关键词: 猫细小病毒; 免疫球蛋白; 制备

Abstract: Feline panleukopenia is an infectious disease that infected more and more animals by FPV upward momentum. It has high pathogenicity and mortality. Treatment of the disease had not better and effective drug at home and abroad. The test selected horses as immune animals and extracted its serum. We optimized a new method of purification of IgG, by digesting the serum at 37 ℃ with pepsase, removing albumin in caprylic acid, and the twice ammonium sulfate precipitation. According to the character that Protein A and Fc of immunoglobulin can connect with IgG of most mammals, the F(ab′)2 was collected and purified by Protein A column filter.According to the relavant items of “People’s Republic of China States Pharmacopoeia”(2005 version). The physical properties, heat antigenicity, safety, aseptic, HA and HI test, agarose double diffusion, cells neutralization test and animal treatment test of this biological product of horse anti-FPV IgG were examined. The results showed that the product is a milk white solution that is non-layered, free of visible particles, bacteria, fungi, mycoplasma and other microbial contaminations. Rabbits, mice, and guinea pigs injected with the product were observed for 10 successive days. The results showed that the product is highly specific with the titer of 128-fold, good security and stability, non-toxic side effects. F(ab′)2 molecular weight of about 110 ku, agents accounted for 70.7% of total protein. The clinical symptoms of all the 5 cats disappeared 7 days later with the recovery rate of 100%, indicating the high effectiveness of the product in treatment of feline panleukopenia.

Key words: feline panleukopenia virus; IgG; preparation