日粮添加罗伊氏乳杆菌LR1对猪常见病毒疫苗免疫效果的影响

doi:10.16431/j.cnki.1671-7236.2020.03.028

Abstract

Abstract: In order to study the effect of Lactobacillus reuteri LR1 isolated earlier on immunological effect of swine common viral vaccines,the whole process of Lactobacillus reuteri LR1 was used instead of feed antibiotics.A total of 144 weaned pigs (21 days old,Duroc×Landrace×Large White) were selected and randomly divided into 3 groups with 8 replicates in each group and 6 pigs in each replicate.The control group (CON) was fed basic diet,and the antibiotic group (OA) was fed basic diet +100 mg/kg olaquindox +75 mg/kg aureomycin,and the probiotics group (LR1) was fed basic diet +5×10¹⁰ CFU/kg Lactobacillus reuteri LR1.The use of antibiotics was stopped at 120 d,and the experimental period was 166 d.All pigs were immunized according to the unified immunization procedure.The antibody levels of porcine reproductive and respiratory syndrome virus (PRRSV),Hog cholera virus (HCV),foot-and-mouth disease virus (FMDV),pseudorabies virus (PRV),porcine parvovirus (PPV) and the level of IgG were detected in the serum of pigs at the 14th,42nd,120th,127th,134th and 166th days after the test.The results showed that,compared with the control group and the antibiotic group,the antibody level of FMDV in the serum of probiotics group on the 14th day was significantly increased (P<0.05).Compared with the control group,the antibody level of PPV in the serum of probiotics group and antibiotic group on the 120th day were significantly increased (P<0.05).The antibody level of PRRSV in the serum of the probiotics group on the 166th day was significantly higher than that of the control group (P<0.05).The antibody level of HCV in the serum of the probiotics group on the 166th day was significantly higher than those of the control group and the antibiotic group (P<0.05).In conclusion,dietary Lactobacillus reuteri LR1 supplementation could improve the antibody levels of common viruses in commercial pigs to a certain extent.These data suggested that Lactobacillus reuteri LR1 can be used to develop new substitutes for antibiotic,and has a great potential in the prevention and treatment of diseases during replacing dietary antibiotics.

Key words: Lactobacillus reuteri LR1; antibiotics; pigs; antibody level; IgG

CLC Number:

S816.7

FENG Junsen, XIONG Yunxia, WU Qiwen, WANG Li, JIANG Zongyong, YI Hongbo. The Effect of Lactobacillus reuteri LR1 on the Immune Response of Swine Common Virus Vaccine[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(3): 884-891.

