鸡肠道大肠杆菌的分离鉴定及耐药性分析

›› 2013, Vol. 40 ›› Issue (11): 179-184.

鸡肠道大肠杆菌的分离鉴定及耐药性分析

张俊丰¹, 黄东璋², 陈琳², 刘俊栋², 魏冬霞², 管远红², 丁小丽², 李巨银², 颜友荣², 周思思², 陈新秀²

1. 重庆市畜牧科学研究院, 重庆荣昌 402460;
2. 江苏农牧科技职业学院, 江苏泰州 225300

收稿日期:2013-03-27 出版日期:2013-11-20 发布日期:2013-12-19
通讯作者: 陈琳 E-mail:chenlin20086666@126.com
作者简介:张俊丰(1969-),男,四川人,学士,兽医师,研究方向:畜禽疫病防治。
基金资助:
江苏省自然科学基金(BK2008587);江苏农牧科技职业学院重点项目(ZD1202)。

Identification and Drug Resistance Analysis of Chicken Intestinal Escherichia coli

ZHANG Jun-feng¹, HUANG Dong-zhang², CHEN Lin², LIU Jun-dong², WEI Dong-xia², GUAN Yuan-hong², DING Xiao-li², LI Ju-yin², YAN You-rong², ZHOU Si-si², CHEN Xin-xiu²

1. Chongqing Academy of Animal Science, Rongchang 402460, China;
2. Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, China

Received:2013-03-27 Online:2013-11-20 Published:2013-12-19

摘要/Abstract

摘要： 为分析鸡肠道大肠杆菌对常用抗菌药物的耐药性,试验采集了34个鸡直肠拭子样本,分离并鉴定大肠杆菌,采用微量稀释法测定分离菌对卡那霉素、氯霉素、氨苄西林、环丙沙星、头孢曲松钠、庆大霉素、磺胺嘧啶和强力霉素8种药物的敏感性。结果显示,分离并鉴定出34株鸡肠道大肠杆菌,其中4株经鉴定为产H₂S大肠杆菌。34株菌对卡那霉素、氯霉素、氨苄西林、环丙沙星、头孢曲松钠、庆大霉素、磺胺嘧啶和强力霉素的MIC₅₀分别为>256、256、>256、16、<0.125、256、>512和16 μg/mL,耐药率依次为100.0%、100.0%、100.0%、70.6%、11.8%、94.1%、100.0%和82.3%。34株菌均为多重耐药菌株,最少可对5种药物耐药,最多可对8种药物耐药,其中耐8种药物菌株百分率为2.9%;耐7种药物菌株百分率为58.8%;耐6种药物菌株百分率为32.4%;耐5种药物菌株百分率为5.9%。试验结果表明,34株鸡肠道大肠杆菌对8种常用抗菌药物的耐药谱广、耐药率高,兽医临床应根据试验结果调整用药方案。

关键词: 鸡; 产H₂S大肠杆菌; 鉴定; 耐药

Abstract: Total of 34 chicken rectal swab samples were collected to isolate and identify Escherichia coli. And all isolates were tested for susceptibility to 8 antimicrobial agents by using the broth micro-dilution method. As a result, 34 intestinal Escherichia coli isolates recovered from chicken were identified and 4 of them were hydrogen sulfide producing stains. Their MIC₅₀ to kanamycin, chloramphenicol, ampicillin, cyprofloxacin, ceftriaxone sodium, gentamicin, sulfadiazine and doxycycline were more than 256 μg/mL, equal to 256 μg/mL, more than 256 μg/mL, equal to 16 μg/mL, less than 0.125 μg/mL, equal to 256 μg/mL, more than 512 μg/mL and equal to 16 μg/mL,respectively. And their resistant rates to these 8 antimicrobial agents were ordered as 100.0%, 100.0%, 100.0%, 70.6%, 11.8%, 94.1%, 100.0% and 82.3%, respectively. All isolates were multi-drug resistant strains. The minimal and maximum kinds of antimicrobial agents to 34 Escherichia coli strains were 5 and 8, respectively. The percentages of resistant isolates against 5, 6, 7 and 8 antimicrobial agents were ordered as 5.9%, 32.4%, 58.8% and 2.9%.It was suggested that wide resistant spectrum and high resistant ratio were presented in 34 intestinal Escherichia coli isolates. Medicinal proposals in clinical should be adjusted according to the antimicrobial susceptibility test.

Key words: chicken; hydrogen sulfide producing Escherichia coli; identification; resistance

中图分类号:

S859.7

张俊丰, 黄东璋, 陈琳, 刘俊栋, 魏冬霞, 管远红, 丁小丽, 李巨银, 颜友荣, 周思思, 陈新秀. 鸡肠道大肠杆菌的分离鉴定及耐药性分析[J]. , 2013, 40(11): 179-184.

