延边黄牛FGF14基因多态性及其对生长性状的影响

doi:10.16431/j.cnki.1671-7236.2020.12.022

摘要/Abstract

摘要： 本研究旨在探索延边黄牛成纤维细胞生长因子14（fibroblast growth factor 14，FGF14）第3外显子多态性及其对生长性状的影响。应用DNA直接测序法对延边黄牛FGF14基因第3外显子进行测序分析，利用高分辨率熔解曲线HRM分型技术对FGF14基因进行分型检测，探究FGF14基因不同基因型与16和24月龄延边黄牛生长性状的关联性，并进行遗传统计学分析。结果显示，在FGF14基因的631 947 bp处检测到1处A>G突变，有2个等位基因：A和G，存在3种基因型：AA、AG和GG。χ²检验结果表明，FGF14基因的基因型频率和等位基因频率在16和24月龄延边黄牛群体中的分布均符合Hardy-Weinberg平衡定律（P>0.05）。多态信息含量（PIC）分析表明，FGF14基因在这2个群体中均处于中度多态（0.25 < PIC < 0.5）。与生长性状关联分析结果显示，FGF14基因不同基因型对16月龄延边黄牛背高和尻长均具有显著影响（P<0.05），对24月龄延边黄牛体高、体重、十字部高均具有显著影响（P<0.05）。综上所述，推测FGF14基因对延边黄牛生长发育有一定影响，为后续延边黄牛肉质性状的现代分子育种选育工作提供参考。

关键词: 延边黄牛; FGF14基因; SNP; 生长性状

Abstract: The purpose of this study was to explore the polymorphism of exon 3 of fibroblast growth factor 14 (FGF14) gene and its effect on growth traits in Yanbian Yellow cattle.The third exon of FGF14 gene was sequenced and analyzed by DNA direct sequencing method,and the genotyping of FGF14 gene was detected by HRM typing technique with high resolution melting curve,so as to explore the relationship between different genotypes of FGF14 gene and growth traits of 16 and 24 months of age in Yanbian Yellow cattle.The results showed that one A>G mutation was detected at position 631 947 bp of FGF14 gene,two alleles of A and G,and three genotypes of AA,AG,and GG.The results of χ² test showed that the distribution of genotype frequency and allele frequency of FGF14 gene in two Yanbian Yellow cattle populations of 16 and 24 months of age conformed to Hardy-Weinberg equilibrium (P>0.05).Polymorphic information content (PIC) analysis results showed that FGF14 gene was moderately polymorphic in both groups (0.25 < PIC < 0.5).The results of association analysis with growth traits showed that the different genotypes of FGF14 gene had significant influence on the traits of height at back and rump length of Yanbian Yellow cattle at 16 months of age (P<0.05),and had significant influence on the traits of body height,body weight and height at hip cross of Yanbian Yellow cattle at 24 months of age (P<0.05).In conclusion,it was speculated that FGF14 gene had a certain influence on the growth and development of Yanbian Yellow cattle,and provided reference for the subsequent modern molecular breeding and breeding of meat traits in Yanbian Yellow cattle.

Key words: Yanbian Yellow cattle; FGF14 gene; SNP; growth traits

中图分类号:

S813.3

王思寒, 田佺, 柳海星, 金花子, 李钟淑, 戢爽. 延边黄牛FGF14基因多态性及其对生长性状的影响[J]. 中国畜牧兽医, 2020, 47(12): 3993-3999.

WANG Sihan, TIAN Quan, LIU Haixing, JIN Huazi, LI Zhongshu, JI Shuang. Polymorphism of FGF14 Gene and Its Effect on Growth Traits in Yanbian Yellow Cattle[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(12): 3993-3999.

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