References

[1] ABRIOUEL H,BENOMAR N,COBO A,et al.Characterization of lactic acid bacteria from naturally-fermented Manzanilla alorena green table olives[J].Food Microbiol,2012,32(2):308-316.
[2] 王明艳,朱亚丽,张广智,等.浅谈兽用抗生素应用现状及益生菌的替代潜能[J].现代畜牧兽医,2019,7:36-41. WANG M Y,ZHU Y L,ZHANG G Z,et al.A brief discussion on the application status of veterinary antibiotics and the potential replacement by probiotics[J].Modern Journal of Animal Husbandry and Veterinary Medicine,2019,7:36-41.(in Chinese)
[3] 史自涛,姚焰础,江山,等.粪肠球菌替代抗生素对断奶仔猪生长性能、腹泻率、血液生化指标和免疫器官的影响[J].动物营养学报,2015,27(6):1832-1840. SHI Z T,YAO Y C,JIANG S,et al.Effects of Enterococcus faecalis substitute for antibiotic on growth performance,diarrhea rate,blood biochemical parameters and immune organs of weaner piglets[J].Chinese Journal of Animal Nutrition,2015,27(6):1832-1840.(in Chinese)
[4] 高建忠,黄克和,秦顺义.不同硒源对仔猪组织硒沉积和抗氧化能力的影响[J].南京农业大学学报,2006,1:85-88. GAO J Z,HUANG K H,QIN S Y.Effects of different selenium sources on tissue selenium retention and anti-oxidative activities in weaned piglets[J].Journal of Nanjing Agricultural University,2006,1:85-88.(in Chinese)
[5] 秦顺义,黄克和,高建忠.富硒益生菌对小鼠免疫功能及抗氧化能力的影响[J].营养学报,2006,5:423-426. QIN S Y,HUANG K H,GAO J Z.Effects of selenium-enriched probiotics on immunity and antioxidation function in mice[J].Acta Nutrimenta Sinica,2006,5:423-426.(in Chinese)
[6] 刘虎传,张敏红,冯京海,等.益生菌制剂对早期断奶仔猪生长性能和免疫指标的影响[J].动物营养学报,2012,24(6):1124-1131. LIU H C,ZHANG M H,FENG J H,et al.Effects of probiotic preparation on growth performance and immune indices of early weaner piglets[J].Chinese Journal of Animal Nutrition,2012,24(6):1124-1131.(in Chinese)
[7] HSUEH H Y,YUEH P Y,YU B,et al.Expression of Lactobacillus reuteri Pg4 collagen-binding protein gene in Lactobacillus casei ATCC 393 increases its adhesion ability to Caco-2 cells[J].Journal of Agricultural and Food Chemistry,2010,58(23):12182-12191.
[8] 贾丹,王雅,李禤,等.罗伊氏乳杆菌ZL2a株体外益生潜能的评价[J].中国兽医科学,2019,5:1-8. JIA D,WANG Y,LI X,et al.In vitro evaluation of probiotic potential of Lactobacillus reuteri ZL2a[J].Chinese Veterinary Science,2019,5:1-8.(in Chinese)
[9] LIU Y,FATHEREE N Y,MANGALAT N,et al.Human-derived probiotic Lactobacillus reuteri strains differentially reduce intestinal inflammation[J].American Journal of Physiology-Gastrointestinal and Liver Physiology,2010,299(5):G1087-G1096.
[10] 张如春,司华哲,陈双双,等.罗伊氏乳杆菌在畜禽生产中应用的研究进展[J].动物营养学报,2019,31(3):1031-1036. ZHANG R C,SI H Z,CHEN S S,et al.Research progress on application of Lactobacillus reuteri in livestock husbandry[J].Chinese Journal of Animal Nutrition,2019,31(3):1031-1036.(in Chinese)
[11] CASAS I A,DOBROGOSZ W J.Validation of the probiotic concept:Lactobacillus reuteri confers broad-spectrum protection against disease in humans and animals[J].Microbial Ecology in Health and Disease,2000,12(4):247-285.
[12] WANG Z,WANG L,CHEN Z,et al.In vitro evaluation of swine-derived Lactobacillus reuteri:Probiotic properties and effects on intestinal porcine epithelial cells challenged with enterotoxigenic Escherichia coli K88[J].Journal of Microbiology and Biotechnology,2016,26(6):1018-1025.
[13] 杨广达,王丽,易宏波,等.罗伊氏乳杆菌LR1对断奶仔猪血清生化指标和肠道营养物质转运载体mRNA表达的影响[J].动物营养学报,2018,30(11):4589-4600. YANG G D,WANG L,YI H B,et al.Effects of Lactobacillus reuteri LR1 on serum biochemical indexes and mRNA expressions of intestinal nutrient transporters of weaned piglets[J].Chinese Journal of Animal Nutrition,2018,30(11):4589-4600.(in Chinese)
[14] YI H,WANG L,XIONG Y,et al.Effects of Lactobacillus reuteri LR1 on the growth performance,intestinal morphology,and intestinal barrier function in weaned pigs[J].Journal of Animal Science,2018,6:2343-2351.
[15] 李海燕.桦褐孔菌发酵物对SPF鸡生长性能和免疫性能的影响[D].邯郸:河北工程大学,2018. LI H Y.Effect of Inonotus obliquus fermentation products on growth and immune performance in SPF chicken[D].Handan:Hebei University of Engineering,2018.(in Chinese)
[16] AI W,YUE Y,XIONG S,et al.Enhanced protection against pulmonary Mycobacterial challenge by chitosan-formulated polyepitope gene vaccine is associated with increased pulmonary secretory IgA and gamma-interferon T cell responses[J].Microbiology and Immunology,2013,57(3):224-235.
[17] 刘伟杰.CpG ODN对鸭源新城疫病毒流行株(SD03)灭活苗免疫增强效应研究[D].泰安:山东农业大学,2016. LIU W J.Immunopotentiation efficiency of CpG ODN on epidemic strain (SD03) inactivated vaccine of duck-origin Newcastle disease virus[D].Tai'an:Shandong Agricultural University,2016.(in Chinese)
[18] 侯成立.罗伊氏乳杆菌全基因组序列分析及其调节仔猪肠黏膜免疫功能的研究[D].北京:中国农业大学,2015. HOU C L.Whole genome sequence analysis of Lactobacillus reuteri and its regulation in intestinal mucosal immunity of piglets[D].Beijing:China Agricultural University,2015.(in Chinese)
[19] VILLENA J,SALVA S,AGUERO G,et al.Immunomodulatory and protective effect of probiotic Lactobacillus casei against Candida albicans infection in malnourished mice[J].Microbiology and Immunology,2011,55(6):434-445.
[20] WANG Z L,WANG L,CHEN Z,et al.In vitro evaluation of swine-derived Lactobacillus reuteri:Probiotic properties and effects on intestinal porcine epithelial cells challenged with enterotoxigenic Escherichia coli K88[J].Journal of Microbiology and Biotechnology,2016,26(6):1018-1025.
[21] HOU C L,ZENG X F,YANG F J,et al.Study and use of the probiotic Lactobacillus reuteri in pigs:A review[J].Journal of Animal Science and Biotechnology,2015,6:14.
[22] 张紫薇.罗伊氏乳杆菌J1益生特性初步评价及基因组学研究[D].哈尔滨:东北农业大学,2019. ZHANG Z W.Study on the probiotic propertise and complete genome sequence of Lactobacillus reuteri J1[D].Haerbin:Northeast Agricultural University,2019.(in Chinese)
[23] 李琪,刘启宏,冀池海,等.微生态制剂对猪流行性腹泻和伪狂犬病疫苗免疫效果的影响[J].畜牧与兽医,2017,12:120-123. LI Q,LIU Q H,JI C H,et al.Effect of a microecological agent on the immunization on against porcine epidemic diarrhea and pseudorabies vaccines[J].Animal Husbandry and Veterinary Medicine,2017,12:120-123.(in Chinese)
[24] WANG X,YANG F,LIU C,et al.Dietary supplementation with the probiotic Lactobacillus fermentum I5007 and the antibiotic aureomycin differentially affects the small intestinal proteomes of weanling piglets[J].The Journal of Nutrition,2012,142(1):7-13.

