大河乌猪磷酸酯转移蛋白基因内含子4的SNPs检测及其与生产性能的关联分析

›› 2013, Vol. ›› Issue (7): 143-148.

大河乌猪磷酸酯转移蛋白基因内含子4的SNPs检测及其与生产性能的关联分析

李福泉^1,2, 杨文², 郭斌³, 霍金龙⁴, 史宪伟¹

1. 云南农业大学动物科技学院, 云南省生物多样性与生物技术人才培养基地, 云南昆明 650201;
2. 内江职业技术学院生物技术系, 四川内江 641100;
3. 吉林大学畜牧兽医学院, 吉林长春 130062;
4. 云南农业大学版纳微型猪近交系重点实验室, 云南昆明 650201

收稿日期:2012-12-27 出版日期:2013-07-20 发布日期:2013-07-24
通讯作者: 史宪伟 E-mail:xwshi5@yahoo.com
作者简介:李福泉(1978-),男,山西人,硕士,讲师,研究方向:动物分子遗传育种。
基金资助:
国家自然基金(30270072;30460055);云南省自然科学基金(2002C0064M);2011年四川省"高等教育质量工程"建设,内江职业技术学院畜牧兽医专业综合改革课题子项目(动物防疫与检疫技术精品开放课程)。

SNPs Detection of PLTP-4 among Dahe Blank Porcine and Association Analysis with their Production Performance

LI Fu-quan^1,2, YANG Wen², GUO Bin³, HUO Jin-long⁴, SHI Xian-wei¹

1. Center of Biodiversity and Biotechnology, College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China;
2. Department of Biotechnology, Neijiang Vocational & Tecnical College, Neijiang, 641100, China;
3. College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China;
4. Key Laboratory of Bamma Mini-Pig Inbred Line of Yunan Province, Kunming 650201, China

Received:2012-12-27 Online:2013-07-20 Published:2013-07-24

摘要/Abstract

摘要： 本研究旨在阐明猪磷酸酯转移蛋白(phospholipid transfer protein,PLTP)基因第4内含子多态性及其与生长性状之间的关系。结果表明,该位点具有2种等位基因A/a,F4、F5、F6各群体中的基因频率分别为0.4172/0.5828、0.4155/0.5845、0.4309/0.5691。取不同基因型纯合子样品进行测序,共发现4个单核苷酸多态位点(SNPs),其有3个突变为转换,1个为颠换,多态位点分别为1085、1192、1286和1305 bp。应用最小二乘模型分析不同基因型对生长性状(初生重、45日龄体重、4月龄体重及6月龄体重、体高、体长、管围、背膘厚)的影响。结果显示,F4代各性状基因型间差异不显著(P>0.05);F5代Aa基因型个体初生重及背膘厚显著高于AA基因型(P<0.05),与aa基因型个体间差异不显著(P>0.05);F6代aa基因型个体4月龄体重及Aa基因型个体6月龄管围都极显著高于AA基因型(P<0.01),aa基因型个体6月龄体高显著高于AA基因型(P<0.05),Aa基因型个体初生重显著高于AA基因型(P<0.05)。另外,Aa基因型的雌性个体对初生重及膘厚性状的影响较AA基因型高(P<0.05);Aa基因型雄性个体对管围性状的影响较AA基因型高(P<0.01)。研究结果提示猪PLTP基因Aa基因型对生长性状有着重要的影响,该SNP可能是改良猪经济性状的重要分子标记位点。

关键词: 大河乌猪; 磷酸酯转移蛋白; PCR-RFLP; 生产性能

Abstract: The associations between SNPs in the PLTP gene and production performance of pigs were reported in this study.The results showed alleles detected in the sequence were allele A and a with frequencies of 0.4172 and 0.5828,0.4155 and 0.5845,0.4309 and 0.5691 in F4, F5, F6 generation,respectively. There were 4 mutations of 3 for conversion and 1 for transversion,4 SNPs (1085,1192,1286 and 1305 bp, respectively) were found by the sampling sequence. Associations between the founded Hae Ⅲ RFLP in intron 4 and birth weight, body weight at 45 days, body weight at 4 months, body weight at 6 months, body height at 6 months, body length at 6 months,cannon circumference at 6 months and back-fat thickness at 6 months were tested by least square analysis. In F4 generation,no significant difference between HaeⅢ RFLP and all growth traits(P>0.05). In F5 generation, the birth weight and back-fat thickness at 6 months with Aa genotype were higher than that with AA genotype (P<0.05). In F6 generation,the body weight at 4 months(P<0.01)and body height at 6 months (P<0.05) with aa genotype were higher than that with AA genotype. The cannon circumference at 6 months (P<0.01) and the birth weight (P<0.05) with Aa genotype were higher than that with AA genotype. In addition, the cannon circumference were the highest significant than that with AA genotype male (P<0.01). The birth weight and back-fat thickness at 6 months were higher than that with AA genotype female (P<0.05). These results suggested that the Aa genotype of PLTP gene had some effects on the growth traits of porcine. These SNP might be considered as a potential genetic marker for selection.

Key words: Dahe Blank pigs; phospholipid transfer protein; PCR-RFLP; production performance

中图分类号:

S828.8

李福泉, 杨文, 郭斌, 霍金龙, 史宪伟. 大河乌猪磷酸酯转移蛋白基因内含子4的SNPs检测及其与生产性能的关联分析[J]. , 2013, (7): 143-148.

LI Fu-quan, YANG Wen, GUO Bin, HUO Jin-long, SHI Xian-wei. SNPs Detection of PLTP-4 among Dahe Blank Porcine and Association Analysis with their Production Performance[J]. China Animal Husbandry & Veterinary Medicine, 2013, (7): 143-148.

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