抗鸡传染性支气管炎病毒中药的筛选

doi:10.16431/j.cnki.1671-7236.2024.03.036

摘要/Abstract

摘要： 【目的】对鸡传染性支气管炎病毒(Infectious bronchitis virus,IBV)进行抗病毒中药的筛选,并验证其治疗效果,为临床用药提供一定参考。【方法】采用鸡胚培养法,通过预防和治疗2个角度对24味单一中药、6种市售复方中药进行抗IBV的有效成分或方剂筛选试验,即在SPF鸡胚中先注射中药2 h后再接种病毒和先接种病毒2 h后再注射中药。使用SPSS 20.0软件对鸡胚重量进行方差分析,利用实时荧光定量PCR法辅助判定治疗效果。【结果】对鸡胚净重进行方差分析显示,桔梗组与阳性对照组差异显著(P＜0.05),与阴性对照组差异不显著(P＞0.05),表明预防和治疗均有效果。实时荧光定量PCR检测鸡胚尿囊液病毒滴度结果显示,桔梗、鱼腥草、宣肺败毒方、绞股蓝、蒲公英、甘草对IBV增殖均有抑制作用,其中桔梗抑制效果最佳。【结论】桔梗对IBV有显著的预防及抑制效果,结果为深入研究桔梗作用机理提供了依据。

关键词: 鸡传染性支气管炎病毒(IBV); 鸡胚; 中药; 筛选

Abstract: 【Objective】 This study was aimed to screen the traditional Chinese medicine (TCM) against avian Infectious bronchitis virus (IBV) and verify therapeutic effect,so as to provide a reference for clinical medication.【Method】 The chicken embryo culture method was used to study the anti-IBV screening test which selected 24 single TCM and 6 commercially available compound TCM.The experimental procedure was to inject TCM in SPF chicken embryos first and then inoculate the virus 2 h later or inoculate the virus first and then inject TCM 2 h later.The variance (ANOVA) analysis of chicken embryo weight was performed with SPSS 20.0 software,and Real-time quantitative PCR was used to assist in determining the therapeutic effect.【Result】 ANOVA on the net weight of chicken embryos showed that there was significant difference between Platycodon grandiflorus (Jacq.) A.DC and positive control group (P＜0.05),and there was no significant difference with negative control group(P＞0.05),indicating that Platycodon grandiflorus (Jacq.) A.DC had preventive and curative effects.The viral load in allantoic fluid by Real-time quantitative PCR showed that Platycodon grandiflorus (Jacq.) A.DC,Houttuynia cordata Thunb,Xuanfeibaidu prescription,Gynostemma pentaphyllum (Thunb.) Makino,Taraxacum mongolicum Hand.-Mazz and Glycyrrhiza uralensis Fisch could inhibit the proliferation of IBV, and the inhibitory effect of Platycodon grandiflorus (Jacq.) A.DC was the best.【Conclusion】 Platycodon grandiflorus (Jacq.) A.DC had the remarkably effective for treating IBV,which laid a reference for the in-depth study of the mechanism of Platycodon grandiflorus (Jacq.) A.DC.

Key words: avian Infectious bronchitis virus (IBV); chicken embryo; traditional Chinese medicine; screening

中图分类号:

S852.65⁺9.6

李俊贤, 任涛, 杨剑, 郭杨, 文安林, 欧德渊. 抗鸡传染性支气管炎病毒中药的筛选[J]. 中国畜牧兽医, 2024, 51(3): 1241-1249.

LI Junxian, REN Tao, YANG Jian, GUO Yang, WEN Anlin, OU Deyuan. Screening of Traditional Chinese Medicine for Anti-avian Infectious Bronchitis Virus[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(3): 1241-1249.

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