The Effect of Lactobacillus reuteri LR1 on the Immune Response of Swine Common Virus Vaccine

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WEI Mingbang, BIANBA Qiongda, XIAO Qingqing, DUAN Mengqi, CHAMBA Yangzom, SHANG Peng. Cloning,Bioinformatics and Expression Analysis of CDKN1B Gene in Tibetan Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2196-2206.
[2]	ZHANG Fangwei, ZHANG Qi, LEI Liangliang, SUN Wusheng, ZHANG Di, ZHANG Yunpeng, ZHANG Jingbo, WANG Xiuquan, ZHANG Jing, ZHANG Shumin. Polymorphism of HBEGF Gene and Its Association with Reproductive Traits in Songliao Black Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2370-2379.
[3]	PAN Pengcheng, LEI Zongquan, LI Xian, HU Xiangyun, QIN Qiantao, QIN Zhaoxian, GUAN Zhihui, CHEN Baojian, XIE Bingkun. Bioinformatics Analysis,Eukaryotic Expression Vector Construction and Tissue Expression of MYL2 Gene in Luchuan Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1796-1806.
[4]	LIU Hongrun, ZHU Siran, FENG Lingli, ZHANG Kun, YAN Gang, WANG Yubin, ZHANG Shuai, JIANG Shan, XU Di, LAN Ganqiu, LIANG Jing. Cloning,Bioinformatics Analysis and Tissue Expression Localization of CDO1 Gene in Large White Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1352-1363.
[5]	CHANG Yitong, ZHANG Wei, PENG Yinglin, CHEN Chen. Evolutionary Analysis,Target Gene Prediction and Tissue Expression Analysis of miR-192 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 882-892.
[6]	MA Xiaojiao, ZHAO Yankun, SHAO Wei, WU Yating, LI Ming, LIU Huimin, MENG Lu, CHEN He. Isolation,Identification and Drug Resistance Analysis of Staphylococcus aureus in Raw Milk in Some Areas of Xinjiang [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 789-797.
[7]	YIN Yi, LYU Yanqiu, CHEN Xuan, CAO Lipeng, ZHANG Junzheng, JIN Yi. Effects of Capacitation on Sperm Quality and Regulation of Acrosin Inhibitor Levels by Ubiquitin in Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 174-185.
[8]	XIAO Li, NIU Xiaojie, LIU Qingqing, WANG Yueli, CHEN Chuangfu, YI Jihai. Construction of Recombinant Adenovirus with VP4 Gene of Porcine Rotavirus and Evaluation of Antibody Level [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 260-269.
[9]	DONG Wanyu, ZHANG Jumin, BIAN Sushu, ZHOU Yingshan, DU Jing, YU Haoran, ZHONG Wei, SONG Houhui, WANG Xiaodu. Comparative Analysis of Environmental Factors in Fattening House Under Two Ventilation Modes in Hangzhou Large-scale Pig Farm [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 421-428.
[10]	CHEN Chuanhe, LIU Jiali, ZHANG Lilan, ZHAO Ying, TAO Cong. Bioinformatics Analysis of Porcine SGK Family Genes and Their Expression in Porcine Adipose Tissues and Adipocytes [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3310-3320.
[11]	YANG Man, LIU Hai, ZHANG Run, HU Ziping, NIU Naiqi, WANG Lixian, ZHANG Longchao. Association Analysis of Polymorphism of MYH3 and MYH13 Genes with Meat Quality Traits in Beijing Black Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3428-3437.
[12]	YU Peng, NIU Xiaoyu, DONG Ling, LU Mengqi, CHEN Yanhong, LI Fan, SONG Hui. Research Progress on the Role of lncRNA in Porcine Abortion-related Virus Infection [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3559-3568.
[13]	NIE Jingru, MA Li, YAN Dawei, DENG Jun, ZHANG Hao, ZHANG Bo, LIU Jinqiao, DONG Xinxing. Analysis of Differentially Expressed Genes and Regulation Pathways of Intramuscular Fat Deposition in Large Diqing Tibetan Pigs at Different Growth Stages [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2855-2868.
[14]	YU Zonggang, MA Haiming. Research Progress on Isolation and Culture of Porcine Skeletal Muscle Satellite Cells in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2931-2942.
[15]	YANG Tao, ZHANG Mingjie, XU Ran, ZHANG Han, ZHANG Mengmeng, WU Keliang. Study on Genetic Evaluation of Reproductive and Growth Traits in Pigs by BLUP Method [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2971-2981.