不同组学视角分析中药抗病毒作用研究进展

doi:10.16431/j.cnki.1671-7236.2025.02.006

摘要/Abstract

摘要： 病毒是引起疾病的重要微生物之一，严重危害了人类和动物的健康及生命安全。随着病毒的不断变异与重组，如流感病毒、新型冠状病毒、猪繁殖与呼吸综合征病毒等，使得疫苗提供的保护有限。同时，有效抗病毒药物的缺乏使得病毒感染后治疗困难，因此，迫切需要寻找新的、安全、有效的防控方法来预防病毒感染。中药因具有成分安全、不易产生耐药性且能多途径、多靶点作用于病毒等优点而被广泛应用于抗病毒研究中。传统中药为抗病毒药物提供了丰富的基础，但传统试验手段进行抗病毒药物研发耗时久且工作量大，组学技术的发展为抗病毒药物研发提供了新的视角，利用组学数据分析药物和疾病之间的关系已成为筛选、评价药物疗效和发现新的药物靶点的有力手段。因此，笔者总结分析了转录组学、蛋白质组学、代谢组学及多组学联合分析在中药抗病毒中的研究进展，通过不同组学视角分析中药在抗病毒研究中的应用，不仅有助于深入理解中药的抗病毒机制，还可为抗病毒药物的研发和优化提供科学依据。

关键词: 中药; 抗病毒作用; 转录组学; 蛋白质组学; 代谢组学; 多组学联合分析

Abstract: Viruses are one of the most important microorganisms causing diseases,which seriously endanger the health and life safety of human beings and animals.With the constant variation and recombination of viruses,such as Influenza virus,Severe acute respiratory syndrome coronavirus 2 and Porcine reproductive and respiratory syndrome virus,the protection provided by vaccines is limited.At the same time,the lack of effective antiviral drugs makes it difficult to treat viral infections.Therefore,it is an urgent need to find a new,safe and effective prevention and control methods to prevent viral infections.Traditional Chinese medicines have been widely used in antiviral research because of their safe composition,low resistance to drugs,and their ability to act on viruses in multiple ways and at multiple targets.Traditional Chinese medicine provides a rich base for antiviral drugs,but traditional experimental methods for antiviral drug development is time-consuming and heavy workload,the development of omics technology provides a new perspective for antiviral drug development,the use of omics data to analyze the relationship between drugs and diseases has become a powerful means of screening,evaluating the efficacy of drugs and discovering new drug targets.Therefore,the authors summarize and analyze the progress of transcriptomics,proteomics,metabolomics and multi-omics integration analysis in the antiviral research of traditional Chinese medicine,and analyze the application of traditional Chinese medicine in antiviral research through different omics perspectives,which not only contributes to an in-depth understanding of the antiviral mechanism of traditional Chinese medicine,but also provides a scientific basis for the research and development and optimization of antiviral drugs.

Key words: traditional Chinese medicine; antiviral effect; transcriptomics; proteomics; metabolomics; multi-omics integration analysis

中图分类号:

S853.74

罗琴, 刘丁语, 刘宝玲, 乔常宏, 陈翔宇, 何振文, 王晓虎, 陈晶, 张翩, 黄元, 王刚, 刘昊, 蔡汝健. 不同组学视角分析中药抗病毒作用研究进展[J]. 中国畜牧兽医, 2025, 52(2): 554-561.

LUO Qin, LIU Dingyu, LIU Baoling, QIAO Changhong, CHEN Xiangyu, HE Zhenwen, WANG Xiaohu, CHEN Jing, ZHANG Pian, HUANG Yuan, WANG Gang, LIU Hao, CAI Rujian. Research Progress on Antiviral Effect of Traditional Chinese Medicine Analyzed from Different Omics Perspectives[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(2): 554-561.