ZHANG Jun-feng, HUANG Dong-zhang, CHEN Lin, LIU Jun-dong, WEI Dong-xia, GUAN Yuan-hong, DING Xiao-li, LI Ju-yin, YAN You-rong, ZHOU Si-si, CHEN Xin-xiu. Identification and Drug Resistance Analysis of Chicken Intestinal Escherichia coli[J]. , 2013, 40(11): 179-184.

参考文献

1 丁业荣, 杨杰,范玉珍,等.产H₂S肠道致病性大肠埃希菌O128:K67的发现与研究[J].安徽卫生职业技术学院学报,2003(5):85~88.
2 于阿芳.鸡致病性大肠杆菌的药敏试验、血清型鉴定及耐药基因的PCR检测[D].泰安:山东农业大学,2009.
3 王宇,王永芬.鸡大肠杆菌病的流行病学研究[J].信阳师范学院学报(自然科学版),2007,20(3):317~340.
4 王兵,芮艺.鸡大肠杆菌地区菌株的分离鉴定与药敏试验[J].家畜生态学报,2006,27(2):73~75.
5 朱子洁,程丽华,黄阳.鸡大肠杆菌病的病原菌生物学特性[J].兽医导刊,2009,144(8):25~26.
6 张小林.健康人群肠道大肠杆菌耐药状况[J].国外医学流行病学传染病学分册,1996,23(2):79~81.
7 张秀英,吴好庭,赵晖. 部分鸡场大肠杆菌对抗菌药物的耐药性[J].中国兽医学报,2005,25(4):422~424.
8 张俊丰,陈琳,魏冬霞,等.92株猪腹泻大肠杆菌的耐药性分析[J].中国畜牧兽医,2010,37(11):153~156.
9 陈建辉,谢一俊,郭维植,等.从健康带菌者粪便中检出3株产H₂S大肠埃希氏菌的报道[J].预防医学文献信息,2003(3):356~357.
10 郝智慧,肖希龙,邱梅,等.不同区域鸡大肠杆菌对抗菌药的耐药性比较[J].中国兽医科学,2009,39(1): 84~88.
11 恽时锋,潘震寰,郑明球.鸡大肠杆菌病研究进展[J].畜牧与兽医,1998,30(6):277~280.
12 徐树强,杨安龙,吴兆林.扬州地区鸡大肠杆菌的分离鉴定与药敏试验[J].江苏农业科学,2006(6):330~332.
13 常宏伟,赵俊,丁业荣,等.17株产H₂S致泻大肠埃希菌的微生物学研究[J].中华疾病控制杂志,2009(3):320~323.
14 常虹,薛镧.粪便标本分离出1株产H₂S肠致病性大肠埃希菌[J].预防医学情报杂志,2004(2):203.
15 塞弗. 苏敬良,高福,索勋,主译.禽病学[M].第11版.北京:中国农业出版,2005.
16 Bonten M, Stobberingh E, Philips J, et al. High prevalence of antibiotic resistant Escherichia coli infaecal samples of students in the southeast of the Netherlands[J].the Journal of Antimicrobial Chemot-herapy,1990,26(4): 585~592.
17 Borgen K, Simonsen G S, Sundsfjord A,et al. Continuing high prevalence of VanA type vancomycin resistant enterococci on Norwegian poultry farms three years after avoparcin was banned[J].Journal of Applied Microbiology,2000,89(3):478~485.
18 Darland G, Davis B R. Biochemical and serological characterization of hydrogen sulfide-positive variants of Escherichia coli[J]. Applied Microbiology, 1974, 27(1):54~58.
19 Enne V I,Livermore D M,Stephens P, et al. Persistence of sulphonamide resistance in Escherichia coli in the UK despite national prescribing restriction[J].Lancet, 2001, 357(9265):1325~1328.
20 Heuer O E,Pedersen K,Jensen L B, et al. Persistence of vancomycin resistant enterococci (VRE) in broiler houses after the avoparcin ban[J]. Microbial Drug Resistance,2002,8(4):355~361.
21 Jones R T,Thai L P,Silver R P. Genetic and molecular characterization of an Escherichia coli plasmid coding for hydrogen sulfide production and drug resistance[J].Antimicrobial Agents and Chemotherapy,1978,14(5):765~770.
22 Lautrop H, Orskov I, Gaarslev K. Hydrogensulphide producing variants of Escherichia coli[J]. Acta Pathologica Microbiologica Scandinavica Section B Microbiology and Immunology,1971,79(5):641~650.
23 Lim S K,Lee H S,Nam H M,et al. Antimicrobial resistance observed in Escherichia coli strains isolated from fecal samples of cattle and pigs in Korea during 2003—2004[J].International Journal of Food Microbiology,2007,116(2):283~286.
24 Magalhaes M,Vance M. Hydrogen sulphide-positive strains of Escherichia coli from swine[J].Journal of Medical Microbiology, 1978,11(2):211~214.
25 Maker M D,Washington J A.Hydrogen sulfide-producing variants of Escherichia coli[J].Appllied and Environmental Microbiology,1974,28(2):303~305.
26 Orskov I,Orskov F. Plasmid determined H₂S character in Escherichia coli and its relation to plasmid-carried rafikose fermentation and tetracycline resistance characters[J].Journal of General Microbiology,1973,77(2):487~499.
27 Pupo G M,Karaolis D K,Lan R,et al. Evolutionary relationships among pathogenic and nonpathogenic Escherichia coli strains inferred from multilocus enzyme electrophoresis and mdh sequence studies[J]. Infection and Immunity,1997,65(7):2685~2692.

