产肠毒素大肠杆菌K88诱导仔猪炎症反应的分子机制

doi:10.16431/j.cnki.1671-7236.2017.01.037

Abstract

Abstract:

To investigate the molecular mechanism of the inflammatory response in the piglets infected with enterotoxigenic E. coli (ETEC) K88, piglets were infected with ETEC K88,the IL-8 content in serum of piglets were assayed by ELISA,and the mRNA relative expression levels of TLR2/4 and its signal transduction pathway related genes (MyD88,Tollip and Bcl3) in mesenteric lymph nodes were detected by quantitative Real-time PCR. The results showed that compared with control group,the content of IL-8 in serum and the expressions of TLR2/4 in lymph nodes were all extremely significantly or significantly increased at 6 and 24 h after infection (P<0.01;P<0.05),and the IL-8 content and TLR2/4 mRNA expression at 24 h after infection were all significantly lower than those at 6 h after infection (P<0.05).In addition,the expressions of MyD88,Tollip and Bcl3 in lymph nodes were all extremely significantly increased at 24 h after infection compared with control group (P<0.01), but there was no significant difference between experimental group and control group at 6 h after infection (P>0.05). In conclusion,ETEC K88 infected piglets might produce inflammatory cytokines IL-8 through the TLR2/4-MyD88 signaling pathway,which could promote the inflammatory reaction in piglets. This inflammatory response might be regulated by Tollip and Bcl3,which could weak the inflammatory intensity in piglets.

Key words: E. coli K88; piglet; inflammatory response; Toll-like receptors; inflammatory regulation

CLC Number:

S858.28

LI Hai-hua, ZHU Qi, WANG Shi-qiong, YANG Chun-lei, ZHAO Xiang-hua, QIAO Jia-yun, WANG Wen-jie. Molecular Mechanism of the Inflammatory Response Induced by Enterotrxigenic E. coli K88 in Piglets[J]. , 2017, 44(1): 262-267.

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Molecular Mechanism of the Inflammatory Response Induced by Enterotrxigenic E. coli K88 in Piglets

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