RT-PCR技术检测绵羊卵巢内Ghrelin的表达

›› 2009, Vol. 36 ›› Issue (6): 79-81.

RT-PCR技术检测绵羊卵巢内Ghrelin的表达

斯琴高娃，杜晨光

(内蒙古农业大学职业技术学院，包头 014109)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-06-20 发布日期:2009-06-20
通讯作者: 杜晨光

RT-PCR Detecting Expression of Ghrelin in the Antral Follicles of Sheep Ovary

Siqingaowa, DU Chen-guang

(Vocational and Technical College, Inner Mongolia Agricultural University, Baotou 014109， China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-06-20 Published:2009-06-20
Contact: DU Chen-guang

摘要/Abstract

摘要： 为了明确Ghrelin在绵羊卵巢有腔卵泡上是否存在表达，本研究利用实时定量RT-PCR技术检测了绵羊卵巢有腔卵泡内的卵母细胞、卵丘细胞和壁层颗粒细胞的Ghrelin蛋白的表达量情况。结果揭示绵羊卵巢有腔卵泡内各类型细胞Ghrelin mRNA的相对表达量大致相同。绵羊卵巢有腔卵泡内各类型细胞Ghrelin蛋白的表达及Ghrelin mRNA的表达模式，尤其是卵母细胞中的表达，揭示这一新型分子在绵羊卵巢上具有潜在的调控作用。

关键词: Ghrelin; 绵羊有腔卵泡; 实时定量RT-PCR

Abstract: The possibility of Ghrelin expressed in antral follicles of sheep ovary was investigated in this study， Ghrelin protein was detected in cumulus cells (CCs) and granulosa cells (GCs). Relative real-time reverse-transciptase (RT)-PCR experiments confirmed that the Ghrelin mRNA levels did not show significantly difference. The persistent presence of the Ghrelin signaling system within antral follicles, including oocytes, CCs and GCs, revealed the possibility of a potential regulatory role of this novel molecule during oocyte maturation and embryonic development.

Key words: Ghrelin; sheep antral follicles; real time RT-PCR

中图分类号:

Q786

斯琴高娃;杜晨光. RT-PCR技术检测绵羊卵巢内Ghrelin的表达[J]. , 2009, 36(6): 79-81.

Siqingaowa;DU Chen-guang. RT-PCR Detecting Expression of Ghrelin in the Antral Follicles of Sheep Ovary[J]. China Animal Husbandry & Veterinary Medicine, 2009, 36(6): 79-81.

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