env基因对J亚群禽白血病病毒体外感染和复制能力的影响

›› 2013, Vol. 40 ›› Issue (9): 143-146.

env基因对J亚群禽白血病病毒体外感染和复制能力的影响

吴晓婵¹, 李娇², 曹伟胜¹, 廖明¹

1. 华南农业大学兽医学院, 广东广州 510642;
2. 广州畜牧科学研究所, 广东广州 510545

收稿日期:2013-02-17 出版日期:2013-09-20 发布日期:2013-09-18
作者简介:吴晓婵(1988-),女,湖南人,硕士生,研究方向:动物传染病。
基金资助:
NSFC-广东联合基金(U0831002);国家肉鸡产业技术体系项目(nycytx-42-G3-03);广东省自然科学基金项目(S2011010001946、10451064201005432)。

Effects of env Gene on Subgroup J Avian Leukosis Virus Infection and Replication

WU Xiao-chan¹, LI Jiao², CAO Wei-sheng¹, LIAO Ming¹

1. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;
2. Guangzhou Institute of Animal Science, Guangzhou 510545, China

Received:2013-02-17 Online:2013-09-20 Published:2013-09-18

摘要/Abstract

摘要： 为探讨env基因对J亚群禽白血病病毒(subgroup J avian leukosis virus,ALV-J)体外感染和复制能力的影响,本研究利用反向遗传方法构建重组病毒,将血管瘤病变型ALV-J HN06株中env元件替换至髓细胞瘤病变型ALV-J NX0101株的相应位置,成功构建了重组质粒pNX-HNenv。重组毒株能在DF-1细胞上稳定增殖,并能被JE9特异性单抗识别,证明获得了具有感染性的重组病毒NX-HNenv株。结果显示,同一亚群内env基因的替换对病毒的体外感染和复制能力无明显影响。

关键词: J亚群禽白血病病毒; env基因; 反向遗传技术; 复制能力

Abstract: In order to rescue the env element-substitution virus, we constructed the recombinant plasmid pNX-HNenv that contained the whole myelomas strain ALV-J NX0101 genome, but with env element of hemangiomas strain ALV-J HN06. The plasmids were transfected into DF-1 cells and the rescued viruses were identified, respectively. The recombinant virus was stable in cell culture during nine passages, and could be identified by ALV-J specific monoclonal antibody JE9 and virus NX-HNenv was rescued. The results showed that the env substitution had no apparent influence on ALV-J virus rescue, virus entry and replication.

Key words: subgroup J avian leukosis virus; env gene; reversegenetics; replication

中图分类号:

Q344⁺.13

吴晓婵, 李娇, 曹伟胜, 廖明. env基因对J亚群禽白血病病毒体外感染和复制能力的影响[J]. , 2013, 40(9): 143-146.

WU Xiao-chan, LI Jiao, CAO Wei-sheng, LIAO Ming. Effects of env Gene on Subgroup J Avian Leukosis Virus Infection and Replication[J]. , 2013, 40(9): 143-146.

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