京海黄鸡卵巢转录组研究:基因结构分析与新基因发掘注释

doi:10.16431/j.cnki.1671-7236.2016.04.002

Abstract

Abstract: In the present study, in order to improve part of the gene structure and mine new genes, transcriptome of ovary of 4 low and 4 high egg production Jinghai Yellow chickens were analyzed using RNA-Seq technology.The sequencing data was analyzed by bioinformatics methods.After quality control, 484 992 074 clean reads with a total of 61.09 Gb were obtained from the ovary library.1 445 264 SNPs were scanned in which 916 108 SNPs were located in gene region and 552 168 were located in intergenic region.The average new alternative splicing of 8 samples were 12 883. 7 481 genes were optimized gene structure.4 431 novel genes were mined by comparing to the chicken reference genome.1 809 novel genes were obtained by sequence alignment in different database after function annotations.The current study detected the structure of genes in ovary of Jinghai Yellow chicken using RNA-Seq technology, which improved the gene structure information of Jinghai Yellow chicken and provided basis for mining new genes at the molecular level.

Key words: RNA-Seq; Jinghai Yellow chicken; gene structure; SNP; new genes

CLC Number:

HAN Kun-peng, DUAN Lian, LI Ting-ting, WANG Jing-yu, ZHANG Tao, LI Guo-hui, ZHANG Gen-xi, XUE Qian. Study on RNA-Seq of Jinghai Yellow Chicken Ovary:Analysis of Gene Structure and Mining New Genes[J]. , 2016, 43(4): 854-861.

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Study on RNA-Seq of Jinghai Yellow Chicken Ovary:Analysis of Gene Structure and Mining New Genes

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