禽致病性大肠杆菌的分离、O血清群鉴定及药物敏感试验

doi:10.16431/j.cnki.1671-7236.2016.12.038

Abstract

Abstract:

One hundred and ninety four avian pathogenic Escherichia.coli (APEC) isolated from different pathotypes of chickens in Hebei province,Shandong province,Henan province,Jiangsu province,Shanxi province and Liaoning province in China between May 2005 and March 2016 were investigated by determination of biochemical characters,O serogroup identification and drug sensitivity test.Out of 194 isolates, 124 strains(63.9%) could be grouped to serogroups O1(3),O2(26),O57(15),O65(47) and O78(33).The drug sensitivity test used eight antibiotics (ampicillin, neomycin, doxycycline, sulfamonomethoxine,tilmicosin,ciprofloxacin ceftiofur and florfenicol) by one strain of O1,O2,O57,O65,O78 serogroup,respectively.The results showed that all strains were sensitive to ciprofloxacin and neomycin, and resistance to other drugs at varying degrees.This study demonstrated that the high prevalence of O65,O78,O2 and O57 isolates existed and provided the basis for the development of multivalent bacterin in future.

Key words: avian pathogenic E.coli; isolation; identification; O serogroups; drug sensitivity test

CLC Number:

S858.3

MA Xing-shu, ZHU Mei-xia, SHI Hu, YANG Guang-ping. Isolation,O Serogrouping and Drug Sensitivity Test of Avian Pathogenic Escherichia coli[J]. , 2016, 43(12): 3343-3348.

References

[1] 马兴树,范翠蝶,夏玉龙.禽致病性大肠杆菌研究进展[J].中国畜牧兽医,2013,40(2):169-173.
[2] 马兴树.禽传染病试验诊断技术[M].北京:化学工业出版社,2006.
[3] Dho-Moulin M,Fairbrother J M.Avian pathogenic Escherichia coli(APEC)[J].Vet Res,1999,30(2-3):299-316.
[4] DebRoy C,Roberts E,Fratamico P M.Detection of O antigens in Escherichia coli[J].Animal Health Research Reviews,2011,12(2):169-185.
[5] 马兴树,朱美霞,王斌,等.河北省南部鸡肝周炎大肠杆菌的分离及血清群鉴定[J].中国农学通报,2009,25(1):12-16.
[6] Dozois C M,Fairbrother J M,Harel J,et al.pap- and pil-related DNA sequences and other virulence determinants associated with Escherichia coli isolated from septicemic chickens and turkeys[J].Infect Immun,1992,60(7):2648-2656.
[7] Blanco J E,Blanco M,Azucena M,et al.Serotypes of Escherichia coli isolated from septicaemic chickens in Galicia(Northwest Spain)[J].Vet Microbiol,1998,61(3):229-235.
[8] Ron R Z.Host specificity of septicemic Escherichia coli:Human and avian pathogens[J].Current Opinions in Microbiology,2006,9(1):28-32.
[9] Deb J R,Harry E G.Laboratory trials with inactivated vaccines against Escherichia coli(O78:K80) infection in fowls[J].Res Vet Sci,1976,20(2):131-138.
[10] Deb J R,Harry E G.Laboratory trials with inactivated vaccines against Escherichia coli (O2:K1) infection in fowls[J].Res Vet Sci,1978,24(3):308-313.
[11] Panigraphy B,Gyimah J E,Hall C F,et al.Immunogenic potency of an oil-emulsified Escherichia coli bacterin[J].Avi Dis,1984,28(2):475-481.
[12] Nakamura K,Cook J K,Frazier J A,et al.Escherichia coli multiplication and lesions in the respiratory tract of chickens inoculated with infectious bronchitis virus and/or Escherichia coli[J].Avi Dis,1992,36(4):881-890.
[13] Nakamura K,Ueda H,Tanimura T,et al.Effect of mixed live vaccine (newcastle disease and infectious bronchitis)and Mycoplasma gallisepticum on the chicken respiratory tract and on Escherichia coli infection[J].J Comp Pathol,1994,111(1):33-42.
[14] Smith H W,Cook J K,Parsell Z E.The experimental infection of chickens with mixtures of infectious bronchitis virus and Escherichia coli[J].J Gen Virol,1985,66(4):777-786.
[15] Wooley R E,Spears K R,Brown J,et al.Relationship of complement resistance and selected virulence factors in pathogenic avian Escherichia coli[J].Avi Dis,1992,36(3):679-684.
[16] Peighambari M,Vaillancourt J P,Wilson R A,et al.Characteristics of Escherichia coli isolates from avian cellulitis[J].Avi Dis,1995,39(1):116-124.
[17] Nagi M S,Raggi L G.Importance to "airsac" disease of water supplies contaminated with pathogenic Escherichia coli[J].AviDis,1972,16(4):718-723.
[18] Chulasiri M,Suthienkul O.Antimicrobial resistance of Escherichia coli isolated from chickens[J].Vet Microbiol,1989,21(21):189-194.
[19] Lafont J P,Bree A,Plat M.Bacterial conjugation in the digestive tracts of gnotoxenic chickens[J].Appl Environ Microbiol,1984,47(4):639-642.
[20] Vidotto M C,Muller E E,deFreitas G C,etal.Virulence factors of avian Escherihia coli[J].Avi Dis,1990,34(3):531-538.

Isolation,O Serogrouping and Drug Sensitivity Test of Avian Pathogenic Escherichia coli

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