绒山羊次级毛囊基因表达滞后性的转录组分析

doi:10.16431/j.cnki.1671-7236.2016.07.006

《中国畜牧兽医》---唯一指定的官方网站 ›› 2016, Vol. 43 ›› Issue (7): 1694-1701.doi: 10.16431/j.cnki.1671-7236.2016.07.006

绒山羊次级毛囊基因表达滞后性的转录组分析

季小阳^1,2,3, 刘斌^1,2,3, 白雪^1,2,3, 王宏^1,2,3, 付绍印², 苍真伟⁴, 吴江鸿², 张文广¹

1. 内蒙古农业大学动物科学学院, 动物遗传育种与繁殖自治区重点实验室, 呼和浩特 010018;
2. 内蒙古ATCG生物信息研究所, 呼和浩特 010020;
3. 内蒙古犇牛科技有限公司, 呼和浩特 010018;
4. 大连市畜牧总站, 大连 116037

收稿日期:2016-01-27 出版日期:2016-07-20 发布日期:2016-07-22
通讯作者: 张文广 E-mail:actgnm.bi@aliyun.com
作者简介:季小阳(1990-),女,辽宁大连人,硕士生,研究方向:绒山羊遗传育种与繁殖,E-mail:jxyang0716@126.com;刘斌(1988-),男,山西晋中人,硕士生,研究方向:绒山羊遗传育种与繁殖,E-mail:liu207702@163.com
基金资助:
国家自然科学基金（31260538、30960246）；内蒙古重大科技支撑项目

Transcriptome Survey Reveals Hysteresis in Secondary Hair Follicle for Gene Regulation in Cashmere Goat

JI Xiao-yang^1,2,3, LIU Bin^1,2,3, BAI Xue^1,2,3, WANG Hong^1,2,3, FU Shao-yin², CANG Zhen-wei⁴, WU Jiang-hong², ZHANG Wen-guang¹

1. Key Laboratory of Animal Genetics, Breeding and Reproduction of the Inner Mongolia Autonomous Region, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Institute of ATCG, Nei Mongol Bio-information, Hohhot 010018, China;
3. Nei Mongol BioNew Technology Co., Ltd., Hohhot 010018, China;
4. Dalian Animal Husbandry Station, Dalian 116037, China

Received:2016-01-27 Online:2016-07-20 Published:2016-07-22

摘要/Abstract

摘要：

毛囊是具有高度的自我更新能力、独特的可再生器官，其周期性变化是一个动态过程，为了研究毛囊差异基因表达变化情况，文章以内蒙古绒山羊生长期毛囊为研究对象，利用Illumina高通量测序技术方法，检测6~10月皮肤毛囊差异基因表达变化情况，寻找对绒毛生长及质量具有调控作用的特异表达基因。试验发现，多数差异基因分别在初级毛囊（PF）的7、8月份和次级毛囊的（SF）8、9月份表达；在绒毛生长期的相邻月份中，发现FAM83C、LOC102185501、FYCO1、CTTNBP2NL、C6orf132、KRTAP29-1 和 CRYBG3 7个共同差异表达基因呈现相同的表达趋势，且在次级毛囊上的表达变化均比初级毛囊滞后1个月。综上所述，次级毛囊在基因表达调控中存在一定的滞后性，这将为绒毛周期生长发育和分子育种提供重要的理论基础。

关键词: 绒山羊; 毛囊; 滞后性; 转录组

Abstract:

Hair follicle is capable of self-renew and its growth cycle is a dynamic process.In order to investigate the vary of differential expression genes in the Nei Mongol cashmere goat hair follicles during anagen,the mRNA expression levels of different genes in skin hair follicles were detected from June to October using Illumina technology.Evaluation of transcriptomes was undertaken to understand the time-course gene expression involved in both follicle types.The samples were collected from June to October.Most of differentially expressed genes appeared between July to August in primary hair follicle (PF) and between August to September in secondary hair follicle (SF).Seven differentially expressed genes,FAM83C,LOC102185501,FYCO1,CTTNBP2NL,C6orf132,KRTAP29-1 and CRYBG3 were identified.Their expression demonstrated similar trends at different months of cashmere growth cycle and the SF postpone for about one month.These findings indicated the hysteresis in SF gene regulation comparing with PF,and could provide the useful theoretical basis for the molecular mechanism of cashmere regeneration.

Key words: cashmere goat; hair follicle; hysteresis; transcriptome

中图分类号:

Q344⁺.13

季小阳, 刘斌, 白雪, 王宏, 付绍印, 苍真伟, 吴江鸿, 张文广. 绒山羊次级毛囊基因表达滞后性的转录组分析[J]. 《中国畜牧兽医》---唯一指定的官方网站, 2016, 43(7): 1694-1701.

JI Xiao-yang, LIU Bin, BAI Xue, WANG Hong, FU Shao-yin, CANG Zhen-wei, WU Jiang-hong, ZHANG Wen-guang. Transcriptome Survey Reveals Hysteresis in Secondary Hair Follicle for Gene Regulation in Cashmere Goat[J]. , 2016, 43(7): 1694-1701.

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绒山羊次级毛囊基因表达滞后性的转录组分析

Transcriptome Survey Reveals Hysteresis in Secondary Hair Follicle for Gene Regulation in Cashmere Goat

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