大片形吸虫免疫胶体金检测试纸条的研制及初步应用

doi:10.16431/j.cnki.1671-7236.2017.01.036

Abstract

Abstract:

To establish a rapid, simple, sensitive and adapting field application assay for water buffalo Fasciola gigantica, the monoclonal antibodies of hybridoma cell strains 7D1 and 7D4 against excretory-secretary antigen(ES Ag) of Fasciola gigantica were prepared and purified. Meanwhile, the colloidal gold particle was prepared by the sodium cirrate reduction method, then the affinity purified 7D1 monoclone antibodies was labeled with the 30 nm colloidal gold particles. The 7D4 monoclonal antibodies and the goat anti-mouse IgG antibody using as secondary antibodies were coated on the surface of nitrocellulose filter(NC) membrane as the test line (T line) and control line (C line), respectively. The immune colloidal gold test strip was developed by optimizing the experiment conditions. The test results showed the prepared strip had a detecting prescribed minimum of 0.94 μg/mL of Fasciola gigantica excretory-secretary antigen, and it had no any cross reaction with the antigens of Eurytrema pancreaticum, Paramphistome, Chlamydia, Toxoplasma gondii and Trypanosoma evansi. Using the prepared strip to investigate the 334 fresh feces from buffaloes in Guangxi and the positive rate was 38.62%. These results indicated that this strip method was simple, rapid and easy determination, good specificity, high sensitivity, and adapted field application assay for Fasciola gigantica.

Key words: Fasciola gigantica; monoclonal antibody; colloidal gold; test strip

CLC Number:

S855.9

WANG Hua-jun, LIU Yu, JIANG Wei, ZHAO Xue-li, CAO Wei-wei, CHEN Han-zhong, YAN Ruo-qian. Preparation and Primary Application of Immunity Colloidal Gold Test Strip for Fasciola gigantica[J]. , 2017, 44(1): 254-261.

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Preparation and Primary Application of Immunity Colloidal Gold Test Strip for Fasciola gigantica

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