贵州省猪源沙门氏菌对β-内酰胺类药耐药性及耐药基因分析

doi:10.16431/j.cnki.1671-7236.2016.07.012

Abstract

Abstract:

In order to analyse the resistance to β-lactams antimicrobial agents and the prevalence of resistant genes of Salmonella in Guizhou province,130 Salmonella strains were isolated and identified from 9 different regions of scale pig farms.The drug sensitivity to 8 kinds of β-lactams antimicrobial agents were determined by using the broth microdilution method.All β-lactams resistant isolates were detected for the presences of TEM,OXA,CTX-M and SHV genes by PCR.The results showed that drug resistance of Salmonella to the commonly used β-lactams antimicrobial agents was very serious,and the resistance rate to ceftazidime was the highest (100%),followed by ampicillin and amoxicillin,were 76.15% and 80.77%,respectively.The resistance rates of ceftiofur and cephalexin were the lowest (46.15%).Salmonella strains were all of multiple drug resistance,of which double resistance was at lowest (2.31%),and eightfold resistance was highest (4.62%),multidrug resistance mainly concentrated in fourfold to sevenfold,accounted for 88.46%.PCR results showed that TEM,OXA,CTX-M genes detection rate were 85%,75% and 46%,respectively,while the SHV gene was not inspected.Resistant phenotype was basically consistent with resistant genes.The results indicated that the resistance of Salmonella stains from pig to β-lactams antimicrobial agents were widespread,and ceftazidime was particularly serious.The TEM,OXA and CTX-M genes were mainly carried β-lactams resistant genes in Salmonella isolates from Guizhou province.It had a great relationship between the prevalence of resistance genes and growth of antimicrobial resistance.

Key words: Salmonella; β-lactamas drugs; drug sensitivity test; resistance genes; resistant phenotypes

CLC Number:

R378.2⁺2

CAO Zheng-hua, TAN Ai-juan, LV Shi-ming, WANG Xiong, DU Guo-qin. Analysis of Drug Resistance and Resistant Genes of Salmonella to β-lactams Antimicrobial Agents Isolated from Pigs in Guizhou Province[J]. , 2016, 43(7): 1737-1742.

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Analysis of Drug Resistance and Resistant Genes of Salmonella to β-lactams Antimicrobial Agents Isolated from Pigs in Guizhou Province

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