北京鸭DHAV-3抗性与易感品系的脂质轮廓鉴定与分析

doi:10.16431/j.cnki.1671-7236.2024.06.001

摘要/Abstract

摘要： 【目的】试验旨在研究抗鸭甲肝病毒基因3型(Duck hepatitis A virus genotype 3,DHAV-3)的北京鸭专门化品系(即抗性品系Z7-R)与DHAV-3易感品系(Z7-S)的脂质代谢轮廓,并筛选品系间差异脂质标志物。【方法】选取2日龄Z7系DHAV-3抗性北京鸭和易感北京鸭各6只,采集血液与肝脏组织,进行血液生化指标测定与基于液相色谱-质谱联用技术(LC-MS)的非靶向肝脏脂质组检测。采用t检验、偏最小二乘分析(PLS-DA)和差异倍数(FC)综合筛选品系间显著差异脂质。【结果】Z7-R系北京鸭血浆总胆固醇、低密度脂蛋白和磷脂含量均显著高于Z7-S系(P＜0.05)。脂质组分析共鉴定到1 532个脂质代谢物,涵盖了脂肪酰(FA)、甘油酯(GL)、甘油磷脂(GP)、鞘脂(SP)、固醇脂(ST)5个大类。共筛选到84个显著差异脂质,其中甘油酯类主要为甘油三酯(TG),且Z7-R系含量均高于Z7-S系(P＜0.05);甘油磷脂类主要为溶血磷脂酰胆碱(LPC)、溶血磷脂酰乙醇胺(LPE)和游离脂肪酸(FFAs),且Z7-R系含量均低于Z7-S系(P＜0.05)。共筛选到10个显著差异的脂质显著富集到鞘脂代谢、甘油磷脂代谢、甘油酯代谢、亚油酸代谢和α-亚麻酸代谢通路中。【结论】脂质组学技术可用于区分北京鸭Z7-R系与Z7-S系,筛选到的10个显著差异脂质物质可作为潜在的DHAV-3抗性与易感性状相关生物标志物。

关键词: 北京鸭; 鸭甲肝病毒基因3型; 脂质组; 代谢

Abstract: 【Objective】 The purpose of this experiment was to study the lipid metabolism profiles of the resistant Pekin duck line (Z7-R) and susceptible line (Z7-S) of Pekin ducks to Duck hepatitis A virus genotype 3 (DHAV-3),and investigate lipid markers with significant differences between this two lines.【Method】 6 2-day-old Z7-R and 6 2-day-old Z7-S Pekin ducks were selected and their blood and liver tissues were collected for blood biochemical index determination and non-targeted liver lipidomics detection based on liquid chromatography-mass spectrometry (LC-MS).t-test,partial least squares-discriminant analysis (PLS-DA) and fold change (FC) were used to comprehensively screen the significantly different lipids between the two lines.【Result】 The contents of total cholesterol,low density lipoprotein and phospholipid in the plasma of Z7-R Pekin duck were significantly higher than those in Z7-S (P＜0.05).A total of 1 532 lipid metabolites were identified by lipidome analysis,covering five major categories:Fatty acyl (FA),glycerolipids (GL),glycerophospholipids (GP),sphingolipids (SP),and sterol lipids (ST).A total of 84 significantly different lipids were identified.Triglyceride (TG) was the main component in glycerolipids,and the content of TG in Z7-R Pekin duck was significantly higher than that in Z7-S (P＜0.05).Lysophosphatidylcholine (LPC),lysophosphatidylethanolamine (LPE) and free fatty acid (FFAs) were the significantly different glycerophospholipids,and the contents of these lipids in Z7-R Pekin duck were significantly lower than those in Z7-S (P＜0.05).A total of 10 significantly different lipids were screened and they were significantly enriched in sphingolipid metabolism,glycerophospholipid metabolism,glyceride metabolism,linoleic acid metabolism and α-linolenic acid metabolism.【Conclusion】 Lipidomics technology could be used to differentiate Z7-R lines from Z7-S lines of Beijing ducks.The 10 significantly different lipids could be used as potential biomarkers related to DHAV-3 resistant and susceptible traits.

Key words: Pekin duck; DHAV-3; lipidomics; metabolism

中图分类号:

S813.2

常卓, 梁素芸, 王帅钦, 杨宇泽, 薛振华, 赵春颖, 林逍然, 路永强, 侯水生. 北京鸭DHAV-3抗性与易感品系的脂质轮廓鉴定与分析[J]. 中国畜牧兽医, 2024, 51(6): 2253-2260.

CHANG Zhuo, LIANG Suyun, WANG Shuaiqin, YANG Yuze, XUE Zhenhua, ZHAO Chunying, LIN Xiaoran, LU Yongqiang, HOU Shuisheng. Identification and Analysis of Lipid Profile in DHAV-3 Resistant and Susceptible Lines of Pekin Duck[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(6): 2253-2260.

参考文献

[1] ZHOU Z,LI M,CHENG H,et al.An intercross population study reveals genes associated with body size and plumage color in ducks[J].Nature Communications,2018,9(1):2648.
[2] WANG X,ZHANG J,MENG R,et al.Host differences affecting resistance and susceptibility of the second generation of a Pekin duck flock to Duck hepatitis A virus genotype 3[J].Frontiers in Microbiology,2017,8:1128.
[3] LIANG S,HU X,GUO Z,et al.Comparative transcriptome reveals the effect of IFITM1 on differential resistance to Duck hepatitis A virus genotype 3 in Pekin ducks[J].Virus Research,2022,322:198930.
[4] LIANG S,WANG M S,ZHANG B,et al.NOD1 is associated with the susceptibility of Pekin duck flock to Duck hepatitis A virus genotype 3[J].Frontiers in Immunology,2021,12:766740.
[5] LIN S L,CONG R C,ZHANG R H,et al.Circulation and in vivo distribution of Duck hepatitis A virus types 1 and 3 in infected ducklings[J].Archives of Virology,2016,161(2):405-416.
[6] DOAN H T T,LE X T K,DO R T,et al.Molecular genotyping of Duck hepatitis A viruses (DHAV) in Vietnam[J].The Journal of Infection in Developing Countries,2016,10(9):988-995.
[7] SHEN Y,CHENG A,WANG M,et al.Development of an indirect ELISA method based on the VP3 protein of Duck hepatitis A virus type 1 (DHAV-1) for dual detection of DHAV-1 and DHAV-3 antibodies[J].Journal of Virological Methods,2015,225:30-34.
[8] FU Y,PAN M,WANG X,et al.Molecular detection and typing of Duck hepatitis A virus directly from clinical specimens[J].Veterinary Microbiology,2008,131(3):247-257.
[9] YUGO D M,HAUCK R,SHIVAPRASAD H L,et al.Hepatitis virus infections in poultry[J].Avian Diseases,2016,60(3):576-588.
[10] 张姣姣,江勇,王笑言,等.抗甲肝3型病毒北京鸭选育初探[J].中国畜牧杂志,2016,52(17):5-8. ZHANG J J,JIANG Y,WANG X Y,et al.The preliminary study of breeding for Peking ducks resistance to Duck hepatitis A virus type 3[J].Chinese Journal of Animal Science,2016,52(17):5-8.(in Chinese)
[11] LIANG S,TANG J,WANG X,et al.Effect of Duck hepatitis A virus genotype 3 infection on glucose metabolism of Pekin ducklings and underlying mechanism[J].Gene,2020,748:144710.
[12] CAO J,ZHANG Y,CHEN Y,et al.Dynamic transcriptome reveals the mechanism of liver injury caused by DHAV-3 infection in Pekin duck[J].Frontiers in Immunology,2020,11:568565.
[13] LIANG S,XIE M,TANG J,et al.Proteomics reveals the effect of type Ⅰ interferon on the pathogenicity of Duck hepatitis A virus genotype 3 in Pekin ducks[J].Veterinary Microbiology,2020,248:108813.
[14] WANG X,DING D,LIU Y,et al.Plasma lipidome reveals susceptibility and resistance of Pekin ducks to DHAV-3[J].International Journal of Biological Macromolecules,2023,253(Pt 5):127095.
[15] WISHART D S.Metabolomics for investigating physiological and pathophysiological processes[J].Physiological Reviews,2019,99(4):1819-1875.
[16] 中国农业科学院北京畜牧兽医研究所.一种鉴定北京鸭抗DHAV-3性状能力的方法:202210852265.9[P].2022-10-14. INSTITUTE OF ANIMAL SCIENCE,CHINESE ACADEMY OF AGRICULTURAL SCIENCES.A method for identifying the ability of Beijing ducks to resist DHAV-3 traits:202210852265.9[P].2022-10-14.(in Chinese)
[17] 张润,刘海,杨曼,等.北京黑猪肌内脂肪含量高、低组间脂质组差异分析[J].畜牧兽医学报,2022,53(9):3262-3271. ZHANG R,LIU H,YANG M,et al.Analysis of lipidome difference between high and low intramuscular fat content groups in Beijing Black pigs[J].Chinese Journal of Animal and Veterinary Sciences,2022,53(9):3262-3271.(in Chinese)
[18] VAN D S H M,DOROBANTU C M,ALBULESCU L,et al.Fat(al) attraction:Picornaviruses usurp lipid transfer at membrane contact sites to create replication organelles[J]. Trends in Microbiology,2016,24(7):535-546.
[19] LANGE P T,LAGUNOFF M,TARAKANOVA V L.Chewing the fat:The conserved ability of DNA viruses to hijack cellular lipid metabolism[J].Viruses.2019,11(2):119.
[20] HERTZOG P.Viruses 'chew the fat’!?[J].Immunology and Cell Biology,2014,92(2):100-102.
[21] CAPUTA G,MATSUSHITA M,SANIN D E,et al.Intracellular infection and immune system cues rewire adipocytes to acquire immune function[J].Cell Metabolism,2022,34(5):747-760.e6.
[22] LI F,XIANG B,JIN Y,et al.Hepatotoxic effects of inhalation exposure to polycyclic aromatic hydrocarbons on lipid metabolism of C57BL/6 mice[J].Environment International,2020,134:105000.
[23] GAO Y,LIU Y,MA F,et al.Lactobacillus plantarum Y44 alleviates oxidative stress by regulating gut microbiota and colonic barrier function in BALB/c mice with subcutaneous D-galactose injection[J].Food & Function,2021,12(1):373-386.
[24] QIAN M,HU H,YAO Y,et al.Coordinated changes of gut microbiome and lipidome differentiates nonalcoholic steatohepatitis (NASH) from isolated steatosis[J].Liver International,2020,40(3):622-637.
[25] QIAO J T,CUI C,QING L,et al.Activation of the STING-IRF3 pathway promotes hepatocyte inflammation,apoptosis and induces metabolic disorders in nonalcoholic fatty liver disease[J].Metabolism:Clinical and Experimental,2018,81:13-24.
[26] YU Z,CHENG M,LUO S,et al.Comparative lipidomics and metabolomics reveal the underlying mechanisms of taurine in the alleviation of nonalcoholic fatty liver disease using the aged laying hen model[J].Molecular Nutrition & Food Research,2023,67(24):e2200525.
[27] CAI X,BAO L,WANG N,et al.Dietary nucleotides supplementation and liver injury in alcohol-treated rats:A metabolomics investigation[J].Molecules,2016,21(4):435.
[28] ZHU J Y,CHEN M,MU W J,et al.Higd1a facilitates exercise-mediated alleviation of fatty liver in diet-induced obese mice[J].Metabolism: Clinical and Experimental,2022,134:155241.
[29] LIU J X,LIU M,YU G Z,et al.Clonorchis sinensis infection induces hepatobiliary injury via disturbing sphingolipid metabolism and activating sphingosine 1-phosphate receptor 2[J].Frontiers in Cellular and Infection Microbiology,2022,12:1011378.
[30] SEHRAWAT T S,ARAB J P,LIU M,et al.Circulating extracellular vesicles carrying sphingolipid cargo for the diagnosis and dynamic risk profiling of alcoholic hepatitis[J].Hepatology,2021,73(2):571-585.
[31] YANG Y,LI F,WEI S,et al.Metabolomics profiling in a mouse model reveals protective effect of Sancao granule on Con A-induced liver injury[J].Journal of Ethnopharmacology,2019,238:111838.
[32] CASTRO-GÓMEZ P,GARCIA-SERRANO A,VISIOLI F,et al.Relevance of dietary glycerophospholipids and sphingolipids to human health[J].Prostaglandins,Leukotrienes and Essential Fatty Acids,2015,101:41-51.
[33] LU J,CHEN B,CHEN T,et al.Comprehensive metabolomics identified lipid peroxidation as a prominent feature in human plasma of patients with coronary heart diseases[J].Redox Biology,2017,12:899-907.