奶牛瘤胃pH不同测定方法的比较研究

›› 2013, Vol. 40 ›› Issue (11): 213-216.

奶牛瘤胃pH不同测定方法的比较研究

赵小伟^1,2, 王加启¹, 卜登攀¹, 马露¹, 徐晓燕¹, 安朋朋¹, 周凌云¹

1. 中国农业科学院北京畜牧兽医研究所, 动物营养学国家重点实验室, 北京 100193;
2. 安徽农业科学院畜牧兽医研究所, 安徽合肥 230031

收稿日期:2012-11-15 出版日期:2013-11-20 发布日期:2013-12-19
通讯作者: 王加启 E-mail:wang-jia-qi@263.com
作者简介:赵小伟(1985-),男,安徽人,硕士,研究方向:反刍动物营养。
基金资助:
"十二五"科技支撑计划"奶牛营养调控与粗饲料高效利用关键技术研究"(2012BAD12B02);现代农业产业技术体系专项资金(nycytx-04-01);动物营养学国家重点实验室自主研究课题(2004DA125184G1003)。

Comparative Study on Two Different Test Methods in Rumen pH of Dairy Cows

ZHAO Xiao-wei^1,2, WANG Jia-qi¹, BU Deng-pan¹, MA Lu¹, XU Xiao-yan¹, AN Peng-peng¹, ZHOU Ling-yun¹

1. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. Institute of Animal Husbandry and Veterinary Sciences, Anhui Academy of Agricultural Sciences, Hefei 230031, China

Received:2012-11-15 Online:2013-11-20 Published:2013-12-19

摘要/Abstract

摘要： 本试验旨在研究奶牛瘤胃pH不同测定方法的实际效果。选用4头健康且装有永久性瘤胃瘘管的中国黑白花荷斯坦奶牛,对传统便携式pH计(MANpH)和连续pH记录无线装置(LRCpH)2种不同瘤胃内容物pH检测效果进行了比较研究。结果表明,MANpH和LRCpH 2种方法测定的瘤胃内容物pH差异不显著(P>0.05),瘤胃内容物pH 24 h变化亦无显著性差异(P>0.05)。提示,LRCpH无线装置不仅可准确测定瘤胃内容物pH,且能实时连续监测奶牛采食后瘤胃内pH的动态变化。

关键词: 便携式pH计; pH记录无线装置; pH; 奶牛

Abstract: The experiment was carried out to compare the real impression between two different text methods in rumen pH.Four multiparous Chinese Holstein cows fitted with rumen cannulas were used to compare two different test methods in rumen pH of dairy cows,the two methods were simple pH meter (MANpH) and continuous recording wireless equipment (LRCpH).The results showed that there were no significant differences between two different test methods in rumen pH of dairy cows(P>0.05).Meanwhile,there were also no significant differences in 24 h observed value between MANpH and LRCpH(P>0.05).This experiment indicated that LRCpH wireless equipment not only could test rumen pH,but also could Real-time and continuous record the rumen pH after feeding.

Key words: simple pH meter; continuous recording wireless equipment; pH; dairy cow

中图分类号:

S823

赵小伟, 王加启, 卜登攀, 马露, 徐晓燕, 安朋朋, 周凌云. 奶牛瘤胃pH不同测定方法的比较研究[J]. , 2013, 40(11): 213-216.

ZHAO Xiao-wei, WANG Jia-qi, BU Deng-pan, MA Lu, XU Xiao-yan, AN Peng-peng, ZHOU Ling-yun. Comparative Study on Two Different Test Methods in Rumen pH of Dairy Cows[J]. , 2013, 40(11): 213-216.

参考文献

1 王加启,李树聪,魏宏阳,等.实时连续监测反刍动物瘤胃液pH值的装置:中华人民共和国,CN2836723[P].2006.
2 冯仰廉.反刍动物营养学[M].北京:科学出版社,2004.
3 崔莲花,严昌国,Kim Hyen,等.发芽玉米对荷斯坦奶牛瘤胃发酵、产奶性能及乳品质的影响[J].中国畜牧兽医,2012,39(11):94~99.
4 Cottee G,Kyriazakis I,Widowski T M,et al.The effects of subacute ruminal acidosis on sodium bicarbonate supplemented water intake for lactating cows[J].Journal of Dairy Science,2004,87:2248~2253.
5 Dado R G,Allen M S.Continuous computer acquisition of feed and water intakes,chewing,reticular motility,and ruminal pH of cattle[J].Journal of Dairy Science,1993,76:1589~1600.
6 Duffield T,Plaizier J C,Fairfield A,et al.Comparison of techniques for measurement of rumen pH in lactating dairy cows[J].Journal of Dairy Science,2004,87:59~66.
7 Graf C M,Kreuzer M,Dohme F.Effects of supplemental hay and corn silage versus full-time grazing on ruminal pH and chewing activity of dairy cows[J].Journal of Dairy Science,2005,88:711~725.
8 Garrett E F,Pereira M N,Nordlund K N,et al.Diagnostic methods for the detection of subacute ruminal acidosis in dairy cows[J].Journal of Dairy Science,1999,82:1170~1178.
9 Keefe G P,Ogilvie T H.Comparison of oro-ruminal probe and rumenocentesis for prediction of rumen pH in dairy cattle[A].In proceedings of the annual conference[C].1997.
10 Keunen J E,Plaizier J C,Kyriazakis L,et al.Effects of a subacute ruminal acidosis model on the diet selection of dairy cows[J].Journal of Dairy Science,2002,12:3304~3313.
11 Kononoff P J,Heinrichs A J,Lehman H A.The effect of corn silage particle size on eating behavior,chewing activities,and rumen fermentation in lactating dairy cows[J].Journal of Dairy Science,2003,86:3343~3353.
12 Nocek J E,Allman J G,Kautz W P.Evaluation of an indwelling ruminal probe methodology and effect of grain level on diurnal pH variation in dairy cattle[J].Journal of Dairy Science,2002,85:422~428.
13 Nordlund K V,Garrett E F.Rumenocentesis: A technique for the diagnosis of subacute rumen acidosis in dairy herds[J].Bovine Pract,1994,28:109~112.
14 Oetzel G R,Nordlund K V.Methods of evaluating ruminal pH in dairy cattle[A].In proceedings of the annual conference.1998.
15 Penner G B,Beauchemin K A,Mutsvangwa T,et al.An evaluation of the accuracy and precision on stand-alone summersible continuous ruminal pH measurement system[J].Journal of Dairy Science,2006,89:2132~2140.
16 Reis R B,Combs D K.Effects of increasing levels of grain supplementation on rumen environment and lactation performance of dairy cows grazing grass-legume pasture[J].Journal of Dairy Science,2000,83:2888~2898.
17 Rustomo B,Alzahal O,Cant J P,et al.Acidogenic value of feeds.Ⅱ. Effects of rumen acid load from feeds on dry matter intake,ruminal pH,fiber degradability and milk production in the lactating dairy cow[J].Canadian Journal of Animal Science,2006,86:119~126.

[1]	樊艺萌, 魏媛媛, 王惠茹, 尕玉, 张艳楠, 赵卿宇, 郝智慧. 基于网络药理学与分子对接探究中兽药复方乳康颗粒治疗奶牛乳房炎的作用机制[J]. 中国畜牧兽医, 2023, 50(6): 2507-2517.
[2]	胡红莲, 李超, 陈利青, 宋利文, 赵濛, 张剑霞, 王典, 高民. β-胡萝卜素对奶牛泌乳高峰期产乳性能及血常规指标、血清生化和免疫指标的影响[J]. 中国畜牧兽医, 2023, 50(4): 1375-1384.
[3]	刘鑫煜, 崔强, 张兰欣, 范佺荣, 李政忆, 高瑞峰. 基于网络药理学和分子对接探究诃子鞣酸防治奶牛子宫内膜炎的作用机制[J]. 中国畜牧兽医, 2023, 50(4): 1632-1641.
[4]	李丹, 张海森, 王逸群, 高登科, 赵泓淙, 李超, 靳亚平, 陈华涛. 奶牛CRY1基因真核表达载体构建、生物信息学分析及组织表达谱研究[J]. 中国畜牧兽医, 2023, 50(3): 870-881.
[5]	孔维怡, 王菲菲, 岳康, 秦俊杰, 朱浩, 郭延生. 基于UPLC-MS/MS代谢组学技术分析归芪益母口服液对产后奶牛血浆代谢物的影响[J]. 中国畜牧兽医, 2023, 50(3): 913-923.
[6]	钟华晨, 王丽芳, 郭晨阳, 刘嘉琳, 宋洁. 内蒙古地区乳房炎奶样与环境中细菌的分离鉴定及耐药性分析[J]. 中国畜牧兽医, 2023, 50(2): 817-826.
[7]	贺越, 王辉, 陈睿鹏, 余志雪, 唐湘方, 呙于明, 熊本海. 光学和电化学生物传感器对奶牛重要疾病诊断的研究进展[J]. 中国畜牧兽医, 2023, 50(2): 827-837.
[8]	付琪, 余厚默, 张海亮, 许静漪, 胡羽飏, 段禹交, 周月玲, 黄思琪, 张亚一, 郭刚, 俞英, 王雅春. 荷斯坦奶牛牛嘴宽度影响因素分析及遗传参数估计[J]. 中国畜牧兽医, 2023, 50(1): 230-237.
[9]	孙愉, 张昊, 李畅洋, 陶燕子, 王秋菊. 牛源抗氧化芽孢杆菌的分离鉴定及生物特性研究[J]. 中国畜牧兽医, 2023, 50(1): 359-367.
[10]	纪皓楠, 高清, 常瑶, 张海亮, 吴宏军, 柴河, 俞英, 王雅春. 荷斯坦牛泌乳速度指标影响因素分析[J]. 中国畜牧兽医, 2022, 49(9): 3475-3483.
[11]	邵明珠, 赵允清, 李松励, 侯绍华, 张艳英. 河北省奶牛源大肠杆菌的分离鉴定、致病性及中草药体外抑菌效果研究[J]. 中国畜牧兽医, 2022, 49(9): 3683-3691.
[12]	蔡萌, 朱晓艳, 王梦玲, 刘子豪, 熊本海, 杨亮. 金黄色葡萄球菌处理对奶牛乳腺上皮细胞外泌体表征的影响[J]. 中国畜牧兽医, 2022, 49(8): 3091-3098.
[13]	鲁文赓, 司琳清, 田春雨, 袁思琦, 谢何昕, 周继伟, 金吉东, 韩英浩. 奶牛子宫内膜上皮细胞氧化应激模型的建立及奶牛脂肪间充质干细胞对其迁移的影响[J]. 中国畜牧兽医, 2022, 49(7): 2506-2514.
[14]	李尚汝, 宋佳美, 张城瑞, 孙雨坤, 张永根. 基于机器学习算法的奶牛疾病预测模型的研究[J]. 中国畜牧兽医, 2022, 49(7): 2534-2546.
[15]	许晓玲, 白佳桦, 肖霖力, 秦玉圣, 宋玉清, 刘彦. 复合添加剂对解冻后奶牛性控冻精质量及受胎率的影响[J]. 中国畜牧兽医, 2022, 49(7): 2654-2660.