[1]	杜元军, 段国超, 刘玮, 李大鹏, 韩海霞, 雷秋霞, 周艳, 刘杰, 王杰, 曹顶国, 李福伟, 陈甫. 暗斑对蛋鸡蛋品质的影响及其与肠道功能的关系[J]. 中国畜牧兽医, 2023, 50(6): 2305-2311.
[2]	吴立婷, 陆睿, 刘半红, 包红朵, 王永娟, 周艳, 王冉, 张辉. 产气荚膜梭菌噬菌体及乳酸杆菌联用改善肉鸡生长性能和调节肠道菌群功能研究[J]. 中国畜牧兽医, 2023, 50(6): 2321-2332.
[3]	卢玉葵, 刘佳琪, 钟嘉诚, 陈济铛, 朱婉君, 张溢珊, 张济培. 鹅源副鸡禽杆菌的分离鉴定及致病性研究[J]. 中国畜牧兽医, 2023, 50(6): 2562-2572.
[4]	陈建师, 张宁, 浦方雄, 苗健佳, 曾楠, 李炳学. 炭疽芽孢杆菌携带前噬菌体的预测及耐药性与毒力分析[J]. 中国畜牧兽医, 2023, 50(6): 2573-2584.
[5]	片慧芳, 杜旭彬, 李妍, 张雨辰, 刘斐, 虞德兵. 海兰褐壳蛋鸡小黄卵泡颗粒细胞全长转录组测序分析[J]. 中国畜牧兽医, 2023, 50(5): 1754-1763.
[6]	王浩睿, 禹保军, 杨嘉翊, 魏榕, 付希, 王川川, 张迪, 李德生, 蔡正云, 顾亚玲, 张娟. 静原鸡DERA基因生物信息学分析及组织表达研究[J]. 中国畜牧兽医, 2023, 50(5): 1764-1773.
[7]	刘佳隆, 张译文, 张悦然, 孙奇娟, 陈建, 何雷, 贾艳艳, 丁轲, 余祖华. 鸡ELAVL1基因克隆、原核表达及生物信息学分析[J]. 中国畜牧兽医, 2023, 50(5): 1807-1817.
[8]	乔宏兴, 张菡, 赵圣振, 孙彦婷, 张晓静, 康宇, 边传周. 发酵杜仲叶对肉鸡生产性能、抗氧化性能和免疫相关基因表达的影响[J]. 中国畜牧兽医, 2023, 50(5): 1845-1854.
[9]	吴静璇, 孙征, 马雪莲, 张丽阳, 廖秀冬, 罗绪刚, 李素芬, 吕林. 中国畜禽饲料资源中镁含量的调查研究[J]. 中国畜牧兽医, 2023, 50(5): 1888-1898.
[10]	谢华亮, 雷小文, 李建军, 刘珍妮, 钟云平, 谭东海, 张强. 蚯蚓液对105~175日龄蛋鸡产蛋性能、血清生化指标、抗氧化指标及免疫相关指标的影响[J]. 中国畜牧兽医, 2023, 50(5): 1899-1906.
[11]	曾成容, 刘馨, 毕文文, 梅世慧, 何广霞, 张俊杰, 文明, 周碧君, 陈江凤, 姜海波. 嗜水气单胞菌群体感应系统及其抑制剂应用研究进展[J]. 中国畜牧兽医, 2023, 50(5): 1991-2000.
[12]	张金武, 魏紫, 冀禹彤, 吴昌旭, 李振民, 韦英益, 胡庭俊, 于美玲. A型产气荚膜梭菌诱导黄羽肉鸡肠道氧化应激和炎性损伤研究[J]. 中国畜牧兽医, 2023, 50(5): 2069-2081.
[13]	蒋山翊, 周碧君, 文明, 王开功. 中药联合复合益生菌对鸡柔嫩艾美耳球虫的预防作用[J]. 中国畜牧兽医, 2023, 50(5): 2092-2102.
[14]	刘歆, 陈群, 邱玉朗, 李林, 侯国喜, 闫晓刚, 李忠和, 陈龙, 高星爱, 柳冬梅, 翟迪, 闫秋良. 鸡粪堆肥含氮和含硫废气产生机制及控制技术研究进展[J]. 中国畜牧兽医, 2023, 50(5): 2166-2174.
[15]	李梦媛, 刘梦潜, 康佳佳, 黄英, 段勇, 贾俊静, 葛长荣, 曹玮娜. 胍基乙酸对肉鸡肉品质和腹脂沉积的影响[J]. 中国畜牧兽医, 2023, 50(4): 1385-1394.