参考文献

[1] ZANDI K,MUSALL K,OO A,et al.Baicalein and baicalin inhibit SARS-CoV-2 RNA-dependent-RNA polymerase[J].Microorganisms,2021,9(5):893.
[2] LIU X,ZHU Y,WANG D,et al.The natural compound Sanggenon C inhibits PRRSV infection by regulating the TRAF2/NF-kappaB signalling pathway[J].Veterinary Research,2023,54(1):114.
[3] HUAN C,XU Y,ZHANG W,et al. Hippophae rhamnoides polysaccharides dampen Pseudorabies virus infection through downregulating adsorption,entry and oxidative stress[J].International Journal of Biological Macromolecules，2022,207:454-463.
[4] HUAN C,XU Y,ZHANG W,et al.Glycyrrhiza polysaccharide inhibits Pseudorabies virus infection by interfering with virus attachment and internalization[J]. Viruses,2022;14(8):1772.
[5] FANG S,DONG L,LIU L,et al.HERB:A high-throughput experiment- and reference-guided database of traditional Chinese medicine[J].Nucleic Acids Research,2021,49(D1):D1197-D1206.
[6] PAN Y,LEI X,ZHANG Y.Association predictions of genomics,proteinomics,transcriptomics,microbiome,metabolomics,pathomics,radiomics,drug,symptoms,environment factor,and disease networks:A comprehensive approach[J].Medicinal Research Reviews,2022,42(1):441-461.
[7] 崔凯,吴伟伟,刁其玉.转录组测序技术的研究和应用进展[J].生物技术通报,2019,35(7):1-9.CUI K,WU W W,DIAO Q Y.Application and research progress on transcriptomics[J].Biotechnology Bulletin,2019,35(7):1-9.(in Chinese)
[8] 陈玲.紫杉醇对乙型肝炎病毒转录复制的影响及其机制研究[D].重庆：重庆医科大学,2022.CHEN L.The effect and mechanism of paclitaxel on the transcription and replication of Hepatitis B virus[D].Chongqing:Chongqing Medical University,2022.(in Chinese)
[9] 沈毓峰.牛蒡子苷元衍生物抗鲤春病毒血症病毒活性及机制研究[D].杨凌：西北农林科技大学,2021.SHEN Y F.Antiviral activity and mechanism of arctigenin derivatives against Spring viraemia of carp virus[D].Yangling：Northwest A&F University,2021.(in Chinese)
[10] 陈小龙.番石榴叶抗拟穴青蟹病毒病机制初探及抗菌中草药制剂的研制[D].上海：上海海洋大学,2020.CHEN X L.Study on the mechanism of guava leaf against viral disease of Scylla paramamosain and development of antibacterial Chinese herbal compound preparation[D].Shanghai:Shanghai Ocean University,2020.(in Chinese)
[11] 张祎桐.EGCG及其代谢产物ECG抗草鱼呼肠孤病毒及白斑综合征病毒的研究[D].上海：上海海洋大学,2020.ZHANG Y T.Study on the resistance of EGCG and its metabolite ECG to GCRV and WSSV[D].Shanghai:Shanghai Ocean University,2020.(in Chinese)
[12] 杨稳.穿心莲内酯抑制流感病毒所致肺炎的作用机制研究[D].济南：山东中医药大学,2023.YANG W.The underlying mechanism of andrographolide for suppressing Influenza virus-induced pneumonia[D].Jinan:Shandong University of Traditional Chinese Medicine,2023.(in Chinese)
[13] 司马明威.茶黄素-3'-没食子酸酯(TF2b)抗甲型H1N1流感病毒活性及其作用机制研究[D].长春：长春中医药大学,2023.SIMA M W.Study on the activity and mechanism of theaflavin-3'-galate (TF2b) against H1N1 Influenza A virus[D].Changchun:Changchun University of Chinese Medicine,2023.(in Chinese)
[14] 沈飞.EGCG对嗜水气单胞菌和鲤疱疹病毒Ⅱ型的抑制作用研究[D].上海：上海海洋大学,2022.SHEN F.Inhibitory effect of EGCG on Aeromonas hydrophila and Cyprinid herpesvirus 2[D].Shanghai:Shanghai Ocean University,2022.(in Chinese)
