过氧化物酶体增殖物激活受体α在猪肝脏组织中发育性表达研究

›› 2014, Vol. 41 ›› Issue (10): 178-182.

过氧化物酶体增殖物激活受体α在猪肝脏组织中发育性表达研究

徐凯¹, 王效京², 刘宏³, 郭晓红¹, 高鹏飞¹, 曹果清¹, 李步高¹

1. 山西农业大学动物科技学院, 山西太谷 030801;
2. 山西省畜禽繁育工作站, 山西太原 030001;
3. 大同市种猪场, 山西大同 037000

收稿日期:2014-03-10 出版日期:2014-10-20 发布日期:2014-10-29
通讯作者: 李步高 E-mail:jinrenn@163.com
作者简介:徐凯(1989－),男,山西人,硕士,研究方向:分子遗传学与动物育种。
基金资助:
山西省科技创新重点团队项目(2013131019);山西省科技攻关项目(20080311031、20130311026-1);山西省科技成果推广项目(2012072008)

Study on Developmental Expression of PPARα mRNA in Liver Tissues of Pig

XU Kai¹, WANG Xiao-jing², LIU Hong³, GUO Xiao-hong¹, GAO Peng-fei¹, CAO Guo-qing¹, LI Bu-gao¹

1. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China;
2. Shanxi Livestock and Poultry Breeding Station, Taiyuan 030001, China;
3. Datong Pig Breeding Farm, Datong 037000, China

Received:2014-03-10 Online:2014-10-20 Published:2014-10-29

摘要/Abstract

摘要： 为了探究过氧化物酶体增殖物激活受体α(peroxisome proliferator activated receptor alpha,PPARα)基因在不同脂肪型猪肝脏组织中的发育表达规律,本研究采用实时荧光定量PCR方法,检测了马身猪和大白猪0、1、2、3、4、5和6月龄个体肝脏内PPARα基因mRNA的表达量。结果显示,马身猪和大白猪肝脏组织PPARα基因mRNA发育性变化趋势存在差异,大白猪PPARα 基因表达量呈现出前、后期高中期低的变化趋势,0月龄时表达量最高,3月龄时表达量最低,两者差异极显著(P<0.01),而马身猪表达量呈现前期高后期低的变化趋势,0月龄和1月龄的表达量极显著高于其他月龄(P<0.01)。上述结果揭示猪PPARα可能是肝脏脂质代谢的调控因子,为猪脂肪沉积的调控机制提供理论数据。

关键词: 猪; PPARα基因; mRNA; 发育性表达

Abstract: To investigate the developmental expression patterns of PPARα gene in liver of different lard type pigs in this study,we detected the PPARα expression changes of mRNA at the ages of 0,1,2,3,4,5 and 6 months in the livers of Mashen pigs and Large White pigs by qRT-PCR. This results showed that the expression of PPARα mRNA in liver exhibited specific developmental changes and different patterns between the two pig breed. At the same time,developmental expression patterns of PPARα mRNA in Large White pigs established the higher in adjacent periods,the highest in 0 month,but the lowest in 3 month,which showed highly significant between the two monthes (P<0.01),while the higher in the early period and the lower in the later period for Mashen pigs,the expression level in 0 and 1 months was significantly higher than that of other months (P<0.01). The results revealed that PPARα was a regulatory factor involved in the liver lipid metabolism,which provided the theory data for the regulatory mechanism of fat deposition in pigs.

Key words: pig; PPARα gene; mRNA; developmental expression

中图分类号:

Q786

徐凯, 王效京, 刘宏, 郭晓红, 高鹏飞, 曹果清, 李步高. 过氧化物酶体增殖物激活受体α在猪肝脏组织中发育性表达研究[J]. , 2014, 41(10): 178-182.

XU Kai, WANG Xiao-jing, LIU Hong, GUO Xiao-hong, GAO Peng-fei, CAO Guo-qing, LI Bu-gao. Study on Developmental Expression of PPARα mRNA in Liver Tissues of Pig[J]. , 2014, 41(10): 178-182.

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