关键词: 混合感染; 诊断; 防治

Abstract: In order to diagnose the cause of disease and death of Tibetan pigs,PCR,RT-PCR and Real-time PCR methods were used in this experiment combined with epidemiological investigation,clinical diagnosis,autopsy pathology and other laboratory tests,the results showed that congestion and hemorrhage of heart and lung,lung became meat,trachea filled with white foam,systemic lymph hemorrhage of Tibetan pig,Real-time PCR detected porcine circovirus type 2 positive,PCR detected porcine pseudorabies virus positive,there was no Pleuropneumoniae actinobacillus and Mycoplasma hyopneumoniae strips,RT-PCR did not amplify the strips of classical swine fever virus and porcine reproductive and respiratory syndrome virus,staining microscopy showed there was Mycoplasma suis. The results showed the diseased pig was mixed infections with porcine pseudorabies,porcine circovirus disease and porcine eperythrozoonosis,after integrated control,the epidemic situation had been controlled. Diagnosis and treatment of the case might provide effective prevention and treatment methods and experiences for mixed infections by porcine pseudorabies,porcine circovirus disease and porcine eperythrozoonosis.

Key words: mixed infections; diagnosis; prevention and treatment