[15] WISNIEWSKI J R,MANN M.A proteomics approach to the protein normalization problem:Selection of unvarying proteins for MS-based proteomics and Western blotting[J].Journal of Proteome Research,2016,15(7):2321-2326.
[16] CAKIR M,OBERNIER K,FORGET A,et al.Target discovery for host-directed antiviral therapies:Application of proteomics approaches[J]. mSystems,2021,6(5):e38821.
[17] 谭子涵,王帅杰,胡玉妍,等.槲皮素干预猪流行性腹泻病毒感染幼龄仔猪回肠蛋白质组的研究[J].饲料研究,2022,45(15):36-41.TAN Z H,WANG S J,HU Y Y,et al.Effect of quercetin on ileal proteome of young piglets infected with Porcine epidemic diarrhea virus[J].Feed Research,2022,45(15):36-41.(in Chinese)
[18] WU M,ZHANG Q,YI D,et al.Quantitative proteomic analysis reveals antiviral and anti-inflammatory effects of puerarin in piglets infected with Porcine epidemic diarrhea virus[J]. Frontiers in Immunology,2020,11:169.
[19] 徐婷玉,匡琳,石佳灵,等.基于网络药理学和蛋白质组学探讨知柏地黄汤抗HSV-2的作用机制[J].中国皮肤性病学杂志,2023,36(7):1-16.XU T Y,KUANG L,SHI J L,et al.Exploring the mechanism of Zhibai Dihuang Tang’s anti HSV-2 effect based on network pharmacology and proteomics[J].The Chinese Journal of Dermatovenereology,2023,36(7):1-16.(in Chinese)
[20] WEI J,SUN J,ZENG J,et al.Precise investigation of the efficacy of multicomponent drugs against pneumonia infected with Influenza virus[J].Frontiers in Pharmacology,2021,12:604009.
[21] 张司寰.血根碱抗PCV2感染的作用及机制研究[D].太谷：山西农业大学,2023.ZHANG S H.Effect and mechanism of sanguinarine against PCV2 infection[D].Taigu:Shanxi Agricultural University,2023.(in Chinese)
[22] YANG L,ZHANG J H,ZHANG X L,et al.Tandem mass tag-based quantitative proteomic analysis of lycorine treatment in highly pathogenic Avian influenza H5N1 virus infection[J].PeerJ,2019,7:e7697.
[23] ZHANG H,CAO Z,SUN P,et al.A novel strategy for optimal component formula of anti-PRRSV from natural compounds using tandem mass tag labeled proteomic analyses[J].BMC Veterinary Research,2022,18(1):179.
[24] 黄小丹,陈梦雨,黄文洁,等.基于代谢组学的植物多酚及其肠道健康效应研究进展[J].生物技术通报,2021,37(1):123-136.HUANG X D,CHEN M Y,HUANG W J,et al.Progress based on metabolomics:Plant polyphenols and their gut health benefit[J].Biotechnology Bulletin,2021,37(1):123-136.(in Chinese)
[25] 包芳,李羽涵,杨志刚.甘草代谢组学的研究进展[J].中草药,2018,49(19):4662-4669.BAO F,LI Y H,YANG Z G.Advances in metabolomics research of Glycyrrhizae Radix et Rhizoma[J]. Chinese Traditional and Herbal Drugs,2018,49(19):4662-4669.(in Chinese)
[26] 李娜,杜海涛,王晓雪,等.黄芩苷抗呼吸道合胞病毒的细胞代谢组学研究[J].中国药理学通报,2023,39(4):750-757.LI N,DU H T,WANG X X,et al.Study on cytometabonomics of baicalin against Respiratory syncytial virus[J].Chinese Pharmacological Bulletin,2023,39(4):750-757.(in Chinese)
[27] 谢彤,杜丽娜,徐建亚,等.基于脂质代谢网络的虎杖抗呼吸道合胞病毒肺炎代谢组学研究[J].世界中医药,2016,11(9):1670-1673.XIE T,DU L N,XU J Y,et al.Metabonomics study on the effect of Polygonum cuspidatumon anti-Respiratory syncytial virus (RSV) basing on lipid metabolism[J].World Chinese Medicine,2016,11(9):1670-1673.(in Chinese)
