miR-138对小鼠乳腺发育及泌乳功能的影响

›› 2014, Vol. 41 ›› Issue (11): 223-227.

miR-138对小鼠乳腺发育及泌乳功能的影响

李丹, 王杰, 谢雪娇, 李庆章, 王春梅

东北农业大学乳品科学教育部重点实验室, 黑龙江哈尔滨 150030

收稿日期:2014-03-13 出版日期:2014-11-20 发布日期:2014-12-06
通讯作者: 王春梅 E-mail:wangcm-1@163.com
作者简介:李丹(1988-),女,黑龙江人,硕士生,主要从事miRNA对乳腺发育与泌乳调控作用的研究。
基金资助:
黑龙江省教育厅重点项目(1154z1004);东北农业大学人才项目(2009)。

Effect of miR-138 on Development and Lactation in Mouse Mammary Gland

LI Dan, WANG Jie, XIE Xue-jiao, LI Qing-zhang, WANG Chun-mei

Key Laboratory Dairy Science of Education Ministry, Northeast Agricultural University, Harbin 150030, China

Received:2014-03-13 Online:2014-11-20 Published:2014-12-06

摘要/Abstract

摘要： microRNAs(miRNAs)在各种类型细胞增殖、分化和凋亡中发挥了重要作用。miR-138参与乳腺发育周期过程中细胞增殖分化的调控。试验以小鼠为动物模型,尾静脉注射miR-138基因抑制剂,应用幼鼠体重称重法,检测miR-138抑制后乳腺泌乳量变化;应用电子显微镜等技术观测乳腺组织形态变化,抑制miR-138后,小鼠乳腺上皮细胞增加,乳汁分泌量增加;抑制miR-138后,观察小鼠乳腺组织超微结构可发现,乳腺细胞的代谢活动增强;收集尾静脉注射miR-138 抑制剂的小鼠乳汁,检测发现其乳糖、乳中酪蛋白含量均有所增高。研究认为,miR-138可刺激乳腺上皮细胞增殖,增加乳腺发育泌乳过程中乳的分泌,并且调控乳汁中重要成分含量。

关键词: miR-138; 乳腺发育; 泌乳

Abstract: microRNAs (miRNAs) play a key role in cell proliferation,differentiation and apoptosis. miRNA involved in the regulation of mammary gland development in cell proliferation and differentiation. In this experiment, we used mice as an animal model to study the role of miR-138 in mouse mammary gland development and lactation. Maternal tail vein injection of miR-138 inhibitors, pups weighing method applied to detect the changes of milk yield;electron microscopy techniques was used to observe ultrastructure changes in mammary gland tissue. The results showed that after intravenous injection of miR-138 inhibitor, female mouse increased maternal mammary epithelial cells, increasing the amount of milk secretion,ultrastructure of cells found in the metabolic activity,collect female mouse milk after miR-138 inhibitor, detect lactose and casein of the milk were all increased. The results suggested that the inhibition of miR-138 could stimulate mammary epithelial cell proliferation, increase the secretion of milk, and regulation of the ingredient of the milk.

Key words: miR-138; mammary gland development; lactation

中图分类号:

李丹, 王杰, 谢雪娇, 李庆章, 王春梅. miR-138对小鼠乳腺发育及泌乳功能的影响[J]. , 2014, 41(11): 223-227.

LI Dan, WANG Jie, XIE Xue-jiao, LI Qing-zhang, WANG Chun-mei. Effect of miR-138 on Development and Lactation in Mouse Mammary Gland[J]. , 2014, 41(11): 223-227.

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