (3 to 10). The optimal MOI of φBLCC8-0050-3 was 0.0001. One-step growth experiment showed that the latent time and burst time were 20 and 180 min, respectively, and the burst size was 43 333 PFU/cell. From the control effect of bacteriophage against Salmonella Typhimurium, after 14 days of treatment, the survival rate of mice in the positive control group was 0, was 90% in phage prevention group, and was 70% in treatment group. Compared with the positive control group, the prevention group and the treatment group had a certain effect on mice against Salmonella infection. The results showed that the φ BLCC8-0050-3 was a promising phage, which could be used as a biological antibacterial agent in the prevention and control of Salmonella Typhimurium.

Key words: Salmonella Typhimurium; lytic phages; biological properties; control effect

CLC Number:

S852.61

ZHAO Ying, LIU Xiuxia, LI Jinmin, XU Haiyan, GU Wei. Isolation and Identification of a Phage Against Salmonella Typhimurium and Its Control Effect on Mice[J]. , 2018, 45(1): 234-241.

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Isolation and Identification of a Phage Against Salmonella Typhimurium and Its Control Effect on Mice

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