《中国畜牧兽医》---唯一指定的官方网站 ›› 2015, Vol. 42 ›› Issue (3): 531-536.doi: 10.11843/j.issn.1671-7236.2015.03.004

• 生物技术 • 上一篇    下一篇

沙门氏菌感染后鸡血液中核苷酸结合寡聚化结构域1基因的表达量变化分析

陶志云, 朱春红, 宋迟, 宋卫涛, 徐文娟, 姬改革, 李慧芳   

  1. 江苏省家禽科学研究所, 扬州 225125
  • 收稿日期:2014-10-23 出版日期:2015-03-20 发布日期:2015-03-30
  • 通讯作者: 李慧芳 E-mail:lhfxf-002@ailiyun.com.cn
  • 作者简介:陶志云(1979-),女,安徽凤阳人,博士生,助理研究员,研究方向:动物免疫学及遗传育种,E-mail:zhiyun2@126.com
  • 基金资助:

    国家自然科学基金青年科学基金(31101795、31101833);现代农业肉鸡产业技术体系岗位专家(CARS-42-GO3);现代农业技术领域863计划(2011AA100301)

Analysis of Expression Change of Nucleotide-binding Oligomerization Domain Containing 1 in Blood of Chickens after Salmonella Infection

TAO Zhi-yun, ZHU Chun-hong, SONG Chi, SONG Wei-tao, XU Wen-juan, JI Gai-ge, LI Hui-fang   

  1. Jiangsu Institute of Poultry Science, Yangzhou 225125, China
  • Received:2014-10-23 Online:2015-03-20 Published:2015-03-30

摘要: 为了探讨核苷酸结合寡聚化结构域1(nucleotide-binding oligomerization domain containing 1,NOD1)在抗沙门氏菌感染过程中的作用,本试验采用实时荧光定量PCR的方法检测了血液中NOD1基因在转录水平的表达量变化.试验分为3组,鸡白痢沙门氏菌组、肠炎沙门氏菌组和对照组,分别在感染后1、3、5、7 d检测NOD1 mRNA的表达水平.结果显示,感染后的1~7 d,鸡白痢沙门氏菌和肠炎沙门氏菌感染后的表达量变化趋势不同,其中,鸡白痢沙门氏菌感染后表达量呈先上升再下降然后再上升的波浪形变化,而肠炎沙门氏菌感染后的表达量呈逐渐上升趋势.与对照组相比,血液中NOD1 mRNA的水平,在鸡白痢沙门氏菌感染后的3和7 d的表达量显著高于对照组(P <0.05),在肠炎沙门氏菌感染后的5、7 d的表达量显著高于对照组(P <0.05).提示,鸡白痢沙门氏菌和肠炎沙门氏菌感染后均可促进鸡血液中NOD1基因的表达,NOD1基因可能参与了机体抗沙门氏菌感染过程.

关键词: 核苷酸结合寡聚化结构域1(NOD1); 鸡; 血液; 沙门氏菌

Abstract: In order to investigate the role of nucleotide-binding oligomerization domain containing 1 (NOD1) during the process of the Salmonella infection,the expression of NOD1 gene in blood was detected in transcriptional level using Real-time PCR method.Chickens were divided into three groups:Salmonella Pullorum group,Salmonella Enteritidis group and control group.The levels of NOD1 expression were detected 1,3,5,7 d during Salmonella challenge.The results showed that there were different changes of NOD1 expression from 1 to 7 d after two kinds Salmonella challenge.Wavy change was observed in Salmonella Pullorum group,gradually increase trend was found in Salmonella Enteritidis group.Compared with the control group,the expression levels of NOD1 mRNA in blood were significantly higher (P <0.05) at 3 and 7 d after Salmonella Pullorum infection and significantly higher (P <0.05) at 5 and 7 d after Salmonella Enteritidis infection.The results suggested that two kinds of Salmonella could promote NOD1 gene expression in blood,and NOD1 gene might be involved in the body against the Salmonella infection.

Key words: nucleotide-binding oligomerization domain containing 1 (NOD1); chicken; blood; Salmonella

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