栀子四黄散抗猪繁殖与呼吸综合征的研究

doi:10.16431/j.cnki.1671-7236.2020.11.026

摘要/Abstract

摘要： 为探索栀子四黄散对猪繁殖与呼吸综合征（porcine reproductive and respiratory syndrome，PRRS）的治疗效果，本研究将45头组随机分为对照组、病毒组及中药高、中、低剂量组5组，每味中药400目超微粉碎后分别添加饲喂接种猪繁殖与呼吸综合征病毒（porcine reproductive and respiratory syndrome virus，PRRSV）的试验猪，连续饲喂14 d，每日观察临床症状，7、14和21 d时随机采集5组血清样品进行细胞因子检测，并进行病理剖检和采集肺脏样品进行病理组织切片观察。结果显示，添加中药7 d后临床症状均减轻，中剂量和高剂量组第14天时体温恢复正常，呼吸困难症状消失，食欲恢复，中药治疗组肺脏未见肉样变，肺脏表面有出血点或出血斑。中剂量组肺泡间隔增厚，轻度淤血，肺实质轻度炎性细胞浸润。病毒组猪血清中IgG和γ-干扰素（IFN-γ）含量逐渐降低，白介素-10（IL-10）含量逐渐上升。添加中药的3组血清中IgG含量均高于病毒组，并且IgG含量均随时间延长逐渐上升，中剂量组血清中IgG含量均极显著高于对照组和病毒组（P<0.01）；添加中药的3组IFN-γ含量逐渐上升，14和21 d时中剂量组血清中IFN-γ含量均极显著高于对照组和病毒组（P<0.01）；中剂量组血清中IL-10含量逐渐降低，14和21 d时与病毒组差异极显著（P<0.01），与对照组差异不显著（P>0.05）。综上，栀子四黄散可有效改善试验猪临床症状、病理变化和细胞因子的分泌，中剂量组效果最好。本研究为中药防治PRRS提供了试验依据。

关键词: 猪繁殖与呼吸综合征病毒(PRSSV); 免疫球蛋白G; γ-干扰素; 白介素-10; 栀子四黄散

Abstract: In order to investigate the therapeutic effect of Gardenia Sihuang powder on porcine reproductive and respiratory syndrome,45 pigs were randomly divided into five groups:Control group,virus group,low dose Chinese medicine prescription group,the middle dose Chinese medicine prescription group and the high dose Chinese medicine prescription group.Experimental pigs with porcine reproductive and respiratory syndrome virus (PRRSV) were added to each herb after 400 MTS of ultricro grinding,After continuous feeding for 14 days,clinical symptoms were observed daily.Five groups of serum samples were randomly collected for cytokine detection at 7,14 and 21 d,and pathological autopsy and lung samples were collected for pathological tissue section observation.The results showed that after adding traditional Chinese medicine for 7 days,the clinical symptoms were all relieved.At 14 days,the body temperature returned to normal,the symptoms of dyspnea disappeared,and the appetite recovered in middle and high dose groups.No meat-like changes were observed in the lungs of the traditional Chinese medicine treatment group,and there were bleeding spots or blood spots on the surface of the lungs.In the middle dose group,alveolar septa were thickened,blood congestion was mild,and lung parenchyma was mildly inflammatory cell infiltration.The levels of IgG and IFN-γ in the serum of the virus group decreased gradually,while the levels of IL-10 increased gradually.The contents of IgG in the serum of the three groups added with traditional Chinese medicine were higher than that of the virus group,and the content of IgG gradually increased.The content of IgG in the serum of the middle dose group was extremely significantly higher than that of the control group and the virus group (P<0.01).The content of IFN-γ in the three groups with traditional Chinese medicine gradually increased,and the serum content of IFN-γ in the middle dose group was extremely significantly higher than that of the control group and the virus group (P<0.01).The serum IL-10 content in the middle dose group gradually decreased,and the difference with the virus group was extremely significant (P<0.01),but not significant with the control group (P>0.05) at 14 and 21 days.The results of this study showed that fructus Gardenia Sihuang powder could effectively improve clinical symptoms,pathological changes and cytokine secretion in experimental pigs.It provided experimental basis for the prevention and treatment of porcine reproductive and respiratory syndrome.

Key words: porcine reproductive and respiratory syndrome virus (PRSSV); IgG; IFN-γ; IL-10; fructus Gardenia Sihuang powder

中图分类号:

S854.5⁺1

解慧梅, 蔡丙严, 郝福星, 郭广富, 蒋文清, 刘佳宁, 程汉. 栀子四黄散抗猪繁殖与呼吸综合征的研究[J]. 中国畜牧兽医, 2020, 47(11): 3633-3640.

XIE Huimei, CAI Bingyan, HAO Fuxing, GUO Guangfu, JIANG Wenqing, LIU Jianing, CHENG Han. Study on Resistance of Fructus Gardeniae Sihuang Powder to Porcine Reproductive and Respiratory Syndrome[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(11): 3633-3640.

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