蒙脱石和凹凸棒石对霉菌毒素吸附性能的研究

›› 2014, Vol. 41 ›› Issue (11): 133-138.

蒙脱石和凹凸棒石对霉菌毒素吸附性能的研究

梁晓维^1,2, 李发弟¹, 张军民^2,4, 马悦培^2,3, 赵青余^2,4, 高红亮³

1. 甘肃农业大学动物科学技术学院, 甘肃兰州 730070;
2. 中国农业科学院北京畜牧兽医研究所, 农业部华北动物遗传资源与营养科学观测实验站, 北京 100193;
3. 华东师范大学, 上海 200062;
4. 中国农业科学院—世界农用林业中心农用林业与可持续畜牧业联合实验室, 北京 100193

修回日期:2014-09-17 出版日期:2014-11-20 发布日期:2014-12-06
通讯作者: 李发弟, 张军民
作者简介:梁晓维(1988-),女,陕西人,硕士生,研究方向:动物营养与饲料科学。
基金资助:
中央级公益性科研院所基本科研业务费专项基金项目(2009yg-3);北京市大兴区与中国农业科学院科技合作项目。

Study on Adsorption of Montmorillonite and Attapulgite to Mycotoxins

LIANG Xiao-wei^1,2, LI Fa-di¹, ZHANG Jun-min^2,4, MA Yue-pei^2,3, ZHAO Qing-yu^2,4, GAO Hong-liang³

1. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
2. Scientific Observing and Experiment Station of Animal Genetic Resources and Nutrition in North China, Ministry of Agriculture, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
3. East China Normal University, Shanghai 200062, China;
4. CAAS-ICRAF joint Laboratory on Agroforestry and Sustainable Animal Husbandry, Beijing 100193, China

Revised:2014-09-17 Online:2014-11-20 Published:2014-12-06

摘要/Abstract

摘要： 试验将蒙脱石和凹凸棒石分别添加到含有黄曲霉毒素B₁(AFB₁)、玉米赤霉烯酮和T-2毒素的人工胃液或人工肠液中,探讨2种矿物对霉菌毒素的吸附作用。结果表明,蒙脱石和凹凸棒石对AFB₁、玉米赤霉烯酮、T-2毒素的吸附均在1 h达到平衡;钠基蒙脱石对AFB₁和T-2毒素的吸附率高于钙基蒙脱石和凹凸棒石,吸附率分别为96.8%、38%;蒙脱石和凹凸棒石对玉米赤霉烯酮的吸附率均小于10%。pH对蒙脱石和凹凸棒石吸附霉菌毒素影响显著(P<0.05),pH 5时钙基蒙脱石对AFB₁的吸附率显著高于pH 3、pH 7时(P<0.05),为90.1%;蒙脱石和凹凸棒石对玉米赤霉烯酮的吸附效果在pH 3时较佳,对T-2毒素的吸附效果在pH 7时显著高于pH 3、pH 5时(P<0.05)。蒙脱石和凹凸棒石对吸附的霉菌毒素均存在解吸现象,其中AFB₁和玉米赤霉烯酮解吸明显。蒙脱石和凹凸棒石对霉菌毒素的吸附作用使其成为重要的吸附剂,然而作为吸附剂的同时不能忽视霉菌毒素的解吸现象,综合考虑吸附与解吸2种现象以便在生产实践中更好的脱除霉菌毒素。

关键词: 蒙脱石; 凹凸棒石; 黄曲霉毒素B₁; 玉米赤霉烯酮; T-2毒素

Abstract: The aim of this study was to determine the adsorption and desorption of aflatoxin B₁ (AFB₁),zearalenone,T-2 toxin to montmorillonite and attapulgite in the artificial gastric juice and intestinal juice. The results showed that the adsorption equilibrium of AFB₁,zearalenone,T-2 toxin to montmorillonite and attapulgite were established within 1 h. The adsorption rates of AFB₁ and T-2 toxin to sodium montmorillonite were 96.8%,38%,respectively. Zearalenone adsorbed by montmorillonite and attapulgite were less than 10%.The pH of artificial intestinal fluid had a significant influence on adsorbing mycotoxins (P<0.05).The adsorption rate of AFB₁ to sodium montmorillonite was larger at pH 5 than pH 3 and pH 7 (P<0.05),90.1%.High adsorption efficiency of zearalenone to montmorillonite and attapulgite was achieved at pH 3.The adsorption rate of T-2 toxin to montmorillonite and attapulgite was larger at pH 7 than pH 3 and pH 5 (P<0.05).The complex compound formed by montmorillonite or attapulgite with mycotoxins has the desorption phenomena,expecially AFB₁ and zearalenone.Because of the adsorption of AFB₁,zearalenone,T-2 toxin to montmorillonite and attapulgite,they are important adsorbent.However,the desorption phenomena should not be neglected.Selection of montmorillonite or attapulgite as a feed additive should be considered their adsorption and desorption in practice.

Key words: montmorillonite; attapulgite; aflatoxin B₁; zearalenone; T-2 toxin

中图分类号:

S816.7

梁晓维, 李发弟, 张军民, 马悦培, 赵青余, 高红亮. 蒙脱石和凹凸棒石对霉菌毒素吸附性能的研究[J]. , 2014, 41(11): 133-138.

LIANG Xiao-wei, LI Fa-di, ZHANG Jun-min, MA Yue-pei, ZHAO Qing-yu, GAO Hong-liang. Study on Adsorption of Montmorillonite and Attapulgite to Mycotoxins[J]. China Animal Husbandry & Veterinary Medicine, 2014, 41(11): 133-138.

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