京星黄鸡白细胞介素-2基因与H/L的关联分析

doi:10.16431/j.cnki.1671-7236.2015.08.025

摘要/Abstract

摘要： 本研究选用鸡白细胞介素2(interleukin-2,IL-2)基因作为影响鸡免疫性状的候选基因。以京星黄鸡为研究对象,采用直接测序方法对IL-2基因的5个SNPs位点的基因型频率和等位基因频率、单倍型及其对异噬性粒细胞/淋巴细胞(H/L)指标的影响进行了研究。结果表明,所有位点在试验群体中都处于Hardy-Weinberg平衡状态,5个SNPs位点的多态性都较低,只检测到两种基因型,且纯合基因型为优势等位基因。连锁不平衡分析表明SNP1和SNP2处于完美连锁状态,SNP3、SNP4和SNP5也处于完美连锁状态。单倍型分析结果表明,京星黄鸡试验群体中共检测到4种单倍型,其中单倍型Hap1 (TGACT)为优势单倍型。最小二乘分析结果表明,这5个SNPs位点不同基因型及单倍型组合对H/L的影响没有显著差异(P> 0.05)。这些结果提示以后的研究需要关注IL-2基因其他位置的SNP位点以及与更多免疫指标之间的关系,以更深入地了解IL-2在鸡免疫系统中的作用。

关键词: 鸡; IL-2基因; 异噬性粒细胞/淋巴细胞; 关联分析

Abstract: Interleukin-2(IL-2) gene was regarded as a candidate gene for immune function of Jingxinghuang chicken in this study.The genotype frequencies, allele frequencies, haplotypes and association with H/L of five SNPs were studied by direct sequencing.The results indicated that all the five loci fitted Hardy-Weinberg equilibrium and showed low degrees of heterozygosity with two genotypes detected.Meanwhile, the homozygosis genotype was predominant in all the five loci.Analysis of linkage disequilibrium (LD) suggested that SNP1 and SNP2 showed perfect LD, whereas SNP3, SNP4 and SNP5 showed perfect LD.Analysis of haplotypes revealed four haplotypes, among which Hap1 (TGACT) was predominant.The least square method (LSM) analysis showed that there were no significant difference between different genotypes and haplotype combinations(P> 0.05).The results suggested that more SNPs of IL-2 gene and association with additional immune traits would be investigated in future studies for better understand the roles in immune systems that chicken IL-2 playing.

Key words: chicken; IL-2 gene; heterophil/lymphocyte; association analysis

中图分类号:

S831

赵会静, 张敬敬, 文杰, 刘冉冉, 郑麦青, 李庆贺, 赵桂苹. 京星黄鸡白细胞介素-2基因与H/L的关联分析[J]. , 2015, 42(8): 2098-2103.

ZHAO Hui-jing, ZHANG Jing-jing, WEN Jie, LIU Ran-ran, ZHENG Mai-qing, LI Qing-he, ZHAO Gui-ping. The Association Analysis between IL-2 Gene and H/L in Jingxinghuang Chicken[J]. , 2015, 42(8): 2098-2103.

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