遗传背景和胎儿日龄对猪类胚胎生殖细胞培养的影响

›› 2013, Vol. ›› Issue (7): 137-142.

遗传背景和胎儿日龄对猪类胚胎生殖细胞培养的影响

董晓¹, 冯书堂², 王红军¹

1. 青岛农业大学生命科学学院, 山东青岛 266109;
2. 中国农业科学院北京畜牧兽医研究所, 北京 100193

收稿日期:2012-12-04 出版日期:2013-07-20 发布日期:2013-07-24
通讯作者: 王红军,冯书堂 E-mail:hjwang11@hotmail.com;fst508@sina.com
作者简介:董晓(1969-),女,山东人,博士,副教授,研究方向:生殖生理。
基金资助:
青岛农业大学博士启动基金(630319);青岛农业大学高层次人才科研基金(631114)。

The Effect on the Culturing of Porcine Embryonic Germ Cells by Genetic Background and Fetal Stage

DONG Xiao¹, FENG Shu-tang², WANG Hong-jun¹

1. College of Life Science, Qingdao Agricultural University, Qingdao 266109, China;
2. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received:2012-12-04 Online:2013-07-20 Published:2013-07-24

摘要/Abstract

摘要： 本试验研究了遗传背景和胎儿日龄对猪类胚胎生殖细胞(embryonic germ cells,EG)培养的影响,分别利用五指山猪(WZSP)和微型白猪胎儿为试验材料,比较了2种猪胎儿在EG培养方面的异同。试验结果表明,在相同条件下,2种猪均得到了可以传代的EG细胞集落,得到的EG集落AP染色呈阳性,体外培养能形成类胚体,类胚体继续培养能分化成多种细胞。试验比较了5个指标:平均第1次出现EG集落时间(T1)、第1次出现大批EG集落时间(T2)、第1次传代时间(T3)、平均传代间隔(T4)及传代情况(P),结果显示,WZSP和微型白猪2个品系差异不显著(P>0.05),表明WZSP和微型白猪都可以用来作为猪EG细胞建系的材料;分别采集第26、27、28天胎儿比较日龄对EG培养的影响。试验测量了不同日龄胎儿中肾大小,比较以上5个指标,观察胎儿日龄对建立猪EG细胞系方面的影响,结果表明第26、27、28天胎儿在EG培养方面差异不显著(P>0.05)。

关键词: 遗传背景; 胎儿日龄; 猪; 胚胎干细胞

Abstract: Factors on culturing of embryonic gem cells (EG)in pigs were studied in order to establish porcine EG lines. In order to analysis genetic background influencing the culturing of the porcine embryonic germ cells, fetuses were collected from Wuzhishan pigs (WZSP) and White Minitype pigs respectively. Under the same culturing conditions, when the first time of EG colonies coming forth (T1),the first time of large quantities of EG colonies coming forth (T2),the first time of passage (T3),average passage intervals (T4),passages (P) were considered. The results showed that there was no notable difference in EG culturing between the two types of pigs(P>0.05). That was WZSP and White Minitype pigs were all suitable materials to establish porcine EG lines; In order to analysis fetal stages influencing the culturing of porcine EG, fetuses at 26 d, 27 d and 28 d were collected respectively, the length of the fetuses and the length of the fetal mesonephros were measured, the 5 indexes above mentioned were still considered, the results showed that under the same conditions, there was no notable difference in EG culturing using fetuses collected on 26 d, 27 d and 28 d respectively(P>0.05).

Key words: genetic background; fetal stage; pig; embryonic germ cells

中图分类号:

S828

董晓, 冯书堂, 王红军. 遗传背景和胎儿日龄对猪类胚胎生殖细胞培养的影响[J]. , 2013, (7): 137-142.

DONG Xiao, FENG Shu-tang, WANG Hong-jun. The Effect on the Culturing of Porcine Embryonic Germ Cells by Genetic Background and Fetal Stage[J]. , 2013, (7): 137-142.

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