四川省部分地区山羊沙门氏菌健康带菌率及耐药性调查

doi:10.16431/j.cnki.1671-7236.2015.02.037

Abstract

Abstract: To investigate the prevalence of Salmonella in clinical healthy goats in Sichuan province,a total of 196 fecal samples collected from 5 goat farms in Sichuan province were tested in this study.Each sample was pre-enriched by buffered peptone water (BPW),and then selectively enriched by tetrathionate broth base (TTB).The enrichments were detected by PCR assay targeting the invA gene to determine the presence of Salmonella.The positive samples detected by PCR were isolated and identified; 25 Salmonella isolates were randomly selected to test the antibiotic resistance against 15 antibiotics.The results showed that the positive rate of Salmonella in fecal samples was 54.59%.Antibiotic sensitivity analysis showed that all of the tested strains were sensitive to amikacin,while the strains were resistant to the other 14 antibiotics in certain degrees.The rate of multi-drug resistance was 88%,and among the 25 isolates,52% were resistant to 2 to 7 antibiotic and 36% were resistant to 9 to 13 antibiotics.The results indicated that Salmonella were prevalent among clinical healthy goats in Sichuan province,and the multi-drug resistance of the Salmonella isolates from goats were quite common,which had significant implication for public health.

Key words: goats; Salmonella; prevalence in healthy animal; antibiotic resistance

CLC Number:

S852.61

WANG Jing-jing, XIA Bin-yan, YUE Hua, TANG Cheng. Prevalence and Resistance Analysis of Salmonella in Clinical Healthy Goats in Sichuan Province[J]. , 2015, 42(2): 472-477.

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Prevalence and Resistance Analysis of Salmonella in Clinical Healthy Goats in Sichuan Province

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