[28] DU L N,XIE T,XU J Y,et al.A metabolomics approach to studying the effects of Jinxin oral liquid on RSV-infected mice using UPLC/LTQ-Orbitrap mass spectrometry[J].Journal of Ethnopharmacology,2015,174:25-36.
[29] DING J,LI J,ZHANG Z,et al.Network pharmacology combined with metabolomics to explore the mechanism for Lonicerae Japonicae flos against Respiratory syncytial virus[J].BMC Complementary Medicine and Therapies,2023,23(1):449.
[30] 孙启慧.双黄连制剂抑菌抗病毒药效物质基础和组方结构配伍及作用机理研究[D].济南：山东中医药大学,2019.SUN Q H.Study on the chemical analysis and structural compatibility and mechanism of antibacterial and antiviral pharmacodynamics of Shuanghuanglian preparation[D].Jinan:Shandong University of Traditional Chinese Medicine,2019.(in Chinese)
[31] SUN Y L,ZHAO P P,ZHU C B,et al.Integrating metabolomics and network pharmacology to assess the effects of quercetin on lung inflammatory injury induced by Human respiratory syncytial virus[J].Scientific Reports,2023,13(1):8051.
[32] LI W,WANG X,CHEN Y,et al.Luteolin-7-O-glucoside promotes macrophage release of IFN-beta by maintaining mitochondrial function and corrects the disorder of glucose metabolism during RSV infection[J].European Journal of Pharmacology,2024,963:176271.
[33] 李佳铠.鬼针草提取物抑制PRRSV诱导炎症反应的作用研究[D].福州：福建农林大学,2022.LI J K.Inhibitory effect of Bidens bipinnata extract on PRRSV-induced inflammatory response[D].Fuzhou:Fujian Agriculture and Forestry University,2022.(in Chinese)
[34] 郑蕊,郭朋,刘宇,等.基于网络药理学和代谢组学的苦参乌梅汤对HepG 2.2.15细胞模型抗乙型肝炎病毒作用机制研究[J].中国医药导报,2022,19(15):12-18.ZHENG R,GUO P,LIU Y,et al.Study on the mechanism of Kushen Wumei decoction against Hepatitis B virus in HepG 2.2.15 cell model based on network pharmacology and metabolomics[J].China Medical Herald,2022,19(15):12-18.(in Chinese)
[35] FENG H,ZHANG J,ZHANG K,et al.Synergistic anti-Infectious bronchitis virus activity of phillygenin combined baicalin by modulating respiratory microbiota and improving metabolic disorders[J].Poultry Science,2024,103(2):103371.
[36] FU X,WANG Z,LI L,et al.Novel chemical ligands to Ebola virus and Marburg virus nucleoproteins identified by combining affinity mass spectrometry and metabolomics approaches[J].Scientific Reports,2016,6:29680.
[37] ZHU L, GAO N, WANG R, et al.Proteomic and metabolomic analysis of marine medaka (Oryzias melastigma) after acute ammonia exposure[J].Ecotoxicology,2018,27(3):267-277.
[38] 毕文文.基于组学研究茜草素对DEV增殖的影响[D].贵阳：贵州大学,2023.BI W W.Study on the effect of alizarin on DEV proliferation based on omics[D].Guiyang:Guizhou University，2023.(in Chinese)
[39] JIANG D,ZHANG L,ZHU G,et al.The antiviral effect of isatis root polysaccharide against NADC30-like PRRSV by transcriptome and proteome analysis[J].International Journal of Molecular Sciences,2022,23(7):3688.
[40] LIU X,LIN L,LV T,et al.Combined multi-omics and network pharmacology approach reveals the role of Tripterygium wilfordii Hook F in treating HIV immunological non-responders[J].Phytomedicine,2022,101:154103.