喷雾干燥细菌素的生物学活性研究

doi:10.16431/j.cnki.1671-7236.2015.12.023

Abstract

Abstract: The standard curve of two antibiotics (colistin sulfate and bacitracin zinc) was carried out to assess antimicrobial activity of bacteriocin,which obtained from our earlier work,using cup-dish method.In addition,we also observed the effects of the reaction temperature on the antibacterial activity of the bacteriocin.Results showed that 125 mg/mL bacteriocin displayed similar antibacterial activity to 26 mg/mL colistin sulfate or 11.9 mg/mL bacitracin zinc.Furthermore,the bacteriocin remained good thermal stability under high temperature (nearly 60 to 80 ℃) in 15 minutes.Overall,this research built a direct relationship between this bacteriocin and antibiotics,and testified good stablility of this bacteriocin,providing theoretical support and technology reference to promote its large scale production.

Key words: bacteriocin; antibiotics; antibacterial activity; thermal stability

CLC Number:

S816.7

WEI Xu-biao, WU Ru-juan, ZHENG Zhao-jun, MA Guang, LIAO Xiu-dong, ZHANG Ri-jun. Study on the Biological Activity of a Spray Dried Bacteriocin[J]. , 2015, 42(12): 3268-3273.

References

[1] Galvin M,Hill C,Ross R P.Lacticin 3147 displays activity in buffer against gram-positive bacterial pathogens which appear insensitive in standard plate assays[J].Lett Appl Microbiol,1999,28(5):355-358.
[2] Goldstein B P,Wei J,Greenberg K,et al.Activity of nisin against Streptococcus pneumoniae,in vitro,and in a mouse infection model[J].J Antimicrob Chemother,1998,42(2):277-278.
[3] 胡婷,陆文清.枯草芽孢杆菌MA139类细菌素抑菌活性的研究[J].中国畜牧杂志,2012,48(3):56-59.
[4] 李振森.解淀粉芽孢杆菌Y-32-1产细菌素的研究[D].青岛:青岛科技大学,2014.
[5] Mendo S,Faustino N A,Sarmento A C,et al.Purification and characterization of a new peptide antibiotic produced by a thermotolerant Bacillus licheniformis strain[J].Biotechnol Lett,2004,26(2):115-119.
[6] Jack R W,Tagg J R,Ray B.Bacteriocins of gram-positive bacteria[J].Microbiol Rev,1995,59(2):171-200.
[7] 尚雅婧,张日俊.细菌素应用的理论与实践[J].中国畜牧兽医,2010,37(5):26-30.
[8] 袁军.加工工艺及参数对饲料加工质量的影响[D].大连:大连海洋大学,2014.
[9] 王庆伟.细菌素Sublancin对金黄色葡萄球菌的抑制作用及其机制的研究[D].北京:中国农业大学,2014.
[10] 栾超.利用SUMO融合技术在枯草芽孢杆菌中重组表达抗菌肽cathelicidin-BF及其生物学活性研究[D].杭州:浙江大学,2014.
[11] 张日俊,谢建华,李桂冠.枯草芽孢杆菌LFB112、其产生的细菌素及应用[P].专利编号:201010560828.
[12] 陈香,谯仕彦,李波,等.添加天蚕素抗菌肽对断奶仔猪生长性能的影响[J].中国饲料,2011,12:25-26.
[13] 任建波,毛宗林,张立彬.天蚕素抗菌肽替代杆菌肽锌对断奶仔猪生产性能及腹泻的影响[J].中国畜牧杂志,2013,49(14):59-61.
[14] 郭云,孟祥晨.喷雾干燥对发酵乳杆菌KLDS1.0709存活影响的研究[J].食品工业科技,2011,32(2):109-111.
[15] Gardiner G E,O'Sullivan E,Kelly J,et al.Comparative survival rates of human-derived probiotic Lactobacillus paracasei and L.salivarius strains during heat treatment and spray drying[J].Appl Environ Microbiol,2000,66(6):2605-2612.
[16] 区伟佳,王荣,李华兴,等.高浓度大豆生防芽孢杆菌粉剂喷雾干燥微胶囊化工艺研究[J].大豆科技,2010,3:3-6.
[17] 胡彦茹,何余湧,陆伟,等.不同调质温度对肉鸡颗粒饲料加工质量的影响[J].饲料工业,2011,32(23):34-36.
[18] 苏东海,刘萍,郑亚安,等.包被工艺条件对植酸酶热稳定性的影响[J].生物加工过程,2004,2(3):40-45.
[19] 张渊舒,罗琴,攸德伟.植酸酶颗粒剂生产工艺的研究[J].江西农业学报,2011,23(5):124-125.
[20] 徐淑玲.加工工艺对饲料中热敏物质的影响及后添加工艺[J].广西畜牧兽医.2007(5):237-239.

Study on the Biological Activity of a Spray Dried Bacteriocin

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Wu, WANG Bi, BAI Yu, CHEN Qirong. Research Progress on Antibacterial Activity of Modified Lysozyme [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 942-951.
[2]	YIN Yangyan, PAN Yan, LI Xiaoning, LI Jun, LIAO Yuying, LI Changting, WEI Tianchao, PENG Hao. Study on Biological Characteristics and Antibacterial Activity in vitro of a Lactobacillus plantarum Strain [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 666-673.
[3]	MA Xiaojiao, ZHAO Yankun, SHAO Wei, WU Yating, LI Ming, LIU Huimin, MENG Lu, CHEN He. Isolation,Identification and Drug Resistance Analysis of Staphylococcus aureus in Raw Milk in Some Areas of Xinjiang [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 789-797.
[4]	LIU Baoling, CHU Pinpin, LI Chunling, YANG Dongxia, JIANG Zhiyong, ZHANG Kunli, SONG Shuai, GOU Hongchao, CAI Rujian. Analysis of Resistance and Resistance Genes of Streptococcus suis Clinical Isolates to Tetracyclines [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2796-2804.
[5]	ZHANG Yu, MA Xi, ZHAO Jinbiao, LU Lin. Optimization of Fermentation Conditions and Determination of Antibacterial Activity of Bacillus licheniformis [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2812-2819.
[6]	SUN Yan, FENG Xiaowei, LIU Jiawei, LIU Bei, JIN Tianxiong, FENG Mingque, LIU Mingchao. Isolation,Identification and Antibacterial Activity of Lactic Acid Bacteria from Reproductive Tract of Healthy Dairy Cows [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1852-1859.
[7]	LIU Jinhuan, ZHANG Shanling, SONG Wei, LUO Wanhe, CHEN Wei. Preparation of Quercetin-tilmicosin Mixture Nanoparticles and Its Antibacterial Activity Against Small Colony Variant Strains of Staphylococcus aureus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1470-1478.
[8]	GUO Jinxiang, LYU Huiru, LI Jiaofeng, LIAO Chengshui, ZHANG Linlin, NIU Junhui. Antibacterial Activity of Licochalcone A Against Pseudomonas aeruginosa Isolated from Mink [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1181-1188.
[9]	LI Weihao, SUN Yuanyuan, LIU Juxiang. Preparation of Oregano Oil Macleaya cordata Oral Solution and Its Antibacterial Activity in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 718-730.
[10]	GUO Bin, SHI Han, FENG Haibo, LIU Qun. Study on in vitro Antibacterial and Antiviral Activity of Compound Shuanghuanglian Preparation [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4832-4842.
[11]	YANG Yanwen, LI Zimi, LIU Qun. Study on Antibacterial and Antiviral Activity of Different Components of Shuanghuanglian Soluble Powder [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 3087-3096.
[12]	ZHANG Qingjuan, MA Xuanxuan, TENG Da, YANG Na, WANG Xiumin, MAO Ruoyu, HAO Ya, SHAN Yuxue, LIU He, FAN Huan, WANG Jianhua. Study on Antibacterial Effect of Antimicrobial Peptide NZ2114 Against Streptococcus dysgalactiae Isolated from Bovine Mastitis [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2603-2614.
[13]	XIE Zongxiu, LI Shangwei, ZHOU Deshun, SONG Xiangdong, JIA Ning, LIANG Jianping. Study on Essential Oil Composition Analysis and Antibacterial Activity from Dicranostigma leptopodum (Maxim.) Fedde [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1611-1617.
[14]	YAN Wei, YANG Na, MAO Ruoyu, WANG Xiumin, HAO Ya, MA Xuanxuan, TENG Da, WANG Jianhua. Recombinant Expression and Activity Analysis of Phage Lyase Lysep3 [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(4): 1018-1027.
[15]	FENG Junsen, XIONG Yunxia, WU Qiwen, WANG Li, JIANG Zongyong, YI Hongbo. The Effect of Lactobacillus reuteri LR1 on the Immune Response of Swine Common Virus Vaccine [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(3): 884-891.