乳酸菌制剂对肉仔鸡鼠伤寒沙门菌感染的控制效果研究

doi:10.16431/j.cnki.1671-7236.2020.03.025

摘要/Abstract

摘要： 本试验旨在研究乳酸菌制剂对肉仔鸡鼠伤寒沙门菌（ST）感染的控制效果。选择无ST感染的1日龄AA公雏480只，随机分为4组，每组6个重复，每个重复20只。其中未感染组饲喂基础日粮；感染组饲喂基础日粮且沙门菌感染；抗生素组在基础日粮中添加40 mg/kg盐酸恩诺沙星；乳酸菌组在基础日粮中添加3×10⁹ CFU/kg乳酸菌制剂（植物乳杆菌、噬酸乳杆菌和屎肠球菌等比例混合）。5日龄时，除未感染组外，其他各组每只鸡灌服1 mL（10⁵ CFU）ST液。7、14和21日龄时，每个重复随机选2只鸡，采血、屠宰、取样。结果显示，ST感染显著降低了1~21日龄肉仔鸡的平均日采食量和平均日增重，日粮中添加乳酸菌制剂显著提高了平均日采食量和平均日增重（P<0.05），且达到了未感染组和抗生素组水平。基于器官指数和组织载菌量，乳酸菌制剂对肉仔鸡ST感染表现出显著的控制效果（P<0.05）。14、21日龄时，乳酸菌组和抗生素组白细胞介素-10（IL-10）含量均显著高于未感染组（P<0.05）；21日龄时，乳酸菌组肉仔鸡血清IL-10含量显著低于感染组，免疫球蛋白G（IgG）和分泌型免疫球蛋白A（SIgA）含量显著升高（P<0.05）；乳酸菌组肉仔鸡血清IL-10和SIgA含量与抗生素组间差异不显著（P>0.05），而IgG水平显著低于抗生素组（P<0.05）。综上，日粮中添加乳酸菌制剂能显著抑制肉仔鸡肠道ST的数量及组织感染，能显著缓解炎症反应、提高免疫水平和生产性能。

关键词: 肉仔鸡; 白细胞介素-10(IL-10); 免疫球蛋白; 乳酸菌制剂; 鼠伤寒沙门菌

Abstract: This study was aimed to investigate the effects of lactic acid bacteria (LAB) preparationon on Salmonella Typhimurium (ST) infection of broiler chickens.A total of 480 one-day old AA male broiler chickens without ST infection were randomly allotted into 4 groups with 6 replicates per group and 20 birds per replicate.The treatments included uninfection (a basal diet),ST infection,and based on the infection,supplemented with antibiotic enrofloxacin HCl or LAB at 40 mg/kg or 3×10⁹ CFU/kg,respectively.The LAB contained Lactobacillus plantarum,Lactobacillus acidophilus and Enterococcusfaecium combined at an equal amount.At 5 days old in infected groups the chickens were orally inoculated with 1 mL (10⁵ CFU) ST bacterial fluid.At 7,14 and 21 days old,2 birds per replicate were randomly selected and killed for sampling.The results showed that ST infection decreased average daily feed intake(ADFI) and average daily gain(ADG) of broilers during 1-21 days old,while LAB supplementation compensated the growth performance (P<0.05),and reached the levels of antibiotic and uninfected groups.In terms of organ index and bacterial loads in tissues,LAB exhibited a good control effect on broilers with ST infection (P<0.05).At 14 and 21 day olds,the content of IL-10 in LAB and antibiotic groups were significantly higher than that of uninfected group (P<0.05).At 21 days old,the content of IL-10 in LAB group was significantly lower than that of infected group (P<0.05),the contents of IgG and SIgA were significantly increased (P<0.05).There was no significant difference of the contents of IL-10 and SIgA between LAB and antibiotic groups (P>0.05),but the content of IgG in LAB group was significantly lower than that of antibiotic group (P<0.05).In conclusion,dietary LAB supplementation could depress gut ST proliferation and tissue infection,alleviated inflammation,and improved immunity and growth performance in broilers.

Key words: broiler; IL-10; immunoglobulin; lactic acid bacteria; Salmonella Typhimurium

中图分类号:

Q939.11⁺7

郑江平, 刘宁, 尚月丽, 白雪瑞. 乳酸菌制剂对肉仔鸡鼠伤寒沙门菌感染的控制效果研究[J]. 中国畜牧兽医, 2020, 47(3): 856-863.

ZHENG Jiangping, LIU Ning, SHANG Yueli, BAI Xuerui. Effects of Lactic Acid Bacteria Preparation on Salmonella Typhimurium Infection of Broiler Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(3): 856-863.

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