›› 2013, Vol. 40 ›› Issue (9): 147-150.

• 生理生化 • 上一篇    下一篇

库拉索芦荟提取物对维氏气单胞菌抑菌作用的研究

杨永立, 王利, 吴满林   

  1. 西南民族大学生命科学与技术学院, 动物遗传育种学国家民委—教育部重点实验室, 四川成都 610041
  • 收稿日期:2013-03-11 出版日期:2013-09-20 发布日期:2013-09-18
  • 通讯作者: 王利 E-mail:qinxin916@yahoo.com.cn
  • 作者简介:杨永立(1992-),女,云南人,学士,研究方向:微生物遗传学。
  • 基金资助:
    四川省科技支撑项目(2013FZ0014);国家级"大学生创新训练计划"项目(201210656018);西南民族大学教育教学改革项目(2012YB01)。

Study on the Bacteriostasis of Aloe Vera Extracts against Aeromonas veronii

YANG Yong-li, WANG Li, WU Man-lin   

  1. Key Laboratory of Animal Genetics and Breeding of State Ethnic Affairs Commission & Ministry of Education, College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China
  • Received:2013-03-11 Online:2013-09-20 Published:2013-09-18

摘要: 采用榨汁法、水煮法和醇提法制备库拉索芦荟提取物,通过K-B法和二倍稀释法测定3种方法芦荟提取物对维氏气单胞菌的抑菌作用。结果显示,用3种方法制备的库拉索芦荟提取物对维氏气单胞菌均有不同程度的抑制作用,榨汁法、水煮法和醇提法得到的库拉索芦荟提取物对3株维氏气单胞菌的平均最小抑菌浓度(minimal inhibitory concentration,MIC)分别为1、0.5和0.125 g/mL,最小杀菌浓度(minimal bactericidal concentration,MBC)分别为1、1和0.25 g/mL。库拉索芦荟提取物对3株维氏气单胞菌均有抑制作用,芦荟提取物浓度越高抑菌效果越明显,其中醇提法的抑菌效率高,水煮法的抑菌作用其次,榨汁法的抑菌效果较弱。

关键词: 库拉索芦荟; 提取物; 维氏气单胞菌; 抑菌

Abstract: To discuss the bacteriostasis of extracts from aloe vera on Aeromonas veronii by juicing method, water extraction and alcohol extraction method, the inhibition activities, the minimal inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of the extracts from three kinds of methods which had significantly antibacterial effect against Aeromonas veronii were determined with K-B programe and the double broth dilution method. The results showed that the extracts from aloe vera with three kinds of methods all displayed sterilization ability on Aeromonas veronii inordinately. MIC of the extracts from aloe vera with juicing method, water extraction and alcohol extraction method were 1, 0.5, 0.125 g/mL, respectively. The MBC were 1, 1, 0.25 g/mL, respectively. Alcohol extract method showed higher activities than the others. But water extraction was considered as the most suitable method for extracting based on both of the antifungal activity and extraction efficiency.

Key words: aloe vera; extractive; Aeromonas veronii; antibacterial effect

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