复合生物解毒剂在人工胃肠液中对黄曲霉毒素B1降解率的影响

doi:10.16431/j.cnki.1671-7236.2023.04.011

摘要/Abstract

摘要： 【目的】试验以人工胃肠液(artificial gastrointestinal fluid,AGIF)作为反应体系,研制高效的霉菌毒素生物解毒剂,以达到有效降解黄曲霉毒素B₁(aflatoxin B₁,AFB₁)的目的。【方法】首先考察4种益生菌在人工胃肠液中对AFB₁的降解率,然后使用响应面设计对4种益生菌组合进行优化,制备复合益生菌;其次,将复合益生菌与不同浓度的AFB₁降解酶进行复合,以筛选最佳复合生物解毒剂;最后评估复合生物解毒剂对AFB₁的降解效果。【结果】①枯草芽孢杆菌(Bacillus subtilis)、干酪乳杆菌(Lactobacillus casein)、粪肠球菌(Enterococcus faecalis)和产朊假丝酵母(Candida utilis)在人工胃肠液条件下对AFB₁降解率分别为20.59%、19.97%、28.75%和25.67%;②利用响应面设计对4种益生菌进行优化组合,结果表明4种益生菌配比为1.0×10⁵、1.0×10⁵、1.0×10⁷和1.0×10⁵ CFU/mL时,AFB₁降解率达到最大,为41.5%,显著大于单一益生菌降解率(P＜0.05);③复合益生菌与0.1%的霉菌毒素降解酶配伍合成的复合生物解毒剂,对AFB₁的降解率达到55.28%,显著高于益生菌或霉菌毒素降解酶单独使用的效果(P＜0.05)。【结论】本试验中由益生菌与霉菌毒素降解酶组合形成的复合生物解毒剂可有效降解AFB₁,可为饲料中AFB₁的生物解毒措施提供参考。

关键词: 黄曲霉毒素B₁; 复合益生菌; 霉菌毒素降解酶; 人工胃肠液

Abstract: 【Objective】 In order to improve the degradation rate of aflatoxin B₁ (AFB₁) in animal feed,artificial gastrointestinal fluid (AGIF) was used to make compound biological detoxifier for degrading AFB₁ effectively.【Method】 Firstly,the degradation rates of AFB₁ by four probiotics in AGIF were investigated,then the optimal proportion of 4 species of beneficial microbes was obtained by means of response surface methodology to make compound probiotics for degrading AFB₁.And then the compound probiotics was combined with different concentrations of AFB₁-degrading enzyme to screen the optimal portfolio of compound biological detoxifier for degrading AFB₁ effectively.Finally,effect of compound biological detoxifier on degrading AFB₁ was evaluated under the AGIF condition.【Result】 ①The AFB₁-degradation rates by four species of Bacillus subtilis, Lactobacillus casein,Enterococcus faecalis and Candida utilis were 20.59%,19.97%,28.75% and 25.67%,respectively.② The maximal AFB₁-degradation rate was 41.5% when the visible counts of four probiotics were 1.0×10⁵,1.0×10⁵,1.0×10⁷ and 1.0×10⁵ CFU/mL to make compound probiotics by response surface methodology,significantly higher than that of single probiotic alone (P＜0.05);③When compound probiotics was combined with 0.1% AFB₁-degradation enzyme to make compound biological detoxifier,AFB₁-degradation rate was increased to 55.28%,significantly higher than that of probiotics or AFB₁-degradation enzyme alone (P＜0.05).【Conclusion】 The combination of compound probiotics with mycotoxin-degradation enzyme (compound biological detoxifier) was able to degrade AFB₁effectively.It could provide reference for biological detoxification measures of AFB₁ in feed.

Key words: aflatoxin B₁; compound probiotics; mycotoxin-degradation enzyme; artificial gastrointestinal fluid

中图分类号:

S816.7

郭红伟, 蔡永超, 樊春光, 郝凯旋, 常娟, 王平, 刘超齐, 尹清强. 复合生物解毒剂在人工胃肠液中对黄曲霉毒素B₁降解率的影响[J]. 中国畜牧兽医, 2023, 50(4): 1395-1403.

GUO Hongwei, CAI Yongchao, FAN Chunguang, HAO Kaixuan, CHANG Juan, WANG Ping, LIU Chaoqi, YIN Qingqiang. Effect of Compound Biological Detoxifier on Aflatoxin B₁ Degradation Rate Under Artificial Gastrointestinal Fluid Condition[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1395-1403.

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复合生物解毒剂在人工胃肠液中对黄曲霉毒素B₁降解率的影响

Effect of Compound Biological Detoxifier on Aflatoxin B₁ Degradation Rate Under Artificial Gastrointestinal Fluid Condition

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