鸽源阿哥纳沙门菌的分离鉴定和生物学特性研究

doi:10.16431/j.cnki.1671-7236.2020.06.035

摘要/Abstract

摘要： 为了解云南某鸽场的肉鸽发病原因，拟定治疗方案，试验无菌采集病鸽肝脏组织，划线于麦康凯培养基进行细菌分离培养、生化鉴定、血清型鉴定、16S rDNA同源性分析、动物回归试验、药敏试验及毒力分析。结果显示，在麦康凯培养基上可见圆形、光滑、无色半透明的菌落，初步鉴定为革兰氏阴性菌；经沙门菌属血清凝集试验和16S rDNA同源性分析，鉴定该菌为阿哥纳沙门菌（Salmonella Agona），其抗原结构式为：O抗原1（+）、4（+）、12（+），H抗原fg（+）、s（+），将其命名为SSYN036株。细菌生长曲线测定表明，该菌株生长速度较快；动物试验表明，该菌株对试验小鼠致病强，接种浓度为5×10⁸、5×10⁷、5×10⁶和5×10⁵ CFU/mL的小鼠在7 d内死亡率分别为100%、100%、60%和30%；病理切片显示产生明显的多灶性坏死炎症及炎性细胞浸润；药敏试验表明，该菌株对青霉素、苯唑西林、链霉素、强力霉素和四环素存在耐药，对利福平中度敏感，对阿莫西林、氨苄西林、庆大霉素、妥布霉素和头孢他啶等11种抗菌药物敏感；菌株毒力基因检测表明，菌株含有spvB、spiA、pagC、msgA、invA、sipB、prgH、spaN、tolC、iroN、sitC、lpfC、sifA、sopB和pefA共15种毒力基因。本研究为了解鸽源沙门菌的致病性和菌株生物学特性提供了新的信息，具有重要的公共卫生学意义。

关键词: 鸽; 阿哥纳沙门菌; 分离鉴定; 生物学特性; 药敏试验

Abstract: In order to understand the cause of the disease in a pigeon farm in Yunnan,a treatment plan was developed.The liver tissues of sick pigeons were collected aseptically and streaked on MacConkey medium for bacterial isolation and culture,biochemical identification,serotype identification,16S rDNA homology analysis,animal regression experiments,drug sensitivity tests and virulence analysis.The results showed that round,smooth,colorless and translucent colonies were observed on the MacConkey medium,which were initially identified as Gram-negative bacteria.The strain was identified as Salmonella Agona by Salmonella serum agglutination test and 16S rDNA homology analysis.Its antigenic structural formula was O antigen 1(+),4(+),12(+),H antigen fg(+),s(+),which was named SSYN036 strain.The growth curve of bacteria showed that the strain grow faster.Animal experiments showed the strain was highly pathogenic to the test mice,the inoculated with 5×10⁸,5×10⁷,5×10⁶ and 5×10⁵ CFU/mL of mice,within 7 days,which mortality were 100%,100%,60% and 30%,respectively.The pathological sections showed more obvious focal necrotic inflammation and inflammatory cell infiltration.The drug sensitivity test showed that the strain was resistant to penicillin,oxacillin,streptomycin,doxycycline and tetracycline,moderately sensitive to rifampicin,and sensitive to amoxicillin,ampicillin,gentamicin,tobramycin,ceftazidime,etc.11 antibacterial drugs.Virulence gene test showed that the strain contains spvB,spiA,pagC,msgA,invA,sipB,prgH,spaN,tolC,iroN,sitC,lpfC,sifA,sopB and pefA genes.This study provided new data information of the pathogenicity of Salmonella and the biological characteristics of strains,and its public health significance was worthy of attention.

Key words: pigeon; Salmonella Agona; isolation and identification; biological characteristics; drug susceptibility test

中图分类号:

S852.61

李廷翠, 李珂, 严红亚, 赵蓉, 汤雅淇, 常志顺, 邹丰才, 信爱国. 鸽源阿哥纳沙门菌的分离鉴定和生物学特性研究[J]. 中国畜牧兽医, 2020, 47(6): 1952-1960.

LI Tingcui, LI Ke, YAN Hongya, ZHAO Rong, TANG Yaqi, CHANG Zhishun, ZOU Fengcai, XIN Aiguo. Isolation,Identification and Biological Characteristics of a Pathogon of Salmonella Agona Originated from Pigeon[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1952-1960.

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