北京鸭抑制素α-亚基免疫原的制备及活性研究

›› 2012, Vol. 39 ›› Issue (7): 134-137.

北京鸭抑制素α-亚基免疫原的制备及活性研究

田锦

北京农业职业学院,北京 102442

收稿日期:2011-12-14 出版日期:2012-07-20 发布日期:2012-07-16
作者简介:田锦(1975－),女,内蒙古人,讲师,硕士,主要从事分子遗传学研究。
基金资助:
北京农业职业学院技术攻关与重点支持基金项目资助(yjxyf09-18)。

Study on Preparation and Immunocompetence of Inhibin Alpha-subunit in Beijing Duck

TIAN Jin

Beijing Vocational College of Agriculture,Beijing 102442,China

Received:2011-12-14 Online:2012-07-20 Published:2012-07-16

摘要/Abstract

摘要： 将重组有北京鸭抑制素α-亚基基因的T载体和pET-28a表达载体分别用NdeⅠ和XhoⅠ双酶切后连接并转化E. coli BL21(DE3),经PCR和双酶切鉴定,筛选得到阳性重组表达载体,在IPTG诱导下融合表达了北京鸭抑制素α-亚基蛋白。试验结果表明,抑制素α-亚基基因在E. coli BL21中得到了有效表达,表达产物具有免疫活性,为后期探讨该免疫原提高北京鸭产蛋性能的研究奠定基础。

关键词: 北京鸭; 抑制素α-亚基基因; 免疫活性

Abstract: pMD18-T vector with the inhibin α-subunit gene from Beijing duck and pET-28a expression vector were cut by NdeⅠ and XhoⅠ. The products were connected and transformated into E. coli BL21(DE3). The positive recombinant expression vectors were identificated by PCR and double digestion experiment. The inhibin α-subunit protein was induced by the IPTG. Tests in mice showed that inhibin α-subunit expressed effectively in E.coli BL21 had immunological competence. The results are helpful for the later research about improving the laying performance of Beijing duck.

Key words: Beijing duck; inhibin α-subunit gene; immunocompetence

中图分类号:

Q579.1

田锦. 北京鸭抑制素α-亚基免疫原的制备及活性研究[J]. , 2012, 39(7): 134-137.

TIAN Jin. Study on Preparation and Immunocompetence of Inhibin Alpha-subunit in Beijing Duck[J]. China Animal Husbandry & Veterinary Medicine, 2012, 39(7): 134-137.

参考文献

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