›› 2009, Vol. 36 ›› Issue (6): 82-84.

• 遗传繁育 • 上一篇    下一篇

不同猪种ESR基因多态性的PCR-RFLP分析

曹晶晶1,蔡家嘉1,黄小国2,何晓亮2,包文斌1,吴圣龙1   

  1. (1.扬州大学动物科学与技术学院, 扬州 225009; 2.常州市康乐农牧有限公司, 常州 213149)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-06-20 发布日期:2009-06-20
  • 通讯作者: 吴圣龙

Polymorphisms of Porcine ESR Gene in Different Breeds with PCR-RFLP Method

CAO Jing-jing1,CAI Jia-jia1, HUANG Xiao-guo2,HE Xiao-liang2,BAO Wen-bin1,WU Sheng-long1   

  1. (1.Animal Science and Technology College, Yangzhou University, Yangzhou 225009, China;2.Kang Le Farming and Stockbreeding Co. Ltd., Changzhou City, Changzhou 213149, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-06-20 Published:2009-06-20
  • Contact: WU Sheng-long

摘要: 运用PCR-RFLP方法,检测了杜洛克、约克夏、长白猪、皮特兰、梅山猪、二花脸猪、枫泾猪、荣昌猪、苏太猪等9个国内外猪种的ESR基因PvuⅡ位点多态性。结果表明,在约克夏猪、二花脸猪、枫泾猪和苏太猪中检测到了AA、AB、BB 3种基因型,在杜洛克猪、皮特兰猪中检测到AB、BB 2种基因型,在长白猪、梅山猪、荣昌猪中只检测到了AB基因型。在本研究中不同品种的A、B基因频率差异显著,总体上在国外猪群体中A等位基因为优势等位基因,而地方猪种中B等位基因为优势等位基因。

关键词: 猪; PCR-RFLP; ESR基因; 多态性

Abstract: PvuⅡ polymorphisms of ESR gene were analyzed in nine native and exotic pig breeds including Duroc, Yorkshire, Landrace, Pietrain, Meishan, Erhualian, Fengjing, Rongchang and Sutai pigs with PCR-RFLP method. The results showed that AA, AB, BB genotypes were detected in Yorkshire pigs, Erhualian pigs, Fengjing pigs and Sutai pigs, while only AB and BB genotypes were found in Duroc pigs and Pietrain pigs, only AB genotypes were detected in Landrace pigs, Meishan pigs and Rongchang pigs. There was a significant difference in gene frequencies and genotype distribution among different breeds. The allele A was dominant allele in domestic pig breeds while the allele B was dominant allele in native pig breeds.

Key words: pig; ESR gene; PCR-RFLP; polymorphism

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