白藜芦醇对黄曲霉毒素B1致兔肾脏损伤的保护作用

doi:10.16431/j.cnki.1671-7236.2024.01.016

摘要/Abstract

摘要： 【目的】探究白藜芦醇（resveratrol，Res）对黄曲霉毒素B₁（aflatoxin B₁，AFB₁）致兔肾脏损伤的保护作用。【方法】将21只40日龄的家兔随机分为3组，每组7只，分别为对照组、AFB₁组和AFB₁＋Res组，其中对照组饲喂基础饲粮，AFB₁组饲喂含0.3 mg/kg BW AFB₁的基础饲粮、AFB₁＋Res组饲喂含0.3 mg/kg BW AFB₁和30 mg/kg BW Res的基础饲粮，试验期21 d。试验结束后观察家兔肾脏组织病理变化，检测血清中肌酐（creatinine，CRE）、尿素氮（urea nitrogen，BUN）和尿酸（uric acid，UA）含量，检测肾脏组织中谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）、超氧化物歧化酶（superoxide dismutase，SOD）、总抗氧化能力（total antioxidant capacity，T-AOC）和丙二醛（malonaldehyde，MDA）含量/活性。【结果】与对照组相比，AFB₁组家兔肾脏可见肾小囊腔扩张、肾小管上皮细胞排列紊乱、细胞核模糊和脱落，且肾脏中糖原沉积增多；血清中CRE、BUN和UA含量均显著升高（P<0.05）；肾脏组织中GSH-Px、SOD活性和T-AOC均显著降低（P<0.05），MDA含量显著升高（P<0.05）。与AFB₁组相比，AFB₁+Res组家兔肾脏组织结构损伤得到缓解，且糖原沉积减少；血清中CRE、BUN和UA含量均显著降低（P<0.05）；肾脏组织中GSH-Px、SOD活性和T-AOC均显著升高（P<0.05），MDA含量显著降低（P<0.05）。【结论】饲料中添加Res可以通过提高兔肾脏抗氧化能力、调节氧化应激水平而有效缓解AFB₁引起的兔肾脏损伤。

关键词: 兔; 白藜芦醇; 黄曲霉毒素B₁; 肾脏损伤

Abstract: 【Objective】 This study explored the protective effect of resveratrol (Res) on kidney injury in rabbits induced by aflatoxin B₁ (AFB₁).【Method】 21 rabbits aged 40 days were randomly divided into 3 groups, 7 rabbits in each group, which were control group, AFB₁ group and AFB₁+Res group.The rabbits in control group was fed with basic diet, in AFB₁ group was fed with basal diet containing 0.3 mg/kg BW AFB₁, in AFB₁+Res group was fed with basal diet containing 0.3 mg/kg BW AFB₁ and 30 mg/kg BW Res.The experiment period was 21 days.At the end of the experiment, the pathological changes of kidney tissues in rabbit were observed, and the contents of creatinine (CRE), urea nitrogen (BUN) and uric acid (UA) in serum were detected.And the glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), total antioxidant capacity (T-AOC) and malonaldehyde (MDA) contents/activeties in kidney tissues were evaluated.【Result】 Compared with the control group, the pathological changes in the kidneys of rabbits in AFB₁ group included narrowed capsular space, disordered arrangement of renal tubular epithelial cells, fuzzy and exfoliated nuclei, and glycogen accumulation in the kidneys.The contents of CRE, BUN and UA in serum of rabbits in AFB₁ group were significantly increased (P<0.05).The activities of GSH-Px, SOD and T-AOC in the kidney tissues of rabbits in AFB₁ group were significantly decreased (P<0.05), and the level of MDA was significantly increased (P<0.05).Compared with AFB₁ group, for the rabbits in Res+AFB₁ group, the renal tissue structure damage in kidney was alleviated and the accumulation of glycogen was significantly reduced, the contents of CRE, BUN and UA in serum were significantly decreased, the levels of GSH-Px, SOD and T-AOC in the kidney tissue were significantly increased (P<0.05), and the level of MDA was significantly decreased (P<0.05).【Conclusion】 Adding Res in the diet could effectively alleviate the kidney damage caused by AFB₁ via improving the antioxidant capacity to regulate the level of oxidative stress of kidney in the rabbit.

Key words: rabbit; resveratrol; aflatoxin B₁; kidney injury

中图分类号:

S816.7

刘潍萁, 张伊阳, 张自强, 丁轲, 刘玉梅. 白藜芦醇对黄曲霉毒素B₁致兔肾脏损伤的保护作用[J]. 中国畜牧兽医, 2024, 51(1): 153-159.

LIU Weiqi, ZHANG Yiyang, ZHANG Ziqiang, DING Ke, LIU Yumei. Protective Effect of Resveratrol on Kidney Injury Induced by Aflatoxin B₁ in Rabbits[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(1): 153-159.

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白藜芦醇对黄曲霉毒素B₁致兔肾脏损伤的保护作用

Protective Effect of Resveratrol on Kidney Injury Induced by Aflatoxin B₁ in Rabbits

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