中国畜牧兽医 ›› 2014, Vol. 41 ›› Issue (9): 140-144.

• 生理生化 • 上一篇    下一篇

松针对雏鸡免疫功能和血清胆固醇的影响

王伟1, 张红英1,2, 王学兵1, 杜芳芳2, 杜祥月1, 刘芳1   

  1. 1. 河南农业大学牧医工程学院, 河南郑州 450002;
    2. 河南省动物性食品安全重点实验室, 河南郑州 450002
  • 收稿日期:2014-03-03 出版日期:2014-09-20 发布日期:2014-09-24
  • 通讯作者: 张红英 E-mail:hongyingnd@163.com
  • 作者简介:王伟(1987- ),男,河南人,硕士,研究方向:中药抗病毒及其免疫调节作用。
  • 基金资助:

    河南省重大科技专项(111100110300);河南省高校创新人才资助项目(2012HASTIT008)。

Effect of Pine Needle on Immune Function and Serum Cholesterol of Chickens

WANG Wei1, ZHANG Hong-ying1,2, WANG Xue-bing1, DU Fang-fang2, DU Xiang-yue1, LIU Fang1   

  1. 1. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China;
    2. Henan Key Laboratory for Animal Food Safety, Zhengzhou 450002, China
  • Received:2014-03-03 Online:2014-09-20 Published:2014-09-24

摘要: 试验旨在研究松针对雏鸡免疫功能和血清胆固醇的影响。选用1日龄健康罗曼公鸡240只,随机分为4组,每组3个重复,每个重复20只鸡,对照组直接饲喂基础日粮和正常饮水,Ⅰ、Ⅱ组分别在基础日粮中以0.5、1.0 g/只剂量的松针粉拌料饲喂,Ⅲ组以0.5 g/只剂量的松针水煎液饮服。预试期4 d,正试期70 d。结果表明,Ⅰ、Ⅱ、Ⅲ组CD3+CD4+T淋巴细胞百分率在首免后第15天时高于对照组,但Ⅰ、Ⅱ组CD3+CD4+T淋巴细胞百分率在首免后第30、45、60天时则低于对照组,Ⅲ组在首免后第45天时则低于对照组;Ⅰ、Ⅱ、Ⅲ组CD3+CD8+T淋巴细胞百分率在首免后第15、30、45天时高于对照组,但Ⅰ、Ⅲ组在首免后第60天则低于对照组。Ⅰ、Ⅱ组新城疫抗体水平在首免后第14和21天时低于对照组,但在第28、35天时则高于对照组;Ⅲ组新城疫抗体水平在首免后第7、35天时低于对照组,但在第14、21天时则高于对照组。Ⅰ、Ⅱ、Ⅲ组血清总胆固醇和甘油三酯水平与对照组相比均有降低趋势,其中Ⅰ、Ⅱ组血清甘油三酯水平显著低于对照组和Ⅲ组(P<0.05);Ⅱ、Ⅲ组血清低密度脂蛋白水平低于对照组,Ⅱ组血清高密度脂蛋白水平高于对照组,但差异均不显著(P>0.05)。综上所述,松针粉和水煎剂可促进T淋巴细胞的增殖与分化,提高CD3+CD4+和CD3+CD8+T淋巴细胞含量,协同疫苗增强特异性免疫,不同程度的降低血清胆固醇水平。

关键词: 松针; 雏鸡; 免疫功能; 血清胆固醇

Abstract: The experiment was designed to study the effect of pine needle on immune function and serum cholesterol of chickens.240 healthy Roman cocks (1 day old) were randomly divided into 4 groups,3 replicates in each group and 20 chickens per replicate.The control group was fed with basal diet and normal drinking water,groups Ⅰ and Ⅱ were fed with the adding of 0.5 and 1.0 g pine needle powder,group Ⅲ was fed with pine needle decoction of 0.5 g.Pre-trial period was 4 d,formally trial period was 70 d.The results showed that the percentage of CD3+CD4+ T lymphocytes in groups Ⅰ,Ⅱ and Ⅲ were higher than that in control group at the 15 day after the first immunization,but that percentage of groups Ⅰand Ⅱ were lower than control group at the 30,45 and 60 days,Group Ⅲ was lower than control group at the 45 day.The percentage of CD3+CD8+ T lymphocytes in groups Ⅰ,Ⅱ and Ⅲ were higher than that in control group at the 15,30 and 45 days after the first immunization,but that percentage of groups Ⅰand Ⅲ were lower than control group at the 60 day.At the 14 and 21 days after the first immunization,the level of NDV antibody in groups Ⅰand Ⅱ were lower than that in control group,but it was higher than control group at the 28 and 35 days.The level of NDV antibody in group Ⅲ was higher than that in control group at the 14 and 21 days while it was lower at the 7 and 35 days.The levels of serum total cholesterol and triglyceride in groups Ⅰ,Ⅱ and Ⅲ had lower trend compared with control group,and the level of serum triglyceride in groups Ⅰand Ⅱ was significantly lower than that in control group and group Ⅲ (P<0.05).The level of serum low density lipoprotein in groups Ⅱ and Ⅲ was lower than that in control group,while the level of serum high density lipoprotein in group Ⅱ was higher than that in control group,but there were no significant differences (P>0.05).In conclusion,the pine needle could promote the proliferation and differentiation of T lymphocytes,improve the content of CD3+CD4+ and CD3+CD8+ lymphocyte,collaborate with vaccine to enhance the specific immune,and reduce the serum cholesterol level in different degrees.

Key words: pine needle; chicken; immune function; serum cholesterol

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