和厚朴酚对镉致鸡肾脏损伤的保护作用

doi:10.16431/j.cnki.1671-7236.2023.03.016

摘要/Abstract

摘要： 【目的】研究和厚朴酚(honokiol,HNK)对镉(Cd)暴露引起动物肾脏损伤的保护作用。【方法】选取48只体重相近(61.05 g±2.00 g)、健康状况良好的1日龄三黄鸡随机分为4组,每组3个重复,每个重复4只。对照组饲喂基础饲粮,Cd组在基础饲粮中添加70 mg/kg氯化镉,HNK组在基础饲粮中添加400 mg/kg HNK,联合处理组(HNK+Cd组)在基础饲粮中添加70 mg/kg氯化镉和400 mg/kg HNK,试验期30 d。【结果】与对照组相比,Cd组鸡肾脏中Cd含量、血清中尿酸(uric acid,UA)及肌酐(creatinine,CREA)含量均极显著升高(P＜0.01),肾脏肾小囊腔隙增大,凋亡细胞数增多,肾组织中硒(Se)、铁(Fe)、锰(Mn)含量均极显著降低(P＜0.01),锌(Zn)、铜(Cu)含量均极显著升高(P＜0.01);HNK组鸡肾脏Zn、Fe含量极显著或显著升高(P＜0.01;P＜0.05),Cd、Se、Cu、Mn含量及血清UA、CREA含量均无显著差异(P＞0.05)。与Cd组相比,HNK+Cd组鸡肾脏Cd、Cu、血清UA含量均显著降低(P＜0.05),CREA含量下降但差异不显著(P＞0.05),肾脏中Se、Zn、Fe、Mn含量显著升高(P＜0.05),肾小管细胞损伤得到缓解、凋亡细胞数量有所减少。【结论】HNK可降低Cd在肾脏中的蓄积,减少肾脏组织凋亡细胞数,并缓解肾脏组织损伤,改善鸡体内微量元素代谢紊乱情况,增强鸡体质。

关键词: 和厚朴酚(HNK); 镉; 鸡; 肾脏损伤; 凋亡; 微量元素

Abstract: 【Objective】 This study was aimed to study the protective effect of honokiol (HNK) on renal injury in animals exposed to cadmium (Cd).【Method】 Forty eight Sanhuang chickens with similar body weight (61.05 g±2.00 g) and good health conditions were randomly divided into four groups,each group had three replicates and each replicate had four chickens.The chickens in control group was fed with basal diet,in Cd group, 70 mg/kg cadmium chloride were added in the basal diet,in HNK group, 400 mg/kg HNK were added in the basal diet, and in the combined treatment group (HNK+Cd group), 70 mg/kg cadmium chloride and 400 mg/kg HNK were added in the basal diet.The feeding period was 30 days.【Result】 Compared with control group,the content of cadmium in the kidney,the serum uric acid (UA) and creatinine (CREA) contents were extremely significantly increased (P＜0.01),the lumen of the renal crypt was enlarged and the number of apoptotic cells was increased,the contents of selenium,iron,and manganese in kidney were extremely significantly decreased (P＜0.01),and the contents of zinc and copper in kidney were extremely significantly increased(P＜0.01) in Cd group.The contents of zinc and iron in kidney were extremely significant or significantly increased (P＜0.01 or P＜0.05),and there were no significant differences in the cadmium,selenium,copper,and manganese contents of kidney,and serum UA and CREA contents in HNK group (P＞0.05).Compared with Cd group,for the chickens in HNK+Cd group,the contents of cadmium,copper in kidney and serum UA were significantly decreased (P＜0.05),the content of CREA was decreased,but the difference was not significant (P＞0.05),the contents of selenium,zinc,iron,and manganese in kidney were significantly increased (P＜0.05),and the renal tubular cell injury was alleviated and the number of apoptotic cells was also reduced.【Conclusion】 HNK could reduce the accumulation of cadmium in kidney,reduce the number of apoptotic cells in kidney tissue,alleviate renal injury,improve the metabolism disorder of trace elements in chicken,and enhance the constitution of chicken.

Key words: honokiol(HNK); cadmium; chicken; renal injury; apoptosis; trace element

中图分类号:

S816.7

黄小倩, 原俊朝, 刘金河, 邹辉, 顾建红, 袁燕, 刘学忠, 刘宗平, 卞建春. 和厚朴酚对镉致鸡肾脏损伤的保护作用[J]. 中国畜牧兽医, 2023, 50(3): 1007-1015.

HUANG Xiaoqian, YUAN Junzhao, LIU Jinhe, ZOU Hui, GU Jianhong, YUAN Yan, LIU Xuezhong, LIU Zongping, BIAN Jianchun. Protective Effect of Honokiol on Cadmium-induced Renal Injury in Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1007